3株海洋来源微生物代谢产物及其抗肿瘤活性

从3株海洋微生物发酵物的抗肿瘤活性部位分离鉴定了放线菌内酯素(1)、2-吡咯酸(2)、5,7,4'-三羟基异黄酮(3)、7,4'-二羟基异黄酮(4)、环(脯-甘)二肽(5)、N-甲酰基-星形孢菌素(6)、过氧化麦角甾醇(7)、6,9-环氧麦角甾-7,22-二烯-3-醇(8)等8个化合物。这些化合物分别产自2株放线菌L39-3(1和2)、L20-1d1(3~6)和1株真菌LJW-110(7和8)。抗肿瘤活性初步测试结果表明,除2和5以外对K562细胞均有不同程度抑制作用,在100μg/mL浓度下的抑制率约在32%~66%范围之内。     

陆地与海洋来源放线菌次级代谢能力的核糖体工程改造

以3株抗肿瘤活性新化合物产生菌放线菌野生株YN17707(陆地)、18522(陆地)和007(海洋)为出发菌,用核糖体工程抗生素抗性筛选技术,改造次级产物代谢生产能力。通过链霉素抗性筛选,得到突变株212株,经发酵产物的TLC和HPLC分析,高产原始菌所产化合物的突变株87株(41%),可产新产物的突变株22株(10%)。在18522的108株突变株中,高产突变株达53.7%(58株),产新产物突变株达11.1%(12株),链霉素抗性筛选对18522的效果较为理想。     

2株无抗肿瘤活性放线菌野生株的抗生素抗性突变株及其抗肿瘤活性筛选

以2株放线菌无活性野生株M15(海洋)和M13(陆地)为原始菌,利用核糖体工程抗性筛选技术,通过对M15的链霉素、新霉素、利福平、庆大霉素抗性筛选,得到抗性突变株共63株,经对M13的链霉素抗性筛选,得到链霉素抗性突变株18株。其中,6株M15突变株在活性测试中示有抗肿瘤活性,100μg/mL样品对K562细胞的抑制率大于30%,活性最强的1株新霉素8μg/mL抗性突变株CTM154-8N抑制率高达93.3%,而从M13突变株中没有发现活性突变株。     

两郧断裂郧县盆地段地质与地球物理特征研究

通过野外地质调查,结合地球物理探测手段,研究两郧断裂郧县盆地段地质与地球物理特征;参照两郧断裂带多个位置采集断层物质的SEM、TL测试成果,并结合本次对断层上断点沉积物OSL测年数据,对两郧断裂该段第四纪活动性进行分析。研究结果表明,两郧断裂在郧县盆地段埋深相对较深;断裂特征主要为逆断层,部分分支断裂显示为正断性质;断裂带最新活动时代为中更新世晚期-晚更新世早期。     

一种顾及有色噪声的四星座GNSS动态导航滤波算法

经典Kalman滤波要求噪声是高斯白噪声,而动态GNSS定位的观测误差和状态预测误差往往是有色噪声。本文提出一种简便的有色噪声函数模型拟合滤波算法,采用前面历元的观测残差和状态残差建立有色噪声模型,削弱有色噪声对动态导航解算的影响。采用四星座GNSS接收机观测数据进行动态导航实验,结果表明,顾及有色噪声的动态导航滤波算法比未顾及有色噪声的经典Kalman滤波算法定位精度更高,三维位置精度提升9%以上。     

东营市矿产资源开发利用现状与保护措施

以东营市矿产资源总体规划为指导,进行矿产资源开发利用与保护措施研究,在开发利用现状的基础上分析当前存在的矿产资源后备基地紧缺、综合利用水平不高以及过度开采等问题,进而提出促进矿产资源开发利用与保护的措施,以便更好地为东营市经济建设和社会发展服务。     

Adaptive Predictive Inverse Control of Offshore Jacket Platform Based on Rough Neural Network

The offshore jacket platform is a complex and time-varying nonlinear system,which can be excited of harmful vibration by external loads.It is difficult to obtain an ideal control performance for passive control methods or traditional active control methods based on accurate mathematic model.In this paper,an adaptive inverse control method is proposed on the basis of novel rough neural networks (RNN) to control the harmful vibration of the offshore jacket platform,and the offshore jacket platform model is established by dynamic stiffness matrix (DSM) method.Benefited from the nonlinear processing ability of the neural networks and data interpretation ability of the rough set theory,RNN is utilized to identify the predictive inverse model of the offshore jacket platform system.Then the identified model is used as the adaptive predictive inverse controller to control the harmful vibration caused by wave and wind loads,and to deal with the delay problem caused by signal transmission in the control process.The numerical results show that the constructed novel RNN has advantages such as clear structure,fast training speed and strong error-tolerance ability,and the proposed method based on RNN can effectively control the harmfid vibration of the offshore jacket platform.     

Comparison of the effects of short-term UVB radiation exposure on phytoplankton photosynthesis in the temperate Changjiang and subtropical Zhujiang estuaries of China

We examined the effect of solar ultraviolet radiation B (UVB, 280–315 nm) on photosynthesis of natural phytoplankton assemblages in the temperate Changjiang River Estuary (CRE) in the East China Sea (ECS), and the subtropical Zhujiang River Estuary (ZRE) in the South China Sea (SCS) from August 2002 to April 2003. The short-term effect of UVB was assessed by exposing samples in quartz tubes/bottles to solar radiation under three treatments: (1) natural sunlight (NS) with UVB (NS-UVB); (2) photosynthetically active radiation (PAR, NS cut off UVB); and (3) NS with additional artificial UVB (NS + A-UVB). Solar UVB apparently inhibited phytoplankton photosynthesis rates. In the temperate CRE-ECS, solar UVB reduced surface phytoplankton photosynthesis by about 28% in August and February, while in the subtropical ZRE-SCS the inhibition was only 22% in September and October. In the CRE-ECS, phytoplankton in the stratified water column displayed stronger UVB inhibition when deeper water samples were exposed to surface UVB. Phytoplankton in the mixed water column did not show strong UVB inhibition, while light shift exposure of deeper phytoplankton in the same water column to surface light produced similar results, indicating that mixing moderates UVB effects. In the ZRE-SCS, surface phytoplankton showed greater photoinhibition in January (sunny). However, in April (cloudy), phytoplankton showed little UVB inhibition. Incubation for a short time without UVB showed a large increase in Chl a at two stations in the ZRE-SCS, but a large decrease at the other station in the presence of UVB. In contrast, in the CRE-ECS, a similar incubation experiment without UVB showed a decrease in Chl a, and small UVB inhibition of Chl a at two stations. Nutrient conditions might have played a role in the difference of UVB inhibition between the two regions as the ZRESCS had relatively high concentrations of all nutrients while PO₄ was only 0.21 μM at one of the CRE-ECS stations. The results suggest that phytoplankton in temperate waters would be more responsive to variation of UVB than ones in subtropical waters.     

海洋红藻多管藻内生真菌EN-22 的化学成分研究

对采自广西涠州岛近海多管藻(Poiysiphonia urceolata)分离到的一株内生真菌EN-22的次生代谢产物的化学成分进行了研究.利用正相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析、制备薄层层析(PTLC)以及重结晶等分离手段从该菌发酵液中分离得到15个化合物,通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构,分别为:4-(4-喹啉)-4-羟基-2-丁酮(1),3-羟基-3-(2-氧丙基)吲哚-2-酮(2),3-吲哚乙醇(3),3-吲哚甲酸(4),2-羟基-3-吲哚丙酸(5),2-酮-3-吲哚乙醇(6),3,3-二吲哚-2-羟基-丙醇(7),β-咔啉(8),尿嘧啶(9),环-(S-脯氨酸-S-异亮氨酸)(10),(22E,24R)-3β,5α,9α-三羟基麦角甾-7,22-二烯-6-酮(11),(22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7α-二醇(12),5α,6α-环氧-24(R)-甲基胆甾-7,22-二烯-3β-醇(13),胡萝卜苷(14),过氧化麦角甾醇(15);其中化合物1和2为新天然产物,并首次报道化合物1的碳谱数据.     

三疣梭子蟹溞状Ⅰ期幼体的饥饿实验

通过观察不同饥饿胁迫处理对三疣梭子蟹(Portunus trituberculatus)溞状Ⅰ期幼体变态和发育的影响,研究了其"营养储存饱和点"(the point of reserve saturation,PRS)和"不可恢复点"(the point of no return,PNR)现象的发生情况.结果表明:以轮虫为饵料,三疣梭子蟹溞状Ⅰ期幼体(ZⅠ)在前期投喂后期饥饿的条件下,随着前期摄食时间的增加,幼体变态率和发育速度呈上升趋势,50%营养储存饱和点(PRS50)出现在47.5 h,100%营养储存饱和点(PRS100)出现在72 h;在前期饥饿后期投喂的条件下,随着前期饥饿时间的增加,幼体变态率和发育速度呈下降趋势,50%不可恢复点(PNR50)出现在39 h,100%不可恢复点(PNR100)出现在56 h.