Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

Photosynthetic response of floating Ulva prolifera to diurnal changes of in-situ environments on the sea surface

During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local environments. The purpose of this study is to investigate the response of the thalli to diurnal changes of environments on the sea surface, and a sea surface environment simulator (SSES) was designed to simulate the natural environment of floating U . prolifera mat. A control experiment is designed in the laboratory, which was processed under suitable conditions (20°C for temperature, 72 μmol photons/(m 2 ·s) for light intensity and 30 for salinity), and an in-situ research was conducted to study the photosynthetic responses of floating U . prolifera to diurnal changes of environments. The results show that the photosynthetic efficiency of the thalli decreased gradually with time, and decreased rapidly after 14:00 local time (LT). After exposed to the environment on the sea surface for 6 h, the photosynthetic activity of the thalli decreased significantly. Furthermore, physiological-level and molecular-level experiments revealed that non-photochemical quenching (NPQ), cyclic electron flow (CEF) and energy redistribution between PSI and PSII all played an important role in the strong photosynthetic plasticity of U . prolifera . NPQ is the most important photoprotective responses to environmental changes before 12:00 LT. The results also confirmed that the CEF and energy redistribution between PSI and PSII are the main synergistic eff ects for the thalli to adapt to the environmental changes when the process NPQ cannot work. And the result can further reveal the reason why U . prolifera can adapt to the living condition of long distance drift on the sea surface. The findings of this research could provide a theoretical basis for explaining outbreaks of the green tide and instructing the management of the problem.     

秦岭地区NDVI与地形因子的相关性分析

秦岭山区地形因子是影响植被分布的重要因素。选取2001、2009和2017年MODIS陆地产品MOD13Q1数据和DEM数据,从DEM中提取地形因子,高程、坡度和坡向,与MODIS的NDVI数据结合,分析了地形对秦岭地区植被空间分布影响。研究结果表明:(1)NDVI随着高程的增大而逐渐增大,在高程1800 m左右时达到最大值,随后又随着高程的增大而减小;(2)NDVI在坡度0°～5°间逐渐增大,在5°～40°呈稳定趋势,从40°开始缓慢减小,60°达到乔木能够生长的坡面倾角临界值,当坡面倾角大于60°时植被指数开始快速减小;(3)受太阳辐射的影响,坡向在NW 270°～360°,SE 240°～270°之间的植被长势较好,其余坡向上长势一般。     

PDR算法对地磁室内定位精度的提升研究

利用建筑物中金属结构引起的地磁场扰动可以对室内的行人目标进行定位,而且基于地磁场的定位无需布设任何额外设施,因此可以以低成本实现定位。但仅靠单一的地磁技术无法满足室内定位的精度要求。为了解决磁场数据中单点定位的模糊性问题,本文提出了一种利用粒子滤波算法将PDR与地磁相融合的室内定位方法,并开发了地磁室内导航系统,以智能手机为硬件平台构建磁力计传感器模型,建立匹配轨迹的均方误差准则并实现PDR累积误差实时校正的迭代计算。在68 m×1.8 m的试验区域内,产生的平均定位误差为1.13 m,最大定位误差为2.17 m。本文算法的定位精度比单独PDR算法提升了42%;与单一地磁指纹匹配算法相比,定位精度提高了57%。试验证明,本文提出的融合算法对提高室内定位精度具有显著的作用。     

检索词优化与空间自适应的深网POI获取方法研究

针对检索词库构建困难、数据请求量受限制等相关问题,该文提出一种基于独立覆盖率排序和空间自适应剖分的深网POI信息搜索方法,通过候选检索词初步构建、贪婪式探测搜索、检索词优化降维、空间自适应剖分爬行等主要流程,利用逐步逼近POI搜索的最大覆盖最优解,实现对深网POI信息的全量获取,大幅度提高深网POI数据的召回率与采集效率,该方法对于丰富地理信息资源、提升空间信息服务与内容管理能力具有重要意义。     

机载WIDAS数据的Landsat卫星反照率初步验证

随着精细化监测的需求,中高空间分辨率的地表反照率产品逐渐成为气候模型的主要输入。目前,中高空间分辨率反照率产品的验证主要基于地表站点的通量塔观测数据,区域机载飞行数据的验证依然相对较少。因此,本文基于区域机载数据验证Landsat反照率产品。针对内蒙古自治区根河森林试验区所获取的机载红外广角双模式成像仪(WIDAS)多角度反射率数据,应用BRDF原型反演算法估算其反照率,分析了应用机载数据验证中高空间分辨率反照率产品的潜力。2016年内蒙古根河森林试验区机载WIDAS飞行多角度观测的可用多角度范围为25°,以前的研究表明BRDF原型反照率反演算法表现出对小观测角度的反照率反演结果的鲁棒性。因此,机载WIDAS反照率在一定程度可用于星载反照率的验证。首先,基于核驱动模型和各向异性平整指数(AFX)提取了试验区4种MODIS二向性反射分布函数(BRDF)原型;然后,将其作为先验知识应用到根河森林WIDAS机载数据的反照率反演中;最后,用WIDAS反照率和单个地面通量塔观测的反照率对Landsat卫星数据的反照率进行初步验证。验证结果表明Landsat反照率与WIDAS反照率结果较为一致,但略有低估,总体均方根误差(RMSE)约为0.02,偏差为0.0057。在多角度观测范围较小时,BRDF原型的反照率反演算法可为星载地表反照率的验证提供了一种有效的验证手段。     

顾及周期性误差修正的加权平均温度模型构建

针对目前多数大气加权平均温度(Tm)模型没有考虑季节性影响这一问题,该文首先利用IGRA 2005-2010年全球探空数据,分别建立了各探空站点与地表温度有关的线性Tm模型、与地表水汽压有关的指数Tm模型以及与地表温度和水汽压均有关的混合Tm模型。然后以探空站积分Tm值为参考,对上述3类模型的误差时间序列进行了分析,发现这3种模型均存在周期性误差,并在此基础上构建了考虑周期性误差修正的3类Tm新模型。利用2011-2014年全球探空数据对3类新模型进行精度验证,结果表明:3类Tm新模型的精度相比于原模型均有所提升,模型的周期性误差影响基本得以消除,且3类Tm新模型的精度基本一致。     

RTK无验潮水深测量误差分析及控制方法

实时动态(real time kinematic,RTK)无验潮水深测量技术已成为海洋测绘发展的方向。从RTK无验潮水深测量原理和误差来源入手,定性与定量分析了高程拟合误差、波浪引起的误差、仪器测量误差、定位时延以及仪器安装等误差对成果精度的影响,指出选择恰当的拟合模型是控制高程拟合误差的关键,安装姿态传感器可以削弱波浪引起的误差,提出测量前对换能器连接杆进行垂直度测量、采用双频接收机和PPS等控制方法可以有效减小测量误差。结合实践提出减小RTK无验潮水深测量精度四点建议,为RTK无验潮水深测量技术的推广应用提供参考。     

基于AI的胸部CT平扫对冠脉钙化积分的准确性评价

目的:探讨基于人工智能(AI)的非门控胸部低剂量CT(LDCT)平扫与心电门控CT血管造影(ECG gated-CTA)对冠状动脉钙化积分(CACS)危险度分层的一致性.方法:回顾性分析行冠状动脉CTA扫描的100例患者,所有患者均行冠脉ECG gated-CTA扫描和常规非门控胸部LDCT平扫.在Siemens后处理...     

Rapid Urbanization Induced Extensive Forest Loss to Urban Land in the Guangdong-Hong Kong-Macao Greater Bay Area,China

China has experienced rapid urbanizations with dramatic land cover changes since 1978. Forest loss is one of land cover changes, and it induces various eco-environmental degradation issues. As one of China’s hotspot regions, the Guangdong-Hong KongMacao Greater Bay Area(GBA) has undergone a dramatic urban expansion. To better understand forest dynamics and protect forest ecosystem, revealing the processes, patterns and underlying drivers of forest loss is essential. This study focused on the spatiotemporal evolution and potential driving factors of forest loss in the GBA at regional and city level. The Landsat time-series images from 1987 to2017 were used to derive forest, and landscape metrics and geographic information system(GIS) were applied to implement further spatial analysis. The results showed that: 1) 14.86% of the total urban growth area of the GBA was obtained from the forest loss in1987–2017;meanwhile, the forest loss area of the GBA reached 4040.6 km2, of which 25.60%(1034.42 km2) was converted to urban land;2) the percentages of forest loss to urban land in Dongguan(19.14%), Guangzhou(18.35%) and Shenzhen(15.81%) were higher than those in other cities;3) the forest became increasingly fragmented from 1987–2007, and then the fragmentation decreased from2007 to 2017);4) the landscape responses to forest changes varied with the scale;and 5) some forest loss to urban regions moved from low-elevation and gentle-slope terrains to higher-elevation and steep-slope terrains over time, especially in Shenzhen and Hong Kong.Urbanization and industrialization greatly drove forest loss and fragmentation, and, notably, hillside urban land expansion may have contributed to hillside forest loss. The findings will help policy makers in maintaining the stability of forest ecosystems, and provide some new insights into forest management and conservation.