西藏地温的年际和年代际变化

利用1971-2005 年西藏10 个站的0.8 m、1.6 m 和3.2 m 逐月平均地温资料,采用气候倾向率等现代统计诊断方法,研究了近35 年西藏年、季平均地温的变化趋势、气候突变和异 常年份。结果表明：0.8 m 年平均地温在西藏东部的林芝、昌都呈现为下降趋势,其他各站以0.19~0.81 ^oC/10a 的速率升高;有5 个站的1.6 m 年平均地温呈显著的升高趋势,升温率为 0.20~0.60 ^oC/10a;3.2 m 年平均地温6 个站均表现为升高趋势,为0.13~0.52 ^oC/10a,以拉萨升温率最大。在0.8 m 处,① 大部分站点季平均地温呈明显的上升趋势,其中西藏西部、南 部以夏季升幅最大,特别是狮泉河达1.61 ^oC/10a;北部以冬季增温最突出。东部地区四分之三的季平均地温呈降温趋势。② 大部分站点年平均地温呈逐年代升高趋势,而昌都表现为逐年代降低趋势。③ 狮泉河春、夏季平均地温分别在1996 年和1983 年发生了气候突变;拉萨和日喀则年、季平均地温发生的气候突变是从一个相对偏冷期跃变为一个相对偏暖期,前者 出现在20 世纪80 年代,后者发生在20 世纪90 年代初;而林芝1993 年夏、秋季出现的气候 突变是从一个相对偏暖期跃变为一个相对偏冷期。④ 西藏西部年、季平均地温以异常偏高年份居多,且发生在20 世纪末至21 世纪前5 年;南部年、季平均地温均为异常偏高年份,主要出现在20 世纪90 年代中后期;北部年、季平均地温异常偏高年均出现在21 世纪前5 年, 异常偏低年份以20 世纪80 年代居多;东部年平均地温以异常偏低年为主。青藏铁路沿线西藏境内测站最大冻土深度以-4.5~-25.4 cm/10a 的速率呈显著减小趋势,安多减幅最大     

地理试卷效度求证——运用因子分析法进行单维性检验

效度是地理试卷质量分析的重要指标之一。根据评估方法的不同,效度可分为内容效度、结构效度和效标关联效度三种类型。其中,结构效度是效度概念的核心,其计算多使用因子分析法,目的是验证测试的单维性。这是因为测试只有在单维性的前提下,才能使用测量理论(包括经典测量理论、概化理论和项目反应理论)对考试进行质量分析。本文以嘉兴市某次高三模拟考为例,探讨结构效度的计算和分析,并进行测试单维性的检验,确保之后教育测量理论的运用。     

西藏麦地卡湿地的浮游植物——1.优势种的时空生态位

为了解青藏高原湿地浮游植物在时空不同维度上的分布特征,本文选取西藏四大国际湿地之一的麦地卡湿地作为研究地点,于2019年7月和10月在麦地卡湿地主要水系——麦地藏布及其支流和湖泊,设置了14个样点,采集了2个水情期（丰水期和枯水期）浮游植物的水样共252个.通过活体观察和固定染色方法鉴定浮游藻类物种,使用直接计数法统计浮游藻类的数量.计算麦地卡湿地浮游植物优势种的优势度指数（Y）、更替率（r）、生态位宽度（B_i）、生态位重叠值（O_ik）和生态响应速率（R）,运用生态位分化的方法,对麦地卡湿地浮游植物优势种的生态位进行了不同时空维度的研究.结果表明：本研究中鉴定出浮游植物优势种（Y>0.02）共8种,其中克洛脆杆藻（Fragilaria crotomensis）和窗格平板藻（Tabellaria fenestrata）是2个水情期的共有优势种.生态位宽度值差异较大,可分为三大生态位类群,且优势度指数与生态位宽度值呈极显著正相关.总体上浮游植物优势物种的生态位重叠值高,在时间、空间和时空三个维度上O_ik>0.6的分别占89.29%、60.71%和28.57%.生态响应速率之和在时间维度上为负数,在空间和时空维度上为正数,说明该湿地浮游植物群落处于发展阶段.冗余分析表明,酸碱度、溶解氧、总氮和浊度是影响麦地卡湿地浮游植物优势种分布的重要因素,浮游植物优势种在这些环境因子上存在明显的生态分化现象.     

西藏拉鲁湿地浮游植物群落时空分布特征及其驱动因子

为了解西藏拉鲁湿地浮游植物群落时空分布特征及其驱动因子,于2021年7月、10月和2022年5月在拉鲁湿地主要水系进行定性和定量样品采集,共采集浮游植物水样126个,从不同季节和不同生境浮游植物种类组成、多样性等方面入手,探讨拉鲁湿地浮游植物群落时空分布特征及其驱动因子,为研究拉鲁湿地生态系统和稳定发展提供基础资料。结果表明:1)拉鲁湿地共鉴定出浮游植物532种(含变种和变型),隶属于8门13纲36目74科130属,物种组成表现为硅藻-绿藻-蓝藻型。2)拉鲁湿地浮游植物群落特征参数在不同季节和不同生境之间存在差异。夏季浮游植物群落的Shannon-Wiener多样性指数显著高于秋季和春季,Simpson优势度指数显著高于秋季。秋季浮游植物群落的Pielou均匀度指数显著低于夏季和春季。春季浮游植物群落的Margalef丰富度指数显著低于夏季和秋季。沼泽生境的浮游植物群落的Margalef丰富度指数显著高于湿草甸生境。3)季节是浮游植物群落组成差异的关键因素,生境对浮游植物群落组成的影响较弱,夏季的距离衰减模式比秋季和春季更强。4)拉鲁湿地不同的浮游植物群落参数与不同环境因子之间存在显著的关联。5)空间因素在解释浮游植物群落变异方面具有显著贡献,而快速变化的水质变量在决定浮游植物的季节变化中起着重要作用。季节和缓慢变化水质因子的解释能力相对较弱,但仍对浮游植物群落的变异有一定影响。     

粉土及粉质黏土对静压沉桩桩端阻力影响机制现场试验

为探讨在粉土及粉质黏土中桩端阻力随贯入深度的变化规律,通过在试验桩P1的桩端安装轮辐压力传感器,以及在试验桩P1、P2距桩端200 mm处安装光纤光栅（FBG）传感器,采用两种不同的测试技术全程监测了两根闭口预应力高强度混凝土（PHC）管桩现场贯入过程中的桩端阻力。试验结果表明：桩端阻力与土层的变化密切相关,土层越硬,桩端阻力越大,当桩端从粉质黏土层进入粉土层时,桩端阻力明显增大,粉土中的桩端阻力达到粉质黏土层的2倍;整个贯入过程中,同一土层不同位置的差异性对桩端阻力也存在较大影响,在距离地面1.50 m处,P1和P2桩FBG传感器桩端阻力的差值达到了89.29 kN,而在距离地面3.50~4.50 m处,两桩的桩端阻力则相差较小。     

Spatial variations of small phytoplankton contributions in the Northern Bering Sea and the Southern Chukchi Sea

Phytoplankton size classes (hereafter, PSCs) were derived from satellite ocean color data using a present phytoplankton abundance-based optical algorithm in the northern Bering and southern Chukchi Seas to characterize the spatial and seasonal variations of the different PSC and investigate the contributions of small phytoplankton to the total phytoplankton biomass. The comparison results showed that the phytoplankton abundance-based method approach could reasonably classify the three PSCs (pico-, nano-, and micro phytoplankton). The satellite maps of the dominant PSCs were derived using long-term satellite ocean color data. The general spatial distribution showed that the large (micro-) phytoplankton were dominant in the coastal waters and the west side of the Bering strait, while the small size (nano- or pico-) phytoplankton were dominant in the open ocean waters. Nano- and microphytoplankton were dominant in May and October in most of the study area, while pico-phytoplankton were dominant in the summer months in the open ocean waters. The annual variation in small phytoplankton dominance had a strong positive relationship with the annual mean sea surface temperature (SST), which is consistent with the increasing dominance of small phytoplankton biomass as water temperature increases. Microphytoplankton have an apparent increasing trend in the southeastern Chukchi Sea but slightly decreasing trends in Chirikov and St. Lawrence Island Polynya (SLIP). In contrast, there were increasing trends in picophytoplankton in Chirikov and SLIP, which seems to be related to increasing annual SST. It is crucial to monitor changes in dominant groups of phytoplankton community in the Bering and Chukchi Seas as important biological hotspots responding to the recent changes in environmental conditions.     

一种诱导模式和甘油补料优化策略提高重组大肠杆菌BL21（DE3）β-琼胶酶的产量

琼胶酶是作用于琼胶的水解酶,在食品、化妆品和制药工业中有广泛的应用。本文建立了一种基于诱导模式和甘油补料优化的高细胞密度和高产β-琼胶酶策略,同时可以较好的控制乙酸盐产量。首先,在诱导前期采用不同的比生长速率（μ）的甘油指数补料策略。结果表明,低的比生长速率（μ=0.2）是细胞生长和β-琼胶酶产生的最佳条件。其次,研究了诱导阶段诱导温度和诱导物浓度对细胞生长和β-琼胶酶产生的影响。当异丙基-β-d-硫代半乳糖苷（IPTG）诱导时,在20℃下0.8mmol/L的IPTG诱导策略对β-琼胶酶的产生效果最佳。采用1.0g/（L·h）的乳糖连续补料策略诱导培养,β-琼胶酶活性达到112.5U/mL,是目前报道的产量最高的β-琼胶酶。此外,β-琼胶酶能直接酶解龙须菜粉,产生新琼寡糖,水解产物为新琼胶四糖（NA4）和新琼胶六糖（NA6）。本文的研究为β-琼胶酶的工业化生产和应用提供了较好的理论依据。     

Stipe Length as an Indicator of Reproductive Maturity in the Kelp <Emphasis Type="Italic">Ecklonia cava</Emphasis>

We conducted testing to determine whether stipe length is a useful indicator of reproductive maturity in the kelp Ecklonia cava, a species that plays a pivotal role in ecosystem functioning and services in subtidal areas. Approximately 100 sporophytes with stipes of various lengths were collected during the fertile period (July–November, 2013). We investigated the relationships between stipe length and other morphological characteristics to determine whether stipe length could indicate the age of reproductive maturity. Primary blade length, longest blade length, thallus height, and total length showed significant relationships with stipe length. The length of the primary and longest blades gradually declined as stipe length increased above 125 mm. Zoosporangial sori were found on the blades of more than 70% of individuals with stipes longer than 125 mm, but on only 8% of individuals with stipes less than 125 mm long. Stipe length therefore seems to be an acceptable proxy for reproductive maturity. Another factor to consider, however, is that all specimens with zoosporangial sori, regardless of stipe length, had a dry weight of 80 g or more; thus, individual biomass may also be an important parameter influencing the initiation of reproduction.     

Validation of NCEP and OAFlux air-sea heat fluxes using observations from a Black Pearl wave glider

Latent and sensible heat fluxes based on observations from a Black Pearl wave glider were estimated along the main stream of the Kuroshio Current from the East China Sea to the east coast of Japan, from December 2018 to January 2019. It is found that the data obtained by the wave glider were comparable to the sea surface temperature data from the Operational Sea Surface Temperature and Sea Ice Analysis and the wind field data from WindSat. The Coupled Ocean Atmosphere Response Experiment 3.0 (COARE 3.0) algorithm was used to calculate the change in air-sea turbulent heat flux along the Kuroshio. The averaged latent heat flux (LHF) and sensible heat flux (SHF) were 235 W/m2 and 134 W/m2, respectively, and the values in the Kuroshio were significant larger than those in the East China Sea. The LHF and SHF obtained from Objectively Analyzed Air-Sea Fluxes for the Global Oceans (OAFlux) were closer to those measured by the wave glider than those obtained from National Centers for Environmental Prediction (NCEP) reanalysis products. The maximum deviation occurred in the East China Sea and the recirculation zone of the Kuroshio (deviation of SHF >200 W/m2; deviation of LHF >400 W/m2). This indicates that the NCEP and OAFlux products have large biases in areas with complex circulation. The wave glider has great potential to observe air-sea heat fluxes with a complex circulation structure.     

香港地区海陆地震联测及深部地壳结构研究

为了探明南海北部海陆过渡带的深部地壳结构,我们在香港外海域进行了一次海陆地震联洲的实验,利用固定地震台网远距离接收海上气枪信号,接收距离远达200多km,并利用此次实验的测线1剖面模拟得到了海陆过渡带的深部地壳速度结构.速度结构模型表明:研究区海陆过渡带的地壳结构非均匀性较明显,由陆至海沉积层有一个突然增厚的特点;莫霍面深度约为26～29 km,上地壳P波速度约为5.5～6.4 km/s,下地壳P波速度为6.5～6.9 km/s.在担杆列岛往海方向有一个低速破碎带,其上地壳P波速度为5.2～6.1 km/s,下地壳P波速度为6.2～6.4 km/s,结合野外地质调查的结果,推测它可能为滨海断裂带.在担杆列岛往陆方向香港和深圳之间的研究区域,莫霍面有较大起伏,可能与此处发育的海丰断裂有关.