The aim of this study was to investigate the effect of nitrate on nitrite toxicity to Microcystis aeruginosa. Short-term uptake experiment revealed nitrate could depress the nitrite active uptake. The long-term experiment of nitrate effects on nitrite toxicity showed a negative relationship between the growth and intracellular nitrite levels of M. aeruginosa. The net nitrite uptake displayed a decline in the range of 0-150 mg NO(3)(-)-NL(-1) and an elevation at 200mg NO(3)(-)-NL(-1), whereas the activities of nitrate reductase (NR) were more sensitive to nitrate than nitrite reductase (NiR). As a result, the total intracellular nitrite levels tended to decrease up to 100 mg NO(3)(-)-NL(-1) then increased at 150-200 mg NO(3)(-)-NL(-1). These results suggested the existence of external nitrate could affect the toxicity of nitrite to alga through changing intracellular nitrite content. 相似文献
We deployed two ADCP mooring systems west of the Luzon Strait in August 2008, and measured the upper ocean currents at high
frequency. Two typhoons passed over the moorings during approximately one-month observation period. Using ADCP observations,
satellite wind and heat flux measurements, and high-resolution model assimilation products, we studied the response of the
upper ocean to typhoons. The first typhoon, Nuri, passed over one of the moorings, resulting in strong Ekman divergence and
significant surface cooling. The cooling of surface water lagged the typhoon wind forcing about one day and lasted about five
days. The second typhoon, Sinlaku, moved northward east of the Luzon Strait, and did not directly impact currents near the
observation regions. Sinlaku increased anomalous surface water transport exchange across the Luzon Strait, which modulated
the surface layer current of the Kuroshio. 相似文献
为了研究碱湖N2O释放速率及其对盐度与pH的响应,选取内蒙古大克泊碱湖的五个沉积物样点,采用15N同位素标记模拟实验,研究反硝化和厌氧氨氧化的速率、相对比例和气体产生情况,揭示高盐和高pH对碱湖氮移除的影响。发现大克泊湖潜在氮移除速率为0~16.06 n mol N mL-1 h-1,潜在反硝化速率为0~12.62 n mol N mL-1 h-1,潜在厌氧氨氧化速率为0~9.81 n mol N mL-1 h-1;当盐度34.00 g·L-1与pH 10.22时,厌氧氨氧化对氮移除贡献较大,达到43.18%~71.79%。反硝化过程气体产物以N2为主,几乎无N2O气体释出。另外,该区域潜在氮移除速率与pH呈正相关关系,与TOC、NO-3、HCO-3呈负相关关系;未发现氮移除速率与盐度之间的相关关系。因此,在研究的碱湖中,氮移除过程中主要为N2排放,而N2O低于检测水平;氮移除过程的影响因素复杂且不限于最主要的环境变量(盐度与pH)。这些结果为研究湖泊N2O排放提供了数据基础。 相似文献
Discrete element method has been widely adopted to simulate processes that are challenging to continuum-based approaches. However, its computational efficiency can be greatly compromised when large number of particles are required to model regions of less interest to researchers. Due to this, the application of DEM to boundary value problems has been limited. This paper introduces a three-dimensional discrete element–finite difference coupling method, in which the discrete–continuum interactions are modeled in local coordinate systems where the force and displacement compatibilities between the coupled subdomains are considered. The method is validated using a model dynamic compaction test on sand. The comparison between the numerical and physical test results shows that the coupling method can effectively simulate the dynamic compaction process. The responses of the DEM model show that dynamic stress propagation (compaction mechanism) and tamper penetration (bearing capacity mechanism) play very different roles in soil deformations. Under impact loading, the soil undergoes a transient weakening process induced by dynamic stress propagation, which makes the soil easier to densify under bearing capacity mechanism. The distribution of tamping energy between the two mechanisms can influence the compaction efficiency, and allocating higher compaction energy to bearing capacity mechanism could improve the efficiency of dynamic compaction.