深部开采底板隔水关键层受局部高承压水作用破坏理论分析

大量实测地质资料与防治水实践证实,深部煤层开采底板含水层存在大量的局部高承压水区域,在强开采扰动下极易诱发底板突水灾害。基于“下三带”、“关键层”理论,将局部高承压水与底板隔水关键层简化为圆筒力学模型,分析研究隔水关键层在局部高承压水作用下的屈服破坏机制,得到了隔水关键层发生屈服的4个临界水压值公式;对各临界水压值进行区间划分,分析不同区间隔水关键层的屈服状态,确定了局部高承压水致使隔水关键层屈服破坏两个阶段的水压判据平衡方程,得出了奥陶系顶部岩层作为隔水关键层剩余隔水能力的计算表达式,并反演得到了开采深度上限、下限公式。结合九龙矿深部开采实际,对所建力学模型进行了验证与应用,进而提出了防止局部高承压水致灾的相关措施,对实现深部煤层安全带压开采具有重要的理论指导意义。     

深海热液区管状蠕虫Ridgeia piscesae β型半乳糖凝集素的克隆与功能研究

Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp c DNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10–50°C and p H 5–10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards He La cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.     

凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析

为研究凯氏拟小球藻(Parachlorellakessleri)糖代谢分子机制,本研究采用RT-PCR和RACE技术从凯氏拟小球藻中克隆了己糖激酶基因CkeHK(GenBankID:AHF54566),并对其自养、异养、混养条件下的转录表达进行分析。结果表明,该序列的cDNA全长为1844bp,开放阅读框1389bp,编码462个氨基酸。该蛋白的相对分子质量为49.73,等电点为6.98。实时荧光定量PCR结果显示,以自养培养条件为对照,异养培养和混养培养条件下,CkeHK均能够发生明显上调,且混养条件下上调量比异养条件下上调量更多,说明CkeHK可能在凯氏拟小球藻利用外源糖的过程发挥重要作用,并且光信号对于凯氏拟小球藻利用外源糖可能存在调控作用。这些研究结果为进一步阐明CkeHK的功能及其作用机制奠定了分子基础。     

海底边界层溶解氧通量原位观测技术方法研究

海底边界层是溶质和悬浮颗粒物在海床与水体间交换的重要场所,也是沉积物质的主要成岩作用区,对海洋、湖泊有着重要的生物、化学、地质学意义。底栖溶解氧消耗速率是评估早期碳矿化作用的重要指示剂,也是研究元素循环周转的重要指标。溶解氧通量原位监测技术是获取底栖溶解氧消耗速率、了解化学物质输运机制,进而研究底栖生态系统、环境污染影响、生物地球化学进程的重要手段。针对传统溶解氧观测方法在效率和准确度上的局限性,介绍了目前底边界层原位氧通量测量技术的发展现状,对包括底栖培养室、微电极剖面、涡动相关技术和平面光极系统在内的观测特点进行了对比分析,讨论了各自的不足与局限,提出了今后需要关注的改进方向。     

Third-Order Stokes Wave Solutions of the Free Surface Capillary-Gravity Wave and the Interfacial Internal Wave

Based on the Stokes wave theory, the capillary-gravity wave and the interfacial internal wave in two-layer constant depth''s fluid system are investigated. The fluids are assumed to be incompressible, inviscid and irrotational. The third-order Stokes wave solutions are given by using a perturbation method. The results indicate that the third-order solutions depend on the surface tension, the density and the depth of each layer. As expected, the first-order solutions are the linear theoretical results (the small amplitude wave theoretical results). The second-order and the third-order solutions describe the nonlinear modification and the nonlinear interactions. The nonlinear impact appears not only in the n (n≥2) times'' high frequency components, but also in the low frequency components. It is also noted that the wave velocity depends on the wave number, depth, wave amplitude and surface tension.     

SAR与光学影像融合增强方法和效果分析

基于Radarsat-2全极化SAR、GF-1多光谱、Landsat-8多光谱影像,利用PCA变换、HSV变换等方法,分析了同空间分辨率的SAR与光学全色影像的信息量,并评价了其分别与Landsat-8多光谱影像的融合结果。结果表明:全极化SAR影像较全色影像的亮度高,图像标准差、信息熵及平均梯度总体趋于一致,信息量相当;融合影像目视效果相近,且SAR融合影像较光学融合影像包含的信息量更为丰富,利用高分全极化SAR影像替代光学全色影像开展海岸带遥感监测是可行的。     

滇西北云岭陆缘弧大地构造单元的划分与特征

为进一步查明滇西北云岭陆缘弧的地质构造特征,经实地调研与资料收集整理,将该区划分为6个四级大地构造单元.即：雪龙山岩群（Pt₁Xl）、德钦断块（Pz₁）、攀天阁酸性火山岩浆弧（T₂）、工农弧间盆地（T₂）、崔依比中基性火山岩浆弧（T_1-2）、鲁甸火山弧-同碰撞花岗岩（T）.前2个单元属岛弧基底,后4个单元属岛弧主体.各单元的岩石组合、变形变质特征有明显差异.岛弧主体形成于早三叠世-晚三叠世早期,是金沙江洋盆向西俯冲消减、碰撞造山的产物.岛弧主体之上被上三叠统角度不整合覆盖.     

滇西南澜沧江带红豆山铜矿花岗斑岩地质地球化学特征及成岩时代

南澜沧江火山弧带红豆山花岗斑岩属于过铝质钾玄岩性S型花岗岩,SiO_2含量为71.1%~74.4%,全碱含量高(K_2O+Na_2O=8.4%~9.4%),富钾(K_2O/Na_2O=3.9~5.0),铝饱和指数(A/CNK)为1.06~1.21;具轻稀土元素富集特征,负Eu异常(δ Eu=0.53~0.74);相对富集大离子亲石元素(LILE,如Rb、Ba、Th、U等),但亏损Sr、P、Ti等元素。地球化学特征表明,红豆山花岗斑岩具火山弧花岗岩与同碰撞花岗岩的特征,为活动大陆边缘弧的产物。红豆山花岗斑岩的LA-ICP-MS锆石U-Pb年龄为220.3±3.6Ma,形成于晚三叠世。红豆山花岗斑岩的地球化学特征和形成时代与小定西组基性火山岩非常相似,两者是同一期岩浆活动不同阶段的产物。     

基于逆重复m序列信号的岩矿石电性参数采集方法研究

归纳总结了岩矿石电性参数实验过程中激励场源性质与实验装置类型,提出基于伪随机逆重复m序列信号为激励场源的实验方案,进而设计了新的观测装置.发送机产生的伪随机信号序列采用恒流源形式输出,频带宽度为1.0×10-3～1.0×104 Hz;接收机采用FGPA+ARM架构进行数据记录,在对比变频法、2n 伪随机序列与逆重复m序...     

北斗高精度无人机航测技术在农村土地承包经营权确权项目中的应用

在农村土地承包经营权确权项目中,当地政府部门提供的地方影像图大都比例尺较大,详细程度较低,无法作为工作底图。为获取比例尺合适的影像图,无人机低空航测摄影技术已成为目前最重要的测绘手段。无人机航测技术可进行分辨率为0.05m的全覆盖航拍,本文选取地面控制点进行了正射纠正,提高了影像的几何精度,增强了可解译性,并制作了现势性强、精度高且定位准确的1:1000比例尺的正射影像图。