溶藻弧菌acfA基因克隆与生物信息学分析

溶藻弧菌引起海水养殖产业弧菌病的主要病原,附着定植因子基因acfA是溶藻弧菌重要毒力基因之一,其表达产物是溶藻弧菌有效定植在宿主肠道上所必需的蛋白。根据弧菌属细菌acfA基因基因保守区设计引物,采用Touchdown PCR扩增acfA基因部分序列,Inverse PCR和Nested PCR扩增已知序列侧翼序列,成功克隆了溶藻弧菌acfA基因全长。克隆的acfA基因序列全长为743 bp,开放阅读框长度为648 bp组成,共编码215个氨基酸,在5′端上游未发现-35区和-10区序列。演绎的ACFA蛋白N端有18个氨基酸信号肽序列,表明该蛋白是分泌型蛋白;蛋白结构分析表明主要由α螺旋和不规则卷曲构成。BLAST分析表明,该蛋白氨基酸序列与其他弧菌相应蛋白同源性较高,是较保守的外膜蛋白。     

Urban Economic Cluster Template and Its Dynamics of Beijing,China

Economic clusters have been a central focus of current urban and regional research, policies and practices. However, a meth- odology to identify and analyze policy-relevant economic cluster dynamics is still not well developed. Based on input-output （I-O） data of 1987, 1992, 1997, 2002 and 2007 of Beijing, this article presents an adapted principle component analysis for identifying the evolu- tion of local economic cluster patterns. This research addresses the changes of economic interaction of industries with complementary and common activities over time. The identified clusters provide an insight into the reality of economic development in a diversifying urban economy： the increasing importance of services and the growing interaction between service and manufacturing industries. Our method therefore provides the analysts with a better understanding of the emergence, disappearance and development of economic clus- ters citywide. The results could be used to assist monitoring urban economic development and designing more practical urban economic strategies.     

点态性质与一致性质的一个新关系

为了研究点态性质和一致性质之间的关系,应用紧性性质在拓扑意义下得到逐点有界性质蕴含一致有界性质的结论,并将其推广到连续函数族的情况.基于Baire定理,得到完备度量空间中的逐点收敛性质可导出一致有界性质的结论.     

Distributions and air-sea fluxes of CO2 in the summer Bering Sea

The 3rd Chinese National Arctic Research Expedition(CHINARE–Arctic III) was carried out from July to September in 2008. The partial pressure of CO2(pCO2) in the atmosphere and in surface seawater were determined in the Bering Sea during July 11–27, 2008, and a large number of seawater samples were taken for total alkalinity(TA) and total dissolved inorganic carbon(DIC) analysis. The distributions of CO2 parameters in the Bering Sea and their controlling factors were discussed. The pCO2 values in surface seawater presented a drastic variation from 148 to 563 μatm(1 μatm = 1.013 25×10-1 Pa). The lowest pCO2 values were observed near the Bering Sea shelf break while the highest pCO2 existed at the western Bering Strait. The Bering Sea generally acts as a net sink for atmospheric CO2 in summer. The air-sea CO2 fluxes in the Bering Sea shelf, slope, and basin were estimated at-9.4,-16.3, and-5.1 mmol/(m2·d), respectively. The annual uptake of CO2 was about 34 Tg C in the Bering Sea.     

商业平板式超滤膜包完整性检验：对海洋胶体生物地球化学研究的启示

The performance and integrity of a cassette cross-flow ultrafilter(Pellicon 2, Millipore) are examined with a suite of macromolecules of different molecular masses. The retention coefficient during the cross-flow ultrafiltration experiments increases with increasing molecular mass and reaches 90% with 10 kDa dextran in both milli-Q water and ultrafiltered seawater media. Based on a 90% retention coefficient, the molecular mass cut-off for the ultrafiltration membrane is defined at 10 kDa, which is ten times(1 kDa) that rated by the manufacturer. To further validate the accuracy of the laboratory calibration, the samples from the lower Zhujiang River and the Jiulong River Estuary are ultrafiltered with the cassette ultrafiltration membrane and the colloidal organic carbon abundances in these samples are quantified with the ultrafiltration permeation model based on time series permeation subsamples. The colloidal organic carbon abundances are 5.8%–21.1% in the Jiulong River Estuary and 5.6%–11.0% in the lower Zhujiang River. These are consistent with the reported values for both estuaries as well as with the colloidal organic carbon abundances in marine environments over the coastal and open oceans with 10 kDa cut-off membranes. Therefore, these field data support the laboratory calibration result and indicate the validity of the experimental and quantification procedure adopted. The discrepancy between the nominal molecular mass cut-off and the actual pore size of the ultrafiltration membrane should be of great concern for research in colloidal and nanoparticle biogeochemistry. Careful examination of the membrane integrity should be taken during ultrafiltration experiments in order to avoid misleading molecular mass cut-off information.     

坛紫菜（Porphyra haitanensis）丝状藻体生长增殖的优化调控培养条件研究

丝状藻体是紫菜生活史中的重要阶段,其生长增殖与环境条件密切相关.本研究考察了与坛紫菜丝状藻体生长增殖相关的多个影响因子,探讨了可促进坛紫菜丝状藻体生长、达到生长速率快的优化调控培养条件.具体操作实施过程为：初始丝状藻体来自成熟紫菜叶状体的果孢子释放,基于8因子3水平的正交实验设计方案[采用正交表L27（313）],确定了丝状藻体生长对多个环境因子的响应以及优化的调控培养条件.结果表明,除开pH、复合维生素浓度和NaHCO3浓度外,其他因子对丝状藻体生长均有显著的影响（p≤0.043 8）.获得的优化调控培养条件为：温度18℃、光强27μmol/m2·s-1、盐度25、pH值8.5、复合维生素浓度15μg/dm3、NaHCO3浓度2 mg/dm3、每天光照时数18 h、营养液浓度为f培养介质.优化调控培养条件下的丝状藻体生长速率高达12.07%,平均值±标准偏差SD为（10.70±0.88）%/d.     

黔西南泥堡金矿床构造特征及其控矿作用

位于扬子板块西南缘的右江盆地是我国卡林型金矿的集中分布区之一,素有滇-黔-桂"金三角"之称.泥堡金矿是右江盆地西北侧一个大型矿床,矿区构造主要有NEE向泥堡背斜、二龙抢宝背斜和同方向的F1、F3、F4、F5和F9断层,以及产于F1断层下盘的层间破碎带等.详细构造调查和新近勘查资料的综合研究表明:F1、F5和F9为逆冲断...     

GIS软件在油气长输管道项目矿（产）权确认中的应用

通过在西气东输三线西段（二标段）管道工程项目运用,很好的解决了对矿（产）区、集体用地的矿（产）权确认,为管道经过矿（产）权区确定合理的补偿金额提供科学谈判依据;有效减少了补偿损失;为今后在长输管道穿越矿（产）区、集体用地的补偿提供了测算手段和方法,形成的补偿协议对管道运营单位确定管道权益和维护使用也具有现实意义。     

结合地形因子与分层策略的喀斯特地区无人机影像分类

西南喀斯特山区地形起伏较大,地物分布较为破碎,致使传统的光谱特征一次分类方法的精度较低。本文基于高分辨率无人机正射影像和地形指标,充分利用无人机遥感影像空间特征、光谱特征、纹理特征及地形特征,采取面向对象CART决策树算法与分层策略提取了研究区土地覆盖类型。研究表明,结合空间地形因子和分层策略的方法减少了破碎区地物间的相干扰,故具有较高的分类精度,总体分类精度达91.2%,Kappa系数为0.87,较传统一次分类精度提高了9.8%,Kappa系数提高了0.13。该方法对西南喀斯特地区土地覆盖解译精度较好,可为土地利用监测提供参考。     

基于GPS垂向位移测定云南地区陆地水储量及其对累积降雨的响应机制

本文基于中国大陆构造环境监测网（陆态网）GPS台站垂向形变数据,通过主成分分析计算云南省6大流域陆地水负荷形变,并与降雨数据进行相关性分析和交叉小波分析。结果表明,云南省各流域陆地水储量变化相对于降雨在半年和年周期上分别存在1.5~2.25和3~6个月的滞后,不同周期滞后的叠加使陆地水储量与累积降雨量之间存在显著的负相关性,6大流域的降雨累积天数各不相同,均在47~109 d之间。本文由此提出一种云南地区陆地水储量对累积降雨的响应机制。