南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

Relationships between body weight and other morphological traits in young sea cucumbers Apostichopus japonicas

Correlation, multiple regression, and path analyses were used to investigate the relationships between body weight and three other morphological traits in juvenile Japanese sea cucumbers Apostichopus japonicus . We measured live body weight (BW), body length (BL), numbers of papillae (NP), and numbers of tube feet (NF) at 60, 80, 100, and 130 days post-hatching (dph). We calculated path correlation coefficients, correlation indices ( R 2 ), and coefficients of determination with BW as the dependent variable and the other morphological traits as independent variables. The coefficient of variation for BW was high across all age groups, and all measured morphological traits were significantly correlated ( P < 0.01). BL had the greatest direct eff ect on BW across all age groups (60 dph, 0.526;80 dph, 0.404;100 dph, 0.620;and 130 dph, 0.681), while NF had the greatest indirect eff ect on BW across all age groups (60 dph, 0.528;80 dph, 0.452;100 dph, 0.666;and 130 dph, 0.603). Regression analyses between morphological traits and BW indicated that R 2 was greater than 0.85 only in the 100-dph specimens. The indirect eff ects of the other measured morphological traits on BW were age-dependent. The optimal regression equations, as determined with stepwise regression, were, for 60-dph specimens: BW 60 =10 (-3.04+0.092BL+0.014NP+0.014NF)( R 2 =0.632);for 80-dph specimens: BW 80 =10 (- 3.035+0.056BL+0.017NP+0.02NF)( R 2 =0.686);for 100-dph specimens: BW 100 =10 (- 3.742+0.069BL+0. 633 · lg (NP)+0. 464 · lg (NF))( R 2 =0.893);and for 130-dph specimens: BW 130 =10 (- 2.472+0.065BL+0.012NP)( R 2 =0.774). Our work clarified the correlation between various morphological traits and body weight of a commercially-important sea cucumber species ( A . japonicus ). Our predictive models for body weight might be useful for the aquaculture and selective breeding of A . japonicus . These models might also provide theoretical support for the indirect selection of traits that are difficult to select directly.     

CCD astrometric observations of 2017 VR12, Camillo and Midas

We have observed three near-Earth objects(NEOs), 2017 VR12, Camillo and Midas, during 2018.The observations were made with the 1-m telescope, operated by Yunnan Observatories, over two nights.Their precise astrometric positions are derived from 989 CCD observations. The theoretical positions of asteroids are retrieved from the Jet Propulsion Laboratory(JPL) Horizons System and Institut de M′ecanique C′eleste et de Calcul des ′Eph′em′erides(IMCCE). The positions of three asteroids are measured with respect to stars in the Gaia DR2 star catalog. For 2017 VR12, the means(O-C) of right ascension and declination are -0.090′′and-0.623′′respectively based on the published JPL ephemeris, but the corresponding means(O-C) are 3.122′′and-0.636′′based on the published IMCCE ephemeris. The great difference in declination could be explained by several factors.(1) The degraded CCD images caused by the fast apparent motion of the objects lead to a reduction in positioning accuracy.(2) The poor timing system may introduce systematic errors, especially in the high speed direction.(3) The asteroid may be perturbed by Earth when it approaches the Earth too closely. These astrometric results demonstrate that the centroid centering method can reduce the dispersion of non-Gaussian images as compared with the PSF modeling method.For Camillo and Midas, the astrometric results are consistent based on the two ephemerides. Implementing a high-precision timing system, and analyzing some astronomical effects and geometric distortions in CCD images should be carefully considered in future works.     

Unusual infrared emission toward Sgr B2: possible planar C_(24)

Interstellar graphene could be present in the interstellar medium(ISM), resulting from the photochemical processing of polycyclic aromatic hydrocarbon(PAH) molecules and/or collisional fragmentation of graphitic particles. Indeed, by comparing the observed ultraviolet(UV) extinction and infrared(IR) emission of the diffuse ISM with that predicted for graphene, as much as ～ 2% of total interstellar carbon could be locked up in graphene without violating the observational constraints. While the possible detection of planar C24, a small piece of a graphene sheet, has been reported towards several Galactic and extragalactic planetary nebulae, graphene has not yet been detected in interstellar environments. In this work, we search for the characteristic IR features of C24 at ～ 6.6, 9.8 and 20 μm toward Sgr B2, a high-mass star formation region, and find a candidate target toward R.A.(J2000) = 267.05855?and Decl.(J2000) =-28.01479?in Sgr B2 whose Spitzer/IRS spectra exhibit three bands peaking at ～ 6.637, 9.853 and 20.050 μm which appear to be coincident with those of C24. Possible features of C60 are also seen in this region. The candidate region is a warm dust environment heated by massive stars or star clusters, associated with a WISE spot(a tracer of star formation activity), close to the HII region candidate IRAS 17450–2759, and is surrounded by seven young stellar object candidates within ～ 5′, suggesting that the creation and/or excitation of C24 could be related to star formation activities.     

海洋环境对猎雷声呐探测识别的影响及对策建议

猎雷是各海军强国重点发展方向,猎雷作战中首要的就是探测识别水雷。从传播损失、混响、 目标强度以及海流、潮汐等多个方面分析了海洋环境对猎雷声呐探测识别的影响,并从猎雷作战角度提出了有关参数设置、作战方式、数据处理等对策建议,可为部队探测识别水雷等目标提供有效参考。     

航行体近水面滑跳运动试验研究

航行体近水面滑跳运动是近年来跨介质航行体研究的热点问题之一,针对航行体水面滑跳运动现象开展研究。 首先设计了适宜滑跳的航行体构型,通过数值仿真方法确定航行体的主要技术参数,发现航行体滑跳过程对于重心位置非常敏感;然后,针对设计的构型,开展水面自由滑跳试验,试验表明本文设计的模型可以在近水面稳定滑跳,验证了滑跳运动方式的可行性。 工作可为后续跨介质航行体设计提供技术参考。     

无人海洋测绘技术体系构建

随着无人技术的快速发展,无人测绘的时代即将到来。本文分析了无人时代技术特征与海洋测绘发展机遇;讨论了无人海洋测绘新理念,构建了无人海洋测绘技术体系,论述了无人海洋测绘技术关键技术;展望了无人海洋测绘技术发展趋势,及其在海洋经济、海洋可持续发展中的应用前景。     

GNSS接收机DCB短期时变特征分析和估计方法

提出一种使用非差非组合精密单点定位(PPP)估计和分析接收机DCB短时时变特征的方法。首先利用非差非组合PPP得到包含接收机DCB的重构电离层参数估值;然后通过IGS电离层GIMs格网模型内插剥离各历元站星斜向电离层距离延迟;最后通过最小二乘约束得到各历元接收机DCB解。由于格网本身精度(2~8 TECU)和插值精度限制,解算出来的接收机DCB并不能真实反映其短期时变特征。为此,提出利用站间单差或者历元间差分的方法还原其真实的变化态势。实验结果表明,所提出的方法能够正确估计接收机DCB,并能真实还原其短期时变特征,具有良好的适用性。     

Building a model-based personalised recommendation approach for tourist attractions from geotagged social media data

When travelling, people are accustomed to taking and uploading photos on social media websites, which has led to the accumulation of huge numbers of geotagged photos. Combined with multisource information (e.g. weather, transportation, or textual information), these geotagged photos could help us in constructing user preference profiles at a high level of detail. Therefore, using these geotagged photos, we built a personalised recommendation system to provide attraction recommendations that match a user's preferences. Specifically, we retrieved a geotagged photo collection from the public API for Flickr (Flickr.com) and fetched a large amount of other contextual information to rebuild a user's travel history. We then created a model-based recommendation method with a two-stage architecture that consists of candidate generation (the matching process) and candidate ranking. In the matching process, we used a support vector machine model that was modified for multiclass classification to generate the candidate list. In addition, we used a gradient boosting regression tree to score each candidate and rerank the list. Finally, we evaluated our recommendation results with respect to accuracy and ranking ability. Compared with widely used memory-based methods, our proposed method performs significantly better in the cold-start situation and when mining ‘long-tail’ data.