Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

Fine structure in a metric type IV burst: Multi-site spectrographic,polarimetric, and heliographic observations

The spectral fine structure of solar radio continua is thought to reveal wave-particle and wave-wave interactions in magnetic traps in the solar corona. We present observations of spectra, polarization, and spatial characteristics of combined emission/extinction features (zebra patterns) during a decimetric/metric type IV event on 5 June, 1990. Very high modulation depths are observed. The size and location of the sources during emission and extinction are determined for the first time. Two remarkable features are found: (1) The sources of emission stripes have finite size, up to nearly 2; during extinction stripes the brightness is reduced across the whole extent of the unperturbed continuum, which is slightly larger than 2. (2) During emission stripes the sources drift over distances up to several × 10⁴ km, with apparent velocities up to 10⁵ km s^–1. The observed features are briefly discussed with respect to interpretations based on wave-particle interactions and on the scattering of electromagnetic waves.     

The Viking biological experiments on Mars

The essential findings of the three biological experiments aboard the two Viking Mars landers are reviewed and compared. All three of the experiments yielded significant data in repeated tests of Martian surface samples. Some of the results are consistent with a biological interpretation, although there are serious reservations in accepting this conclusion. Most of the findings, however, are inconsistent with a biological basis. The combined data suggest the presence of several classes of oxidants on Mars and these would account for the most of the observations. An explanation for the apparent small synthesis of organic matter in the pyrolytic release experiment remains obscure.     

Atmospheric nitrogen inputs into the North Sea: effect on productivity

The ANICE (Atmospheric Nitrogen Inputs into the Coastal Ecosystem) project addressed the atmospheric deposition of nitrogen to the North Sea, with emphasis on coastal effects. ANICE focused on quantifying the deposition of inorganic nitrogen compounds to the North Sea and the governing processes. An overview of the results from modelling and experimental efforts is presented. They serve to identify the role of the atmosphere as a source of biologically essential chemical species to the marine biota. Data from the Weybourne Atmospheric Observatory (UK) are used to evaluate the effect of short episodes with very high atmospheric nitrogen concentrations. One such episode resulted in an average deposition of 0.8 mmol N m⁻² day⁻¹, which has the potential to promote primary productivity of 5.3 mmol C m⁻² day⁻¹. This value is compared to long-term effects determined from model results. The total calculated atmospheric deposition to the North Sea in 1999 is 948 kg N km⁻¹, i.e. 0.19 mmol N m⁻² day⁻¹ which has the potential to promote primary productivity of 1.2 mmol C m⁻² day⁻¹. Detailed results for August 1999 show strong gradients across the North Sea due to adjacent areas where emissions of NO_x and NH₃ are among the highest in Europe. The average atmospheric deposition to the southern part of the North Sea in August 1999 could potentially promote primary production of 2.0 mmol C m⁻² day⁻¹, i.e. 5.5% of the total production at this time of the year in this area of the North Sea. For the entire study area the atmospheric contribution to the primary production per m² is about two-third of this value. Most of the deposition occurs during short periods with high atmospheric concentrations. This atmospheric nitrogen is almost entirely anthropogenic in origin and thus represents a human-induced perturbation of the ecosystem.     

Small-scale vegetation dynamics in semi-arid steppe in Inner Mongolia

Small-scale vegetation dynamics were followed for ecotones and in uniform stands inArtemisia-dominated steppe vegetation under grazing and when recovering from heavy grazing. Species composition was followed annually for 5 years in 1 m²and 0·25 m²plots for (1) presence–absence; (2) density; and (3) biomass.More rapid vegetation dynamics, in terms of change of type of vegetation and distance moved in DCA species space between sampling occasions, were observed in smaller plots and in early seral stages, where species were few and alpha diversity low. Only the plots recently protected from grazing showed a directional vegetation change; those protected for more than 3 years and those under continued grazing showed, at this scale, non-directional dynamics.     

Using Eigenpattern Analysis to Constrain Seasonal Signals in Southern California

— Earthquake fault systems are now thought to be an example of a complex nonlinear system (Bak, et al., 1987; Rundle and Klein, 1995). The spatial and temporal complexity of this system translates into a similar complexity in the surface expression of the underlying physics, including deformation and seismicity. Here we show that a new pattern dynamic methodology can be used to define a unique, finite set of deformation patterns for the Southern California Integrated GPS Network (SCIGN). Similar in nature to the empirical orthogonal functions historically employed in the analysis of atmospheric and oceanographic phenomena (Preisendorfer, 1988), the method derives the eigenvalues and eigenstates from the diagonalization of the correlation matrix using a Karhunen-Loeve expansion (KLE) (Fukunaga, 1970; Rundle et al., 2000; Tiampo et al., 2002). This KLE technique may be used to determine the important modes in both time and space for the southern California GPS data, modes that potentially include such time-dependent signals as plate velocities, viscoelasticity, and seasonal effects. Here we attempt to characterize several of the seasonal vertical signals on various spatial scales. These, in turn, can be used to better model geophysical signals of interest such as coseismic deformation, viscoelastic effects, and creep, as well as provide data assimilation and model verification for large-scale numerical simulations of southern California.     

A Local Example of Land‐Use Change: Southern Illinois—1807, 1938, and 1993*

This article provides an analysis of a wetland site in southern Illinois from presettlement to the present. The study area is part of the Cache River‐Cypress Creek Wetland, which has international importance, as recognized by the Ramsar Convention on Wetlands. Land‐cover data for 1807, 1938, and 1993 were created and analyzed with a geographic information system (GIS). Land‐use change by topographic setting (uplands, transitional, and bottomlands) and soil productivity was quantified and studied. Interviews with local experts informed this analysis. Results illustrate the complexity of environmental change and its driving forces. First, notable forest and swamp acreage was converted to cropland between 1807 and 1938 and, to a lesser degree, from 1938 to 1993. Second, there were land‐use variations by topographic region. Between 1807 and 1938, the largest transformation occurred in the uplands, with substantial acreage converted from forest to cropland. Between 1938 and 1993, however, agriculture decreased in the upland areas as hilly areas reverted to forest cover. At the same time, agriculture expanded in the bottomlands as this land was drained for farming. Third, there are interesting patterns within these categories of land‐use change, as soil productivity is an indicator of what lands were taken out of cropland and converted back to grassland and forest.