一种水不溶灰树花菌丝体多糖(GF4A)硫酸酯化研究

以灰树花发酵菌丝体水不溶性多糖GF4A为原料,以三氧化硫三甲胺盐(SO3·Me3N)为酯化试剂,探讨了GF4A的硫酸酯化工艺.实验结果表明,当三氧化硫与GF4A中单糖摩尔比为5.8∶1、反应温度为80 ℃、反应时间6 h时,所得酯化产物(SGF4A)水溶性好、产率高.该产物硫酸酯取代度为1.6, 相对分子质量为88 kD.通过红外光谱及核磁共振碳谱分析表明,糖残基中所有C-6羟基以及部分C-4和C-2位羟基被酯化.体外实验表明,SGF4A具有较好的抗凝活性.     

Optimum temperature for the growth performance of juvenile orange-spotted grouper （Epinephelus coioides H.）

Effects of water temperature （17, 21, 25, 30 and 35℃） and body size （14.75-281.41 g initial body weight） on food consumption, growth, feed conversion, and dry matter content in orange-spotted grouper fed to satiation were investigated. The combined effect of temperature （T, ℃） and body weight （W, g） on maximum food consumption （Cmax, g/day） was described as： InCmax=-7.411＋0.828 InW＋0.317T4）.004 7T2, and the optimum feeding temperature was 33.9℃. The combined effect of temperature and body weight on growth （G） was described as： lnG=-4.461-0.2081nW＋0.394T-0.006 3T^2. The optimum growth temperature was 31.4℃, whereas overall growth rates were high at 25, 30 and 35 ℃. Feed conversion efficiencies （FCE, %）, increasing first and then decreasing with increasing temperature, averaged from 1.8 to 2.1 in terms of dry weight of food fish. The optimum temperature for FCE tended to be lower than that for growth or feeding. Dry matter content increased with both increasing water temperature （17, 25, 30 and 35℃） and body weight, and the combined effect of temperature and body weight on dry matter content （DM, %） was described as： lnDM =3.232＋0.01 4 lnW-0.004 4T＋0.001 2TInW.     

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)     

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Evaluation of daily precipitation in China from ECMWF and NCEP reanalyses

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Exercise in downscaling on sea surface temperature along Chinese coast

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Trends and risk evolution of drought disasters in Tibet Region,China

Journal of Geographical Sciences - The risk posed by natural disasters can be largely reflected by hazard and vulnerability. The analysis of long-term hazard series can reveal the mechanisms by...     

Benthic foraminiferal fauna and sediment provenance of Eocene syn-rift sequences in Taiwan: implication for onset of Asian epi-continental marginal seas off China coast

Marine Geophysical Research - Early Cenozoic rift basins developed commonly on the Mesozoic basement along the SE Asia Continent. However, Eocene–Oligocene sequences were only exposed widely...     

胶州湾沉积物中石油降解菌的降解能力及多样性研究

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。     

球面距离计算方法及精度比较

球面距离(角间距)计算是天文或地理学中极常用的计算之一,也是目标查找、锥形检索、交叉证认等方法的基础。数学上,通过球面几何可以直接计算出两点的距离,前人已经推导出了多个复杂程度不一的计算方法。但是由于计算机的精度有限,在进行数值计算时有舍入误差,导致公式计算结果出现偏差。对几个常用的球面距离计算公式进行了考察,测试并对比它们在不同计算环境下的精度与优缺点。此外还展示并比较了几种常用天文软件包、数据库的球面距离计算方法,以期有助于天文工作者选择适合自己当前需要的计算方法。