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61.
培养基对海藻真菌PT2菌株的生长及活性代谢产物合成的影响 总被引:3,自引:0,他引:3
本文采用PD、GPY、YES、SYE、GPM5种不同培养基培养海藻真菌菌株PT2,并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析,旨在了解不同培养基对菌株生长和活性物质合成的影响.结果显示,PT2菌株在不同培养基上的培养特征及生长速率有所不同,液体培养基发酵液抽提物的抗肿瘤活性也有较大差别,其中SYE发酵液抽提物活性最强,对KB、Raji和Hela 3种肿瘤细胞的IC50分别为7.6μg/cm^3、2.4μg/cm^3和3.3μg/cm^3.采用HPLC法对不同抽提物进行分析,发现它们之间的组成成分有显著差异.本文的实验结果表明,不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响。 相似文献
62.
Spatial and temporal variability of heat content above the thermocline in the tropical Pacific Ocean 总被引:1,自引:0,他引:1
Abstract-Heat content of the upper layer above the 20℃ isotherm in the tropical Pacific Ocean isestimated by using the sea temperature data set with a resolution 2°latitude×5°longitude (1980~1993)for the water depths (every 10 m) from 0 m to 400 m, and its temporal and spatial variabilities are an-alyzed. (1) The temporal variability indicates that the total heat in the upper layer of the equatorial Pa-cific Ocean is charcterized by the interannual variability. The time series of the equatorial heat anomaly5 months lead that of the El Nino index at the best positive lag correlation between the two, and theformer 13 months lag behind the latter at their best negative lag correlation. Therefore the equatorialheat content can be used as a better predictor than the El Nino index for a warm or cold event. In addi-tion, it is also found that less heat anomaly in the equator corresponds to the stronger warm events inthe period (1980~1993) and much more heat was accumulated in the 4 years including 1992/1 相似文献
63.
本文利用收集到的实测双峰谱型海浪过程资料,把这些资料以波高和周期的相关系数为参数分成5组,讨论每组双峰谱型下被高、周期的统计分布.并探讨了相关系数对波高分布和周期分布的影响。 相似文献
64.
本文以粤西水东湾表层沉积物样品的粒度分布为基本数据,使用对应分析方法并结合地形与波流特征,分析了海湾动力沉积环境。分析结果表明,海湾现代沉积环境可划分为4个动力沉积区。 相似文献
65.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
66.
本文分析了雷达观测结果,发现近海海面上的水汽向上输送随季节而变化,由于季节的变化和不同的天气情况,海表面大气现象在雷达中有不同的显示,根据这些显示得到了不同的信息,由此而得出春秋两季海表面水汽向上输送量的不同。 相似文献
67.
沉积物对磷酸盐的吸附与释放 总被引:3,自引:0,他引:3
本实验研究黄河口水文站沉积物对黄河水磷的吸附释放过程和东海111号站位沉积物对海水磷的吸附释放过程。并摸拟黄河沉积物在海水中磷的吸附释放过程。结果表明:底沉积物对磷的吸附相当迅速,无论是河泥还是海泥在24h内均能达到吸附平衡。吸附量与沉积物的量和水中原有磷的浓度有关,在不同的pH条件下,沉积物对磷的吸附是有显著差别的 相似文献
68.
本文介绍了厦门岛市区机动车流遥感监测的实施目的和方法,通过航空影像有关题信息解译与实例、专题图件编制以及统计资料的对比分析,认为:目前厦门岛市区交通主要存在着机动车增长过速、道路狭窄和会计室场少且分布不合理等三个方面的问题,文中提出了相应的建议。体现了航空遥感对于中、大比例尺城市动态监测快速有效的特点。 相似文献
69.
The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC50 for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species. 相似文献
70.