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141.
提要应用RNeasy animal mini试剂盒抽提大黄鱼total RNA,逆转录合成模板cDNA;同时综合分析从GenBank数据库查询到已报道的转铁蛋白(Tf)基因序列,先设计并合成扩增引物扩增出大黄鱼转铁蛋白部分基因序列,再应用RACE技术,克隆出大黄鱼转铁蛋白全长cDNA基因,全长2461个核苷酸,编码661个氨基酸。应用WCG软件包,采用DNADIST和NEIGHBOR分析方法,构建了转铁蛋白系统进化树。树图显示与传统的分类地位大致相符,可以看出转铁蛋白在海水鱼和淡水鱼的进化上的差异,同时也显示大黄鱼Tf与真鲷的同源性最高。 相似文献
142.
The VOF method and the k–ε model, combined with the equation of state of air at constant temperature, have been used to calculate the total horizontal wave force caused by monochromatic waves acting on a perforated caisson with a top cover. From comparison of various parameters, such as the total horizontal force, the pressure difference on the front wall, the pressure on the back wall and the pressure on the top cover, between the numerical results and test data, it can be seen that the numerical results agree well with the test data. It is concluded that the method described in this paper can be utilized to calculate wave forces acting on perforated caissons with a top cover in the case of nonovertopping, nonbreaking waves. A simplified method to calculate the total horizontal force has been developed, based on test data, using a least-squares method. A comparison between the numerical results and the values calculated from the simplified equations shows good agreement. Therefore the simplified equations can be used in engineering applications to evaluate the total horizontal force on a perforated caisson with a top cover. 相似文献
143.
144.
牙鲆(Paralichthys olivaceus)是我国北方沿海地区的重要经济养殖品种,目前已形成工厂化生产规模。随着养殖密度的增加以及海水污染程度的日益严重,养殖牙鲆出现了各种疾病(曲凌云等,2000; Mo Zhaolan et al.,2001).造成了巨大的经济损失。
1999~2000年,山东省荣成、威海、青岛胶南及黄岛地区养殖牙鲆大面积发病,疾病发生时间多在每年的6~11月.病鱼多为3~10月龄。本文作者对以上牙鲆发病地区进行了临床病症的调查,并进行了病原的分离和初步鉴定。 相似文献
145.
146.
闽南近海尖头斜齿鲨的生殖生物学研究 总被引:12,自引:0,他引:12
尖头斜齿鲨(Scoliodon laticaudus)是暖水性小型鲨鱼,为有胎盘胎生种类.在闽南近海,其雄鱼的性成熟长度为382~448mm,雌鱼的性成熟长度为405~467mm,1994年5月至1995年4月的周年随机采样中,雌鱼在群体中所占比例为53.0%.雌鱼的妊娠开始于1~4月,同年的6~8月分娩,妊娠期为5~6个月.尖头斜齿鲨雌鱼每个子宫一次妊娠怀胚胎1~12尾,其中3~6尾者占67.8%.雌鱼一次妊娠产仔鱼2~24尾,平均9.1尾,其中6~13尾占70%;57%的母体左右子宫内胚胎数相同.胚胎数量随母体全长的增大而呈线性增加.雌性尖头斜齿鲨生殖周期约为2年,一生生殖2~3次,生殖能力较低.尖头斜齿鲨胚胎在分娩时全长138~142mm,与雌鱼性成熟长度的比为0.30~0.35,胚胎的雌雄比为1.17:1.同一子宫内及左右两子宫中的胚胎发育情况均基本相同.另外还讨论了鲨鱼资源的保护和管理问题. 相似文献
147.
Tan Jiahua Ma Wei Pan Bin Associate Professor Shanghai Jiao Tong University Shanghai Lecturer shanghai Jiao Tong University Shanghai 《中国海洋工程》1992,(1)
The basis, process and results of the demonstration of the main dimensions of a 75000t floating production and storage vessel are discussed in this paper. A simple but reliable orthogonal design method is applied in the main dimension optimization. The ideas of gradual approximation and feedback from various aspects are put into effect. During the demonstration, in order to make the model tally with the actual situation, the draft design is closely related to the computational analysis, so that the demonstration model can be verified at any time; the handling of the overall system is closely related to the research of each item, which is beneficial not only to the mastery of various regularities, but also to the balance of decisions. Finally, according to the computational results and the regularities obtained from analysis, the main dimensions are determined. 相似文献
148.
DYNAMIC ANALYSIS OF TYPHOON SURGES ALONG THE COASTS OF ZHEJIANG AND JIANGSU PROVINCES 总被引:1,自引:0,他引:1
Based on the numerical model shown in Ref. [1], a dynamic analysis is given for the storm surges caused by the four typhoons (i. e. typhoons Nos. 5310, 5612, 6126 and 6207) on the east coast of Zhejiang and Jiangsu Provinces, in which the contributions of various dynamic factors to local surge elevation are calculated numerically, including the Coriolis force, the atmospheric pressure force, the bottom friction, the local topography and the couple nonlinear interactions between surge current and elevation as well as between surge currents themselves. Moreover, the responses of these factors to different kinds of typhoon tracks and water depths are discussed in more detail, and the effects of grid size on the computed results demonstrated. 相似文献
149.
In seven cruises on benthic organisms in the nearshore waters(>50 m) to the west of the Taiwan Strait(23°20''-25°54''N,117°11''-119°58''E) in 1961-1964,altogether 189 stations of quantitative grabbing and 33 stations of qualitative trawling were made.
Altogether 392 species of benthic organisms have been identified.The composition was dominated by nearshore shallow water species,with tropical and subtropical species absolutely dominant.The total average of biomass was 38.0 g/m2 and average density,116.0 ind./m2.The communities of benthic organisms in the area can be classified into five communities namely,(1) Amphiura sp.-Acaudina molpadioides Community;(2) Protankyra asymmetrica-Arnphioplus pracstans-Murex trapa Community;(3) Modiolus meicalfei-Phyllophorus liuwutiensis-Armandia letocirris-Nephtys sinensis Community;(4) Branchiostoma belcheri-Solenr oseomaculatus-Marphysa sinensis- Onuphis eremita Community;(5) Iconomtra japonica-Barbatia decussata Community. 相似文献
Altogether 392 species of benthic organisms have been identified.The composition was dominated by nearshore shallow water species,with tropical and subtropical species absolutely dominant.The total average of biomass was 38.0 g/m2 and average density,116.0 ind./m2.The communities of benthic organisms in the area can be classified into five communities namely,(1) Amphiura sp.-Acaudina molpadioides Community;(2) Protankyra asymmetrica-Arnphioplus pracstans-Murex trapa Community;(3) Modiolus meicalfei-Phyllophorus liuwutiensis-Armandia letocirris-Nephtys sinensis Community;(4) Branchiostoma belcheri-Solenr oseomaculatus-Marphysa sinensis- Onuphis eremita Community;(5) Iconomtra japonica-Barbatia decussata Community. 相似文献
150.