2012—2014年舟山近岸海域海洋功能区水质达标率统计与评价

随着国家对长江流域实施环境综合整治、浙江省委省政府开展“五水共治”等环境整治工程,陆源入海污染物总量总体呈下降趋势,近几年舟山近岸海域环境质量呈好转态势。2007—2013年舟山近岸海域水质均不符合第一类海水水质标准,2014年符合第一类海水水质标准的海域有1 269 km2,仅占全市海域面积的6.1%,海水质量总体形势不容乐观,水体富营养化程度较高,海洋功能区水质达标率较低。通过对舟山海域84个二级类基本功能区水质达标率进行统计分析,2012—2014年海洋功能区水质达标率分别为35.7%、38.1%和44.0%,超标因子为无机氮和活性磷酸盐。     

基于锦州湾围填海的海岸线演变分析

自20世纪70年代以来,锦州湾附近海域实施大量填海造陆工程,在促进经济发展的同时也对生态环境造成不良影响,有必要对锦州湾附近海域的围填海状况进行连续监测。文章以5个时相的Landsat TM遥感影像作为主要数据,利用RS和GIS技术手段进行锦州湾附近海域海岸线的提取,系统分析锦州湾围填海海岸线的演进过程,并从海岸线长度和海岸线曲折度等方面对锦州湾围填海开发特点进行定量分析。     

长岛县南五岛土壤地球化学特征及环境质量评价

以长岛县南五岛土壤为研究对象,对区内土壤元素的含量特征进行分析,并对土壤环境质量进行评价。结果表明：研究区土壤平均含量与烟台市、山东省以及全国土壤平均水平相比,除CaO,Na2O,Hg,S,Se,P,F元素含量差异较明显,其他大部分元素含量相对稳定,元素含量的高低不仅与研究区背景条件有关,也与人类活动密切相关。研究区土壤环境质量整体较好,大部分区域属于清洁区和尚清洁区,仅城区局部受到一定程度的Cd,Hg,Zn污染。     

阳朔“西街现象”的形成机理分析——旅游地发展中的游客理性消费与政府“无为而治”

阳朔县作为中国最早对外开放旅游的县之一,其演化历程具有独特性和典型性。在旅游参与期曾出现过一段主客关系和谐、商业秩序与休闲氛围俱佳的“西街现象”,引起媒体和业界的广泛关注,尚缺乏学术层面的解读。本文采用实证归纳的案例研究方法,借鉴旅游治理和目的地发展的相关理论,分析了“西街现象”的形成过程与内在机理,得出以下结论和启示：在旅游发展初期,信息完全的理性旅游消费者的到来,有助于规范目的地的商业经营环境。市场与社区自下而上互动协商达成共识的秩序,促成激励诚信经营的非正式制度嵌入于人际网络和旅游交易的场域之中,政府顺应市场规律即能实现“无为而治”的良性发展状态。本文通过归纳以有预算的理性游客作为市场推动力所触发的旅游地的发展特点,部分回应消费转型背景下旅游市场理性回归后的可能影响。对现实的启发是,适当控制旅游发展的速度和规模,给予本地人成长的时间和空间,教育游客与提升政府监管力度双管齐下,建立规则在先,更利于目的地实现可持续性的发展。     

Effect of benzo[a]pyrene on detoxification and the activity of antioxidant enzymes of marine microalgae

The objective of this study was to examine the effect of benzo[a]pyrene(Ba P) on the detoxification and antioxidant systems of two microalgae,Isochrysis zhanjiangensis and Platymonas subcordiformis.In our study,these two algae were exposed to Ba P for 4 days at three different concentrations including 0.5 μg~(L-1)(low),3 μg~(L-1)(mid) and 18 μg~(L-1)(high).The activity of detoxification enzymes,ethoxyresorufin O-deethylase(EROD) and glutathione S-transferase(GST) increased in P.subcordiformis in all Ba P-treated groups.In I.zhanjiangensis,the activity of these two enzymes increased at the beginning of exposure,and then decreased in the groups treated with mid-and high Ba P.The activity of antioxidant enzyme superoxide dismutase(SOD) increased in I.zhanjiangensis in all Ba P-treated groups,and then decreased in high Ba P-treated group,while no significant change was observed in P.subcordiformis.The activity of antioxidant enzyme catalase(CAT) increased in I.zhanjiangensis and P.subcordiformis in all Ba Ptreated groups.The content of malondialdehyde(MDA) in Isochrysis zhanjiangensis increased first,and then decreased in high Ba P-treated group,while no change occurred in P.subcordiformis.These results demonstrated that Ba P significantly influenced the activity of detoxifying and antioxidant enzymes in microalgae.The metabolic related enzymes(EROD,GST and CAT) may serve as sensitive biomarkers of measuring the contamination level of Ba P in marine water.     

Depth classification of underwater targets based on complex acoustic intensity of normal modes

In order to solve the problem of depth classification of the underwater target in a very low frequency acoustic field, the active component of cross spectra of particle pressure and horizontal velocity (ACCSPPHV) is adopted to distinguish the surface vessel and the underwater target. According to the effective depth of a Pekeris waveguide, the placing depth forecasting equations of passive vertical double vector hydrophones are proposed. Numerical examples show that when the sum of depths of two hydrophones is the effective depth, the sign distribution of ACCSPPHV has nothing to do with horizontal distance; in addition, the sum of the first critical surface and the second critical surface is equal to the effective depth. By setting the first critical surface less than the difference between the effective water depth and the actual water depth, that is, the second critical surface is greater than the actual depth, the three positive and negative regions of the whole ocean volume are equivalent to two positive and negative regions and therefore the depth classification of the underwater target is obtained. Besides, when the 20 m water depth is taken as the first critical surface in the simulation of underwater targets (40 Hz, 50 Hz, and 60 Hz respectively), the effectiveness of the algorithm and the correctness of relevant conclusions are verified, and the analysis of the corresponding forecasting performance is conducted.     

Numerical Studies on Piled Gravity Base Foundation for Offshore Wind Turbine

This study aims to investigate a hybrid gravity base foundation to support offshore wind tower. A new hybrid gravity base foundation considered in this study has five component piles, referred to as ‘piled gravity base foundation’. The three-dimensional finite element analyses were carried out for the piled gravity base foundation subjected to a combined load with a lateral load and overturning moment. The parametric analyses were undertaken varying the loading height and direction, the rigidity of the piled gravity base foundation, the field soil layers, and the clay strength. Overall, the response of the piled gravity base foundation was significantly influenced by the interaction between the cone base piles and the surrounding soil. The increased strength of the soil led to a significant reduction of the pile and gravity base foundation responses, in terms of the bending moments, axial forces, lateral displacements, and rotations.     

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.     

Theoretical calculation of equilibrium Mg isotope fractionation between silicate melt and its vapor

Isotope fractionation during the evaporation of silicate melt and condensation of vapor has been widely used to explain various isotope signals observed in lunar soils, cosmic spherules, calcium–aluminum-rich inclusions, and bulk compositions of planetary materials. During evaporation and condensation, the equilibrium isotope fractionation factor (α) between high-temperature silicate melt and vapor is a fundamental parameter that can constrain the melt’s isotopic compositions. However, equilibrium α is difficult to calibrate experimentally. Here we used Mg as an example and calculated equilibrium Mg isotope fractionation in MgSiO₃ and Mg₂SiO₄ melt–vapor systems based on first-principles molecular dynamics and the high-temperature approximation of the Bigeleisen–Mayer equation. We found that, at 2500 K, δ²⁵Mg values in the MgSiO₃ and Mg₂SiO₄ melts were 0.141?±?0.004 and 0.143?±?0.003‰ more positive than in their respective vapors. The corresponding δ²⁶Mg values were 0.270?±?0.008 and 0.274?±?0.006‰ more positive than in vapors, respectively. The general \(\alpha - T\) equations describing the equilibrium Mg α in MgSiO₃ and Mg₂SiO₄ melt–vapor systems were: \(\alpha_{{{\text{Mg}}\left( {\text{l}} \right) - {\text{Mg}}\left( {\text{g}} \right)}} = 1 + \frac{{5.264 \times 10^{5} }}{{T^{2} }}\left( {\frac{1}{m} - \frac{1}{{m^{\prime}}}} \right)\) and \(\alpha_{{{\text{Mg}}\left( {\text{l}} \right) - {\text{Mg}}\left( {\text{g}} \right)}} = 1 + \frac{{5.340 \times 10^{5} }}{{T^{2} }}\left( {\frac{1}{m} - \frac{1}{{m^{\prime}}}} \right)\), respectively, where m is the mass of light isotope ²⁴Mg and m′ is the mass of the heavier isotope, ²⁵Mg or ²⁶Mg. These results offer a necessary parameter for mechanistic understanding of Mg isotope fractionation during evaporation and condensation that commonly occurs during the early stages of planetary formation and evolution.     

天津滨海新区抽水引起地面沉降现场试验研究

在天津滨海新区中新生态城服务中心开展了承压含水层抽水引起地面沉降的现场试验,采用振弦式孔隙水压力传感器和一孔多标数据采集器实时监测孔隙水压力和分层标的变化,分析了土体的变形性质和分层沉降的规律。试验结果表明,黏土层变形明显滞后于承压含水层水位的变化,以塑性变形为主且存在蠕变现象,而砂层既存在弹性变形,也存在一定的塑性变形和蠕变性。短期抽取地下水会使抽水井附近承压含水层上覆土体出现上小下大的沉降规律,且最大沉降出现在有明显孔隙水压力变化的土层顶板位置。