Biomarkers of Exposure and Effects for Assessing Toxicological Risk of Endocrine Disrupters in Top Predators of the Mediterranean Sea

Abstract. Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fisheries. In this research the unexplored hypothesis that Mediterranean top predator species (such as large pelagic fish and marine mammals) are potentially at risk due to EDCs is investigated. In the Mediterranean environment, top predators accumulate high concentrations of polyhalogenated aromatic hydrocarbons (PHAHs) and toxic metals, incurring high toxicological risk. Here we illustrate the need to develop sensitive biomarkers for evaluation of toxicological risk in top marine predators (Xiphias gladius, Thunnus thynnus thynnus) and non-lethal techniques, such as non-destructive biomarkers, for the hazard assessment of threatened species exposed to EDCs, such as marine mammals ( Stenella coeruleoalba, Tursiops truncatus, Delphinus delphis and Balaenoptera physalus ).     

Stratigraphic framework of gas accumulations in the Ría de Pontevedra (NW Spain)

Knowledge of marine geological environments in which shallow gas is accumulating is becoming increasingly important in global studies of climate change because a measurable proportion of the total methane source comes from continental margins. Previous studies have revealed that coastal environments represent important geological environments where microbial methane is being generated, is accumulating, and is being released. In the Ría de Pontevedra, at least 4.5 km² of seafloor in the innermost part of the ría is underlain by sediments containing natural gas. Seismic interpretation contributes new findings for the definition of periods and geological environments in which the gas could have been generated, and is accumulating and released in the Ría de Pontevedra. Groundtruthing the seismic data (facies, environments) makes it possible to identify favourable geological environments for gas generation in the sedimentary infill of the Ría de Pontevedra. Sequence stratigraphy based on high-resolution seismic profiles and post-Last Glacial Maximum sea-level records makes it possible to establish the stratigraphic architecture of the ría and to define the periods in which gas could have been generated. The results of this study show that the sedimentary infill is composed of a fifth-order sequence developed since the Last Glacial Maximum. Within this sequence, gas appears to have accumulated in the Holocene deposits associated with the latest transgressive and highstand system tracts. Seismic analysis shows that gas could have been generated in different geological environments in the Ría de Pontevedra. If coastal environments at times of lower sea level were similar to those of the present, organic-rich mud deposits (deposited mainly in lakes, estuaries and floodplains) could have survived transgression and remained buried as potential gas sources in the inner part of the ría.     

Assessment of dissolved nutrients dispersal derived from offshore fish-farm using nitrogen stable isotope ratios (δ15N) in macroalgal bioassays

In this study, the dispersal of wastes from offshore fish farms was evaluated by analyzing nitrogen stable isotope ratios (δ¹⁵N) in macroalgae incubated in the water column at sites located at an increasing distance from the fish cages. Bioassays were performed at three fish farms situated in separate localities with different nutritional conditions (Canary Islands, Murcia and Catalonia) and varying in size, species of fish reared and annual production. Macroalgal bioassays were carried out in two different directions (DI and DII) and they were replicated at each distance in order to evaluate the effect of small-scale variability on the spatial extent of fish farm wastes. The results obtained with δ¹⁵N contribute to a better understanding of the application of nitrogen stable isotopes ratios in macroalgae as an effective bioindicator for tracing the dispersion of offshore fish farm wastes, and demonstrate that fish farm wastes can be traced even over distances of some km from the pollution source. In the Canary Islands, the maximum distance obtained for detection of fish farm wastes was between 450 and 700 m. Of the three installations studied, Murcia presented the greatest distance for detection of fish farm waste influence, ranging from between 1550 and 2450 m, whilst in Catalonia this distance was less than 120 m. In Catalonia, the results were masked by the influence of other sources of nitrogen, and thus fish farm wastes were detected at more reduced distances than expected. These results confirm that fish farm wastes can be traced using the nitrogen stable isotope ratios of macroalgae and that this method can also be useful for identifying areas of potential risk to some sensitive ecosystems, and as an early signal that changes in the community structure might occur.     

Microdistribution of macroinvertebrates in a temporary pond of Central Italy: Taxonomic and functional analyses

Spatial distribution of physical and chemical variables and macroinvertebrate composition, structure and functional aspects were investigated in five microhabitats available (Ranunculus acquatilis+Ranunculus sardous, Spirogyra sp., Juncus effusus, and unvegetated littoral sediments and central sediments) in a temporary pond near Rome during spring 2004. The central sediments were found to differ greatly from the other substrates. They were characterized by higher nutrient contents (total P, total N), organic matter and organic C, and silt and clay in the sediments, and lower dissolved oxygen content and lower pH in the water. Species richness and densities of total macrofauna showed the lowest values in central sediments and the highest ones in submerged macrophytes (Ranunculus spp.) and emergent vegetation (Juncus effusus). Oligochaeta Tubificidae, some Nematoda (Dorylaimus spp.), and Chironomidae Tanypodinae (Procladius sp. and Psectrotanypus varius) and Chironominae (Chironomus plumosus group) characterized the central sediments, whereas Ephemeroptera and most of the Odonata and Coleoptera species were commonly found in submerged macrophyte beds. Some species of Coleoptera and Hemiptera (Hygrobia hermanni, Helochares lividus, Berosus signaticollis and Gerris maculatus) were mainly found in the algal substratum, and some Nematoda species (Tobrilus spp. and Aporcelaimellus obtusicaudatus), Oligochaeta Enchytraeidae, young larvae of Sympetrum and Diptera Ceratopogonidae in littoral sediments. Juncus effusus appeared to be mainly colonized by Chironomidae Orthocladiinae (Psectrocladius sordidellus group and Corynoneura scutellata) and Tanytarsini (Paratanytarsus sp.). Central sediments also favoured high abundances of collector-gatherers, burrowers and drought resistant forms with passive dispersal, whereas Ranunculus spp. hosted mainly scrapers, shredders, swimmers+divers and active dispersal forms without any resistant stages to desiccation. Juncus plants were mostly colonized by collector-filterers and by organisms capable of both active dispersal and surviving desiccation. Littoral sediments and algae showed similar functional organization and intermediate features between central sediments and submerged macrophyte beds. All these results demonstrate that microhabitat characteristics play a crucial role in selecting macroinvertebrate taxa according to their environmental requirement, feeding mechanism, movement and resistance to drought. Moreover, our study confirms the role of submerged and emergent vegetation in maintaining high biodiversity and suggests that all microhabitats should be considered to provide both an exhaustive collection of species for pond management and conservation and basic insights into the functioning of pond communities.     

Location,location, location: changes in the diversity of animal resources exploited by Tardiglacial humans in northern Spain

Prey diversity of Homo sapiens in north Iberia exhibited shifts associated with climate warming after the Last Glacial Maximum, as deduced from animal remains obtained from Upper Palaeolithic caves in Asturias (northern Spain). Significant association of prey diversity and river proximity was found during adverse climate conditions, indicating opportunistic predation. A high increase of prey diversity was found when small animals (including fish) were taken into account in archaeological records, highlighting the importance of this type of prey in Palaeolithic diets. Copyright © 2009 John Wiley & Sons, Ltd.     

Landform instability and land-use dynamics in tropical high mountains, Central Mexico

This investigation is an analysis of the influence of landform instability on the distribution of land-use dynamics in a hydrographical basin, located in the Mexican Volcanic Belt mountain range (central Mexico), currently affected by substantial changes in land use and deforestation. A landform map was produced, in addition to seven attribute maps - altimetry, drainage density, slope, relief energy, potential erosion, geology and tectonics - which were considered as factors for determining landform instability through Multi-criteria Evaluation Analysis. Likewise, the direction and rhythm of land-use dynamics were analyzed in four dates - between 1976 and 2000 - and cross tabulations were made between them, in order to analyze the trends and processes of land-use dynamics. Afterwards, the databases obtained were cross tabulated with the landform variables to derive areas, percentages and correlation indices. In the study area, high-instability landforms are associated with most ancient volcanic and sedimentary landforms, where high altitude, drainage density, slope and potential to develop gravitational and fluvial processes are the major factors favouring a land-use pattern, dominated by the conservation of extensive forest land, abandonment of human land use and regeneration of disturbed areas. In contrast, low-instability landforms correspond to alluvial plains and lava hills covered by pyroclasts, where low potential erosion to develop fluvial processes, added to water and soil availability and accessibility, have favoured a land-use pattern dominated by the expansion of agroforestry plantations and human settlements, showing a marked trend towards either intensification or permanence of the current land use and with little abandonment and regeneration.     

A Relationship Between Transition Region Brightenings,Abundances, and Magnetic Topology

We present multi-instrument observations of active region (AR) 8048, made between 3 June and 5 June 1997, as part of the SOHO Joint Observing Program 33. This AR has a sigmoid-like global shape and undergoes transient brightenings in both soft X-rays and transition region (TR) lines. We compute a magneto-hydrostatic model of the AR magnetic field, using as boundary condition the photospheric observations of SOHO/MDI. The computed large-scale magnetic field lines show that the large-scale sigmoid is formed by two sets of coronal loops. Shorter loops, associated with the core of the SXT emission, coincide with the loops observed in the hotter CDS lines. These loops reveal a gradient of temperature, from 2 MK at the top to 1 MK at the ends. The field lines most closely matching these hot loops extend along the quasi-separatrix layers (QSLs) of the computed coronal field. The TR brightenings observed with SOHO/CDS can also be associated with the magnetic field topology, both QSL intersections with the photosphere, and places where separatrices issuing from bald patches (sites where field lines coming from the corona are tangent to the photosphere) intersect the photosphere. There are, furthermore, suggestions that the element abundances measured in the TR may depend on the type of topological structure present. Typically, the TR brightenings associated with QSLs have coronal abundances, while those associated with BP separatrices have abundances closer to photospheric values. We suggest that this difference is due to the location and manner in which magnetic reconnection occurs in two different topological structures. Supplementary material to this paper is available in electronic form at http://dx.doi.org/10.1023/A:1013302317042     

Testing the consistency of the Starburst scenario for active galactic nuclei

In the frame of the Starburst Model, we show that the evolution of a massive stellar cluster in a high metallicity environment can reproduce the observed emission-line spectrum and the UV-optical Spectral Energy Distribution of the Seyfert 2 galaxies and LINERs. We apply the results of our models to three objects: NGC 5506, NGC 5643 and Mk 348.