海带雌性原生质体的分离与培养

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。     

Distribution of As, Cr and V species in the Po-Adriatic mixing area, (Italy)

The distribution of dissolved and participate concentrations of As, Cr and V was investigated in the mixing area of the Po river in five surveys over the period March 1992-March 1995. The data obtained indicate noticeable contamination of coastal waters of the northern Adriatic with Cr but not As or V. Total dissolved concentrations for As and V were comparable with oceanic levels but were higher by a factor of 4 for Cr. These results suggest either low anthropogenic inputs of As and V, from terrigenous and atmospheric sources, or efficient removal mechanisms. The investigated metals had low reactivity during freshwater-seawater mixing and the distribution of concentrations in the dissolved and solid phases suggests that the dilution process is the main factor controlling the transport of these metals to the sea in low-to-medium solid transport conditions. Further investigations are required to elucidate the behavior of these metals during high solid transport conditions. Significant changes in the speciation of As occurred during the transition between the two end-member matrices. In the coastal waters of the northern Adriatic, the arsenite fraction in winter, and the arsenite and dimethylarsinate fractions in summer increased with respect to riverine waters. Changes in the speciation of chromium were minor, but also showed a small increase in reduced species in marine waters.     

Environmental overview of Eckernförde Bay,northern Germany

This environmental overview of Eckernförde Bay (northern Germany) summarizes the results of previous studies relevant to the Office of Naval Research's Coastal Benthic Boundary Layer (CBBL) Baltic field exercise conducted during 1993–1994. Significant environmental characteristics include the following: (1) surface sediment distribution is related to water depth, dictated primarily by hydrodynamic reworking of older glacial deposits; (2) the origin and characteristics of small-scale sedimentary structures depend on storm-generated waves and currents; (3) the proximity of the sea surface and sediment —water interface results in a pelagic—benthic coupling that drives biogeochemical processes and produces organicrich, acoustically turbid sediments; and (4) the bay floor is complicated topographically by pockmarks and manmade sedimentary structures.     

Voracious planktonic hydroids: unexpected predatory impact on a coastal marine ecosystem

Hydroids are typically attached, benthic cnidarians that feed on a variety of small prey. During sampling on Georges Bank in spring 1994, we found huge numbers of hydroids suspended in the plankton. They fed on young stages of copepods that are an important prey for fish, as well as on young fish themselves. Two independent methods were used to estimate feeding rates of the hydroids; both indicate that the hydroids are capable of consuming from 50% to over 100% of the daily production of young copepods. These results suggest that hydroids can have a profound effect on the population dynamics of zooplankton and young fish on Georges Bank.     

Three-dimensional visualization of mission planning and control forthe NPS autonomous underwater vehicle

The Naval Postgraduate School (NPS) is constructing a small autonomous underwater vehicle (AUV) with an onboard mission control computer. The mission controller software for this vehicle is a knowledge-based artificial intelligence (AI) system requiring thorough analysis and testing before the AUV is operational. The manner in which rapid prototyping of this software has been demonstrated by developing a controller code on a LISP machine and using an Ethernet link with a graphics workstation to simulate the controller's environment is discussed. The development of a testing simulator using a knowledge engineering environment (KEE) expert system shell that examines AUV controller subsystems and vehicle models before integrating them with the full AUV for its test environment missions is discussed. This AUV simulator utilizes an interactive mission planning control console and is fully autonomous once initial parameters are selected     

Numerical simulation of unsteady free-surface motions using a boundary integral formulation

A numerical time simulation method is described to solve fluid flow problems including unsteady free surface motion. The method is based on potential flow theory. At every time step, the problem is solved using a boundary integral formulation of the fluid domain. The linearized free surface conditions are integrated in time and the solution is marched forward. Computational results simulating the free surface motion for the cases of a linear progressive wave, wave propagating into a region of calm water and the wave maker motion are presented. Comparison with theoretical results demonstrate the feasibility of the proposed simulation scheme.     

Bacterial sulphate reduction in sediments of a european salt marsh: Acid-volatile and tin-reducible products

The products of ³⁵S-sulphate reduction by sedimentary bacteria were measured at two sites in a salt marsh on the east coast of England. Non-acid-volatile products were measured, after acid-volatile sulphide was removed, by their reduction to sulphide by digestion with tin. The proportion of the sulphate reduced to tin-reducible products varied between 25% in a salt marsh pan and 61% in creek sediment, over a 0–25 cm depth profile. There were also variations with depth at each site in the proportions of sulphate reduced to tin-reducible products. Further examination revealed differences in the proportions of sulphate which were reduced to free sulphide, acid-volatile sulphide, sulphur or pyrite at the two sites. The data suggest that previous work which did not measure non-acid-volatile products underestimated sulphate reduction rates by three-fold in the creek site, but by only one third in the pan.     

The distributions of particulate atmospheric trace metals and mineral aerosols over the Indian Ocean

Data are presented for the concentrations of Al, Fe, Mn, Ni, Co, Cr, V, Cu, Zn, Pb and Cd in aerosols collected over two contrasting regions of the Indian Ocean. These are: (1) the northern Arabian Sea (AS), from which samples were collected in the northeast monsoon, during which the region receives an input of crustal material from the surrounding arid land masses; and (2) the Tropical Southern Indian Ocean (TSIO), a remote region from which samples were collected from air masses for which there were no large-scale up-wind continental aerosol sources. The TSIO samples can be divided into two populations: Population I aerosols, collected from air masses which have probably impinged on Madagascar, and Population II aerosols, which have been confined to open-ocean regions to the south of the area.The data indicate that there are strong latitudinal variations in the chemical signatures of aerosols over the Indian Ocean. The input of crustal material to the Arabian Sea gives rise to an average Al concentration of about 1000 ng m⁻³ of air in the northeast monsoon regime. As a result, the concentrations of all trace metals are relatively high, and the values of crustal enrichment factors are less than 10 for most metals, in the AS aerosols. In contrast, TSIO Population II ‘open-ocean southern air’ sampled during the southwest monsoon season, has an average Al concentration of only about 10 ng m⁻³ of air. Trace metal concentrations in the TSIO ‘open-ocean southern air’ during the southwest monsoon season are representative of ‘clean’ remote marine air and are generally similar to those reported over the central North Pacific.Mineral dust concentrations over the Indian Ocean decrease in a north to south direction, from about 15–20 μg m⁻³ of air in the extreme north to about 0.01–0.25 μg m⁻³ of air in the far south. The deposition of mineral dust over the northern Arabian Sea can account for approximately 75% of the non-carbonate material incorporated into the underlying sediments.In the Arabian Sea the dissolved atmospheric inputs of all the trace metals, with the exception of Cu and Co, exceed those from fluvial run-off by factors which range from 9.6 for Pb to 1.6 for Cr.