Contamination of organotin compounds and imposex in molluscs from Vancouver,Canada

Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study.     

Effects of prolonged exposure to 4-tert-octylphenol on toxicity and indices of oestrogenic exposure in the sand goby (Pomatoschistus minutus, Pallas)

Alkylphenolic compounds are present in estuarine and marine environments. They are moderately bioaccumulative, and oestrogenic to fish following three week, in vivo, exposures. The effects of prolonged exposure are, as yet, unclear. Sand goby (Pomatoschistus minutus) were exposed to measured concentrations of 4-tert-octylphenol (OP) in a 28-day, dose-ranging study, and a six month temporal response study. Following 28-days exposure to measured 31+/-6 or 101+/-47 microg l(-1) OP (mean+/-SD), immature male sand goby showed elevated vitellogenin (VTG) mRNA expression. Plasma alkali-labile phosphate concentrations were elevated in both sexes and were higher in females than males. Although measured OP concentrations were variable, they were significantly different for each treatment and a range of concentration-dependent effects were observed. The LC(50) for chronic OP exposure (8 weeks) was 29+/-6 microg l(-1), and both sexes demonstrated concentration and duration dependent increases in VTG mRNA expression. Exposure to 28+/-20 microg l(-1) OP (median+/-95% CI) for 6 months inhibited development of male nuptial colouration and sperm duct glands. These findings are discussed in relation to environmental concentrations of alkylphenols and ecological concerns.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

Estimation of Sea Ice Thickness from SARAL/AltiKa in Drifting Orbit Phase

Abstract

Intra and inter-annual variations in the sea ice thickness are highly sensitive indicators of climatic variations undergoing in the earth’s atmosphere and oceans. This paper describes the method of estimating sea ice thickness using radar waveforms data acquired by SARAL/Altika mission during its drifting orbit phase from July 2016 onwards yielding spatially dense data coverage. Based on statistical analysis of return echoes, classification of the surface has been carried out in three different types, viz. floe, lead and mixed. Time delay correction methods were suitably selected and implemented to make corrections in altimetric range measurements and thereby freeboard. By assuming hydrostatic equilibrium, freeboard data were converted into sea ice thickness. Results show that sea ice thickness varies from 4 to 5?m near ice shelves and 1 to 2.5?m in the marginal sea ice regions. Freeboard and sea ice thickness estimates were also validated using NASA’s Operation Ice Bridge (OIB) datasets. Freeboard measurements show very high correlation (0.97) having RMSE of 0.13. Overestimation of approximately 1–2?m observed in the sea ice thickness, which could be attributed to distance between AltiKa footprint and OIB locations. Moreover, sensitivity analysis shows that snow depth and snow density over sea ice play crucial role in the estimation of sea ice thickness.     

Occurrence of Nummulitic coralline limestone from offshore Palar Basin,Bay of Bengal,India

Abstract

Palar basin is located between Pennar and Cauvery sedimentary basins of East coast of India in Bay of Bengal, northeast Indian Ocean. Sea floor drill (Wire-line Autonomous Coring System – WACS) with operational capability of up to 3000?m water depth was developed to collect long cores from deep sea floor for geotechnical and ocean resource assessment studies. During the drilling operation it encountered Nummulitic coralline limestone of Lower Eocene age at 18 meters below the seafloor (mbsf) at 850?m water depth indicating carbonated platform presence for the first time at the study region. Bathymetry contour from Naval Hydrography Chart and General Bathymetric Chart of the Oceans (GEBCO) has revealed the presence of shallow mounds from 50 to 200?m depth closure contour near the sampling site at 850?m water depth which might be a submerged carbonated structure. Since, Nummulites are shallow water dwelling fauna (<20?m depth) but its occurrence at 18 mbsf in 850?m water depth is recorded because of the advancement in technology tool for long core sampling by means of sea floor drill.     

Distribution and transport of organic matter along a filament-eddy system in the Canaries – NW Africa coastal transition zone region

The distribution and transport of chlorophyll a (Chla), particulate (POC) and dissolved (DOC) organic carbon, and the respiratory ETS activity of the microplankton community were studied along a filament-eddy system located in the transition zone between the NW Africa upwelling and Canary Islands waters. Two independent filaments (F1 and F2) stemming from the coastal jet, between Cape Juby and Cape Bojador, merged about 100 km offshore, turning southward and onshore forced by the circulation of a recurrent oceanic cyclonic eddy. In general, the coastal upwelling waters presented higher Chla, but lower POC, DOC and ETS activity than filament waters. However, differences in organic carbon distribution and respiratory activity were observed among stations from the two filaments. The bio-chemical fields were strongly influenced by a complex sub-mesoscale hydrography resulting from the interaction of cyclonic and anticyclonic island eddies with the filaments. The combined F1 + F2 filament system transported 97.1 kg s⁻¹ of excess (non-refractory) total organic carbon (e-TOC), a value comparable to other published estimates from upwelling filaments in the NE Atlantic. About 90% of e-TOC was exported as DOC, since eddy re-circulation precluded the offshore transport of POC. Assuming that the calculated transport of e-TOC is representative of the annual average, the yearly offshore transport (3.1 x 10⁹ kg C) would represent about 25% of the upwelling primary production of the region of study.     

Artificial upwelling induced by ocean currents —Theory and experiment

A submerged apparatus, which consists of a buoy, several horizontal contraction and expansion tubes (Venturi-type tubes) and a long pipe, is expected to be used to pump the subsurface sea-water (200–300 meter depth) containing abundant nutrients to surface layer (50–100 m) by the dynamic of ocean currents. i.e. an artificial upwelling without energy cost. A preliminary experiment and analysis are undertaken and shows that the capacity of pumping the nutrient-rich sea-water is worth to build a pilot prototype model.     

Ridge development as revealed by sub-bottom profiles on the central New Jersey shelf

Closely-spaced 3.5 kHz seismic profiles were collected over the north-easterly trending ridge and swale system 50 km east-southeast of Atlantic City, New Jersey. They yield information on the Late Quaternary depositional history of the area, and on the origin of the ridge system. Four of the sub-bottom reflectors identified were sufficiently persistent to warrant investigation and interpretation. These reflectors, which have been cored, lithologically identified, and radiocarbon dated, are stratigraphically higher than the reflectors dealt with by the majority of previous studies. The upper three reflectors are definitely mid- and post-Wisconsin in age and present a record of the most recent glacial cycle. The upper three units associated with the observed reflectors appear to exert a pronounced influence on the bathymetry. The gently corrugated ridge system of Holocene sand is formed over the regionally flat-lying upper unit, an Early Holocene lagoonal silty clay. The characteristically flat, broad depressions of the area are floored by this lagoonal material. Locally, however, marine scour has cut through the silty clay into an underlying unit of unconsolidated fine Pleistocene sand. Several stages of trough development appear to be represented. After penetrating the lagoonal clay, troughs are initially narrow, but when incised through the sand into a lower, Pleistocene, silty-clay unit, the troughs become notably wider. As downcutting is inhibited by the lower clay, the upper clay is undercut as the trough widens in a fashion similar to a desert blowout.

The sub-bottom reflectors indicate that ridge development on the central shelf has involved aggradation as well as erosion. Some ridges seem to have grown by vertical and lateral accretion from small cores. The internal structure of other ridges suggests that they formed by the coalescence of several small ridges. Others appear to have undergone appreciable lateral migration.

The ridges appear to be in a state of continuing adjustment to the hydraulic regime of the deepening post-Pleistocene water column.     

Geopotential topography of deep levels in the Pacific Ocean

After examination of the baroclinic structure below 3000 db, recent data were used to map the geopotential topography at 1000, 1500, and 2000 db (referred to 3000 db) in the Pacific Ocean. In the high-latitude regions and in the western boundary currents, considerable relief is present at 1000 and 1500 db, and the Circumpolar Current is still well-developed at 2000 db. The geostrophic circulation inferred is very similar to the surface circulation in these areas. Neglect of the structure below 1000 db results in appreciable errors in estimates of the transport of these currents; direction of near-surface flow is properly shown, however, and computed speeds are not greatly in error. In the equatorial Pacific and central North Pacific, little relief is present, and the trends are generally unlike the surface flow.     

Estimation of winds over the sea from land-based measurements

Simultaneous measurements of wind velocities at two different sites, one over the sea and the other over land, can differ substantially and therefore cannot be interchanged. In situations where the wind data at an offshore site are missing while simultaneous measurements from a land-based station exist, a linear mean-square estimation (LMSE) technique can be used to estimate the missing data. This technique relies on past wind data gathered simultaneously at the two locations, and it generates from the associated correlation a set of four transfer functions capable of predicting one data set from the other. In the present case, the LMSE technique is outlined briefly, and is then applied to construct seasonal transfer functions between a land-based station and two coastal/offshore sites in Kuwait. Comparisons between the actually observed wind characteristics and those predicted by the LMSE technique are favorable, and thus tend to confirm the applicability of the technique under appropriate conditions.