Effect of carbon and nitrogen ratio control on Artemia growth,water quality,biofloc microbial diversity under high salinity and zero-water exchange culture condition

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.     

Impacts of Coastal Reclamation on Natural Wetlands in Large River Deltas in China

Little information is available on the impacts of coastal reclamation on wetland loss in large-river deltas at a regional scale.Using remote sensing data of coastal wetland and reclamation in four deltas in China from 1978 to 2014, we tracked their continuous area changes in four periods: 1978–1990, 1990–2000, 2000–2008, and 2008–2014. The areal relation between wetland loss and reclamation was quantified and used to identify coastal reclamation mode intensity coupled with another three indicators: reclamation rate,accretion rate and land-use intensity of coastal reclamation. The results showed that coastal reclamation driven by economic development reduced, or even reverse the original growth of delta which was determined by the offset between wetland acceleration rate and wetland loss rate. Generally, the area of reclamation showed a positive linear correlation with the area of wetland loss. The findings imply that human activities should control reclamation rate and intensity to alleviate total wetland loss and maintain wetland ’net gain’.Inappropriate coastal reclamation modes can magnify total wetland loss;therefore, coastal reclamation with a slow increment rate and low impervious surface percent is of great importance for sustainable development in future coastal management.     

深圳市浴场用海定级研究

海域分等定级是制定海域使用金征收标准和开展海域资产价格评估的前提和依据。借鉴《海域定级技术指引(试行)》,遵循资源禀赋差异、收益差异、节约利用和生态用海的原则选取海域自然条件和海域区位条件2个定级因素及海水质量、海洋自然灾害等10个评价因子,构建深圳市浴场用海定级的评价指标体系。采用综合指数评价法计算出207个评价单元的得分情况,通过聚类分析方法和变异系数(CV)来校核级别,最终将浴场用海的海域划分为Ⅰ级(22.22%)、Ⅱ级(33.82%)和Ⅲ级(43.96%)3个级别。研究成果为海洋管理部门制定浴场用海不同级别的基准价格提供了依据。     

养殖裙带菜机械损伤防御应答的初步研究

以裙带菜(Undariapinnatifida(Harv.)Suringar)幼孢子体为材料,测定藻体对机械损伤的防御应答。结果显示:与对照组相比,机械损伤会诱导藻体内活性氧含量上升,以及抗氧化酶活性和抗氧化物含量的变化。当裙带菜受到机械损伤后,体内O2-和H2O2以及MDA等物质迅速积累,藻体的总抗氧化能力上升、CAT和POD活性在短时间内迅速升高、GSH和ASA迅速合成,从而在较短时间内控制活性氧以及MDA含量;与对照组相比,SOD活性仅在3h内高于对照组。该实验结果表明裙带菜幼孢子遭受机械损伤后的抗氧化系统会参与调节机体活性氧平衡,这种抗氧化机制的存在可以更快速、更节能、更有效地修复藻体所受损伤。     

航空重力垂直梯度探测潜艇方法研究

航空重力梯度测量属于被动探测,抗干扰能力强,如果能和其他探潜手段相配合将极大地提高航空搜潜的效率。针对航空重力梯度测量是否能够用于探测潜艇的问题,依据俄亥俄级弹道导弹核潜艇的结构特点,建立了适用于重力梯度计算的潜艇模型,分别给出了潜艇外壳、内部质量亏损产生的重力垂直梯度的计算方法,并对不同精度重力梯度仪可探测的潜艇重力垂直梯度值进行了计算,从航空反潜的角度对探测潜艇效果进行了分析。经计算表明,如航空重力梯度仪精度达到10~(-2) E,将具备一定的实际探潜效能;如精度达到10~(-4) E,反潜机搜索宽度可与现有航空磁性探潜相当。     

海底玄武岩中次生组分HCl溶解实验研究

次生组分沉淀是海底岩石中普遍存在的现象,次生组分去除是减少次生组分干扰、获取海底岩石高质量数据的关键。选择西太平洋麦哲伦海山区MK海山相对新鲜的玄武岩作为研究对象,经研磨、均一化后将粉末样品分为49份,其中48份分别以0.25,0.50,1.00,1.50,2.00,2.50 mol/L六种浓度的足量HCl溶解0.5,1,2,4,8,16,32和64 h后,选取溶解残渣测定主要氧化物和特征微量元素质量分数,确定HCl溶解法在去除次生组分的同时不会对海底新鲜玄武岩中原生组分造成影响的HCl浓度-时间组合。结果表明,在HCl浓度为1.00～2.50 mol/L,溶解时间为0.5～2 h的12种HCl浓度-时间组合均可保证海底玄武岩中原生组分的安全,并可有效去除次生组分。综合海底蚀变玄武岩次生组分去除实验研究,推荐使用1.00～2.00 mol/L HCl,溶解时间在0.5～1 h的实验方案。     

机载WIDAS数据的Landsat卫星反照率初步验证

随着精细化监测的需求,中高空间分辨率的地表反照率产品逐渐成为气候模型的主要输入。目前,中高空间分辨率反照率产品的验证主要基于地表站点的通量塔观测数据,区域机载飞行数据的验证依然相对较少。因此,本文基于区域机载数据验证Landsat反照率产品。针对内蒙古自治区根河森林试验区所获取的机载红外广角双模式成像仪(WIDAS)多角度反射率数据,应用BRDF原型反演算法估算其反照率,分析了应用机载数据验证中高空间分辨率反照率产品的潜力。2016年内蒙古根河森林试验区机载WIDAS飞行多角度观测的可用多角度范围为25°,以前的研究表明BRDF原型反照率反演算法表现出对小观测角度的反照率反演结果的鲁棒性。因此,机载WIDAS反照率在一定程度可用于星载反照率的验证。首先,基于核驱动模型和各向异性平整指数(AFX)提取了试验区4种MODIS二向性反射分布函数(BRDF)原型;然后,将其作为先验知识应用到根河森林WIDAS机载数据的反照率反演中;最后,用WIDAS反照率和单个地面通量塔观测的反照率对Landsat卫星数据的反照率进行初步验证。验证结果表明Landsat反照率与WIDAS反照率结果较为一致,但略有低估,总体均方根误差(RMSE)约为0.02,偏差为0.0057。在多角度观测范围较小时,BRDF原型的反照率反演算法可为星载地表反照率的验证提供了一种有效的验证手段。     

检索词优化与空间自适应的深网POI获取方法研究

针对检索词库构建困难、数据请求量受限制等相关问题,该文提出一种基于独立覆盖率排序和空间自适应剖分的深网POI信息搜索方法,通过候选检索词初步构建、贪婪式探测搜索、检索词优化降维、空间自适应剖分爬行等主要流程,利用逐步逼近POI搜索的最大覆盖最优解,实现对深网POI信息的全量获取,大幅度提高深网POI数据的召回率与采集效率,该方法对于丰富地理信息资源、提升空间信息服务与内容管理能力具有重要意义。     

顾及周期性误差修正的加权平均温度模型构建

针对目前多数大气加权平均温度(Tm)模型没有考虑季节性影响这一问题,该文首先利用IGRA 2005-2010年全球探空数据,分别建立了各探空站点与地表温度有关的线性Tm模型、与地表水汽压有关的指数Tm模型以及与地表温度和水汽压均有关的混合Tm模型。然后以探空站积分Tm值为参考,对上述3类模型的误差时间序列进行了分析,发现这3种模型均存在周期性误差,并在此基础上构建了考虑周期性误差修正的3类Tm新模型。利用2011-2014年全球探空数据对3类新模型进行精度验证,结果表明:3类Tm新模型的精度相比于原模型均有所提升,模型的周期性误差影响基本得以消除,且3类Tm新模型的精度基本一致。