Effects of Salinity Fluctuation Frequency on the Growth, Molting Rate and Hemolymph 20-Hydroxyecdysone Concentration in Juvenile Chinese shrimp, Fenneropenaeus chinensis

This experiment was conducted to examine the effects of salinity fluctuation frequency on the growth, molting rate and hemolymph 20-hydroxyecdysone (20-HE) concentration for juvenile Fenneropenaeus chinensis with an initial wet body weight of 1.115 g±0.012 g. The salinity of the control treatment (represented by D0) was 28 throughout the experiment, whereas treatments D2,D4, D6 and D8 were subjected to different salinity fluctuation frequencies of 2, 4, 6 and 8d, respectively. The range of salinity fluctuation was 4. The salinity in treatments D2, D4, D6 and D8 was kept at 28 for 2, 4, 6 and 8d, respectively; each original salinity was decreased abruptly to salinity 24, which first lasted for another 2d and was then raised to its initial value 28. This constituted a salinity fluctuation cycle and afterwards the cycle repeated. It was found that: 1) The weight gain of shrimp in treatment D4 was 202.9% and significantly higher than that in treatment D0 (P<0.05). The molting rate in treatment D2 was the lowest, while that in treatment D6 was the highest and significantly higher than those in treatments D0 and D2 (P<0.05). 2) The hemolymph 20-HE concentration of shrimps in all treatments was at low levels and increased first gradually during the inter-molt period and then increased sharply. It reached a peak value during the pre-molt stage, and then decreased abruptly during the post-molt stage when the lowest level occurred. 3) During the post-molt stage, the hemolymph 20-HE concentration in treatment D2 was 7.47pgμL-1 and significantly higher than that in treatment D0(P<0.05). There were, however, no significant differences in hemolymph 20-HE concentration among all five treatments during the inter-molt stage (P>0.05). During the pre-molt stage, the hemolymph 20-HE concentration in treatment D6 was significantly higher than that in treatment D0(P<0.05), whereas the hemolymph 20-HE concentration in treatment D2 was significantly lower than that in other treatments (P<0.05). Adequate salinity fluctuation promoted the molting rate through increasing hemolymph 20-HE concentration at the pre-molt stage.     

临沂市加强土地开发整理推动新农村建设

土地开发整理与新农村建设相结合,是当前社会关注的热点之一。临沂市高度重视通过土地整理促进新农村建设,自2003年以来,共实施国家和省、市、县四级土地开发整理项目1335个,累计投入11．12亿元,项目总规模14．4万hm^,新增耕地2．2万hm^2。通过实施土地开发整理,新建灌溉渠系1930km,排水沟2315km,新上泵站313个,农用井661眼,新上输变电线路287km,     

Discrete time-delay control of an autonomous underwater vehicle: Theory and experimental results

A discrete time-delay control (DTDC) law for a general six degrees of freedom unsymmetric autonomous underwater vehicle (AUV) is presented. Hydrodynamic parameters like added mass coefficients and drag coefficients, which are generally uncertain, are not required by the controller. This control law cancels the uncertainties in the AUV dynamics by direct estimation of the uncertainties using time-delay estimation technique. The discrete-time version of the time-delay control does not require the derivative of the system state to be measured or estimated, which is required by the continuous-time version of the controller. This particularly provides an advantage over continuous-time controller in terms of computational effort or availability of sensors for measuring state derivatives, i.e., linear and angular accelerations. Implementation issues for practical realization of the controller are discussed. Experiments on a test-bed AUV were conducted in depth, pitch, and yaw degrees of freedom. Results show that the proposed control law performs well in the presence of uncertainties.     

Numerical study of baroclinic tides in Luzon Strait

The spatial and temporal variations of baroclinic tides in the Luzon Strait (LS) are investigated using a three-dimensional tide model driven by four principal constituents, O₁, K₁, M₂ and S₂, individually or together with seasonal mean summer or winter stratifications as the initial field. Barotropic tides propagate predominantly westward from the Pacific Ocean, impinge on two prominent north-south running submarine ridges in LS, and generate strong baroclinic tides propagating into both the South China Sea (SCS) and the Pacific Ocean. Strong baroclinic tides, ∼19 GW for diurnal tides and ∼11 GW for semidiurnal tides, are excited on both the east ridge (70%) and the west ridge (30%). The barotropic to baroclinic energy conversion rate reaches 30% for diurnal tides and ∼20% for semidiurnal tides. Diurnal (O₁ and K₁) and semidiurnal (M₂) baroclinic tides have a comparable depth-integrated energy flux 10–20 kW m⁻¹ emanating from the LS into the SCS and the Pacific basin. The spring-neap averaged, meridionally integrated baroclinic tidal energy flux is ∼7 GW into the SCS and ∼6 GW into the Pacific Ocean, representing one of the strongest baroclinic tidal energy flux regimes in the World Ocean. About 18 GW of baroclinic tidal energy, ∼50% of that generated in the LS, is lost locally, which is more than five times that estimated in the vicinity of the Hawaiian ridge. The strong westward-propagating semidiurnal baroclinic tidal energy flux is likely the energy source for the large-amplitude nonlinear internal waves found in the SCS. The baroclinic tidal energy generation, energy fluxes, and energy dissipation rates in the spring tide are about five times those in the neap tide; while there is no significant seasonal variation of energetics, but the propagation speed of baroclinic tide is about 10% faster in summer than in winter. Within the LS, the average turbulence kinetic energy dissipation rate is O(10⁻⁷) W kg^{− 1} and the turbulence diffusivity is O(10⁻³) m²s⁻¹, a factor of 100 greater than those in the typical open ocean. This strong turbulence mixing induced by the baroclinic tidal energy dissipation exists in the main path of the Kuroshio and is important in mixing the Pacific Ocean, Kuroshio, and the SCS waters.     

一种水不溶灰树花菌丝体多糖(GF4A)硫酸酯化研究

以灰树花发酵菌丝体水不溶性多糖GF4A为原料,以三氧化硫三甲胺盐(SO3·Me3N)为酯化试剂,探讨了GF4A的硫酸酯化工艺.实验结果表明,当三氧化硫与GF4A中单糖摩尔比为5.8∶1、反应温度为80 ℃、反应时间6 h时,所得酯化产物(SGF4A)水溶性好、产率高.该产物硫酸酯取代度为1.6, 相对分子质量为88 kD.通过红外光谱及核磁共振碳谱分析表明,糖残基中所有C-6羟基以及部分C-4和C-2位羟基被酯化.体外实验表明,SGF4A具有较好的抗凝活性.     

Optimum temperature for the growth performance of juvenile orange-spotted grouper （Epinephelus coioides H.）

Effects of water temperature （17, 21, 25, 30 and 35℃） and body size （14.75-281.41 g initial body weight） on food consumption, growth, feed conversion, and dry matter content in orange-spotted grouper fed to satiation were investigated. The combined effect of temperature （T, ℃） and body weight （W, g） on maximum food consumption （Cmax, g/day） was described as： InCmax=-7.411＋0.828 InW＋0.317T4）.004 7T2, and the optimum feeding temperature was 33.9℃. The combined effect of temperature and body weight on growth （G） was described as： lnG=-4.461-0.2081nW＋0.394T-0.006 3T^2. The optimum growth temperature was 31.4℃, whereas overall growth rates were high at 25, 30 and 35 ℃. Feed conversion efficiencies （FCE, %）, increasing first and then decreasing with increasing temperature, averaged from 1.8 to 2.1 in terms of dry weight of food fish. The optimum temperature for FCE tended to be lower than that for growth or feeding. Dry matter content increased with both increasing water temperature （17, 25, 30 and 35℃） and body weight, and the combined effect of temperature and body weight on dry matter content （DM, %） was described as： lnDM =3.232＋0.01 4 lnW-0.004 4T＋0.001 2TInW.     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

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Field detection of chlorophyll-a concentration in the sea surface layer by an airborne oceanographic lidar

An airborne oceanographic lidar, with a frequency-tripled Q-switched Nd： YAG laser of 355 nm, has been designed to measure chlorophyll-a （Chl-a） concentration in the sea surface layer by the Ocean Remote Sensing Institute, OUC. The field experiment was carried out in the bay which is located south of the Liaodong Peninsula on the 10th of September 2005. After the flight, the raw data were processed and analyzed by the fluorescence-to-Raman ratio method with seawater attenuation coefficients calculated from signal profiles. The results of Chl-a concentration sea water were also compared with those of Chl-a concentration by measurements by lidar are shown. The measurements in clear a Moderate Resolution Imaging Spectroradiometer （MODIS）.     

Field Detection of Chlorophyll-a Concentration in the Sea Surface Layer by an Airborne Oceanographic Lidar

An airborne oceanographic lidar, with a frequency-tripled Q-switched Nd: YAG laser of 355 nm, has been designed to measure chlorophyll-a (Chl-a) concentration in the sea surface layer by the Ocean Remote Sensing Institute, OUC. The field experiment was carried out in the bay which is located south of the Liaodong Peninsula on the 10th of September 2005. After the flight, the raw data were processed and analyzed by the fluorescence-to-Raman ratio method with seawater attenuation coefficients calculated from signal profiles. The results of Chl-a concentration measurements by lidar are shown. The measurements in clear sea water were also compared with those of Chl-a concentration by a Moderate Resolution Imaging Spectroradiometer (MODIS).