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Inorganic lead (Pb) is one of the most common environmental pollutants. Much evidence indicates that Pb exposure could directly affect fish growth and development. In this study, we investigated the cytotoxic effects of Pb on cytoskeletal protein stability at both protein and mRNA level in crucian carp Carassius auratus. Pb(NO3)2 treatment in concentration of 100 μmol/L resulted in decreased expression of both α- and β-tubulin but γ-tubulin as assayed with SDS-PAGE, Western Blot, and ELISA. In vivo and in vitro analyses on protein expression of tubulins are consistent. The effect of Pb on mRNA expression varied among different tissues. Our results suggest that cytotoxicity of Pb at protein translation level is stronger than at mRNA expression level. 相似文献
905.
Water quality assessment for Ulansuhai Lake using fuzzy clustering and pattern recognition 总被引:1,自引:0,他引:1
Water quality assessment of lakes is important to determine functional zones of water use. Considering the fuzziness during the partitioning process for lake water quality in an arid area, a multiplex model of fuzzy clustering with pattern recognition was developed by integrating transitive closure method, ISODATA algorithm in fuzzy clustering and fuzzy pattern recognition. The model was applied to partition the Ulansuhai Lake, a typical shallow lake in arid climate zone in the west part of Inner Mongolia, China and grade the condition of water quality divisions. The results showed that the partition well matched the real conditions of the lake, and the method has been proved accurate in the application. 相似文献
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On the basis of artificial neural network (ANN) model, this paper presents an algorithm for inversing snow depth with use of AMSR-E (Advanced Microwave Scanning Radiometer-Earth Observing System (EOS)) dataset, i.e., brightness temperature at 18.7 and 36.5GHz in Qinghai-Tibet Plateau during the snow season of 2002-2003. In order to overcome the overfitting problem in ANN modeling, this methodology adopts a Bayesian regularization approach. The experiments are performed to compare the results obtained from the ANN-based algorithm with those obtained from other existing algorithms, i.e., Chang algorithm, spectral polarization difference (SPD) algorithm, and temperature gradient (TG) algorithm. The experimental results show that the presented algorithm has the highest accuracy in estimating snow depth. In addition, the effects of the noises in datasets on model fitting can be decreased due to adopting the Bayesian regularization approach. 相似文献
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现行黄河口水下三角洲海底形貌及不稳定性 总被引:4,自引:0,他引:4
杨作升 G.H.Keller 陆念祖 D.B.Prior 林天充 B.D.Bornhold 许卫东 L.D.Wright J.Suhayda 曹立华 W.J.Wisenman 《中国海洋大学学报(自然科学版)》1990,(1)
高分辨力的声学仪器调查表明,现行黄河口水下三角洲海底的形貌十分复杂,由于高浓度巨量黄河泥沙快速沉积的结果,使河口底坡出现了大量的不稳定性现象,如塌陷冲沟,高密度沉积物重力流,切割—充填结构海底,V形水下河谷、滑塌等。根据海底扰动程度作了分区,划分了地形单元,并对不同的海底形态的成因作了分析。比较了1985和1986年两个航次考查结果的变动,分析了原因。 相似文献