海带雌性原生质体的分离与培养

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。     

Integrative taxonomic description of Plakina kanaky,a new polychromatic sponge species from New Caledonia (Porifera: Homoscleromorpha)

Four similar sponges of different colors, all unknown to science, were collected in submarine caves of New Caledonia. We aimed at determining whether the four chromotypes represented different species or phenotypic variations of a unique new species. We used an integrative taxonomic approach combining morphologic, molecular and metabolomic analyses. The main traits that define these specimens are a skeleton made of monolophose, trilophose and tetralophose calthrops only, high chemical diversity and a high abundance and diversity of prokaryotic symbionts. The symbiotic community includes two unique prokaryote morphotypes, which are described for the first time in Homoscleromorpha, and appeared to be vertically transmitted. Although several features slightly differ among chromotypes, the most parsimonious conclusion was to propose a single new species Plakina kanaky sp. nov. Our phylogenetic analysis indicated the paraphyly of the Plakina genus, with P. kanaky sp. nov. belonging to a clade that includes Plakina jani and Plakina trilopha. The present work demonstrates that integrative taxonomy should be used in order to revise the entire Plakinidae family and especially the non‐monophyletic genus Plakina.     

Geochemistry and potential use of groundwater in the Rocca Busambra area (Sicily, Italy)

In the Rocca Busambra area (mid-west Sicily, Italy), from November 1999 to July 2002, 23 water points including wells and springs were sampled and studied for their chemical and isotopic compositions. Two rain gauges were also installed at different altitudes, and rainwater was collected monthly to determine the isotopic composition. The obtained results revealed the Rocca Busambra carbonate complex as being the main recharge area on account of its high permeability value. From a chemical view point, two main groups of water can be distinguished: calcium–magnesium–bicarbonate-type and calcium–magnesium–chloride–sulphate-type waters. The first group reflects the dissolution of the carbonate rocks; the second group probably originates from circulation within flyschoid sediments. Three water wells differ from the other samples due to their relatively high Na and K content, which probably is to be referred to a marked interaction with the “Calcareniti di Corleone” formation, which is rich in glauconite [(K, Na)(Fe³⁺, Al, Mg)₂(Si, Al)₄O₁₀(OH)₂]. In accordance with WHO guidelines for drinking water (2004), almost all the samples collected can be considered drinkable, with the exception of four of them, whose NO₃ ⁻, F⁻ and Na⁺ contents exceed the limits. On the contrary, the sampled groundwater studied is basically suitable for irrigation.     

Refinement of the late Quaternary geologic history of Erebus volcano,Antarctica using Ar/Ar and Cl age determinations

Six new ⁴⁰Ar/³⁹Ar and three cosmogenic ³⁶Cl age determinations provide new insight into the late Quaternary eruptive history of Erebus volcano. Anorthoclase from 3 lava flows on the caldera rim have ⁴⁰Ar/³⁹Ar ages of 23 ± 12, 81 ± 3 and 172 ± 10 ka (all uncertainties 2σ). The ages confirm the presence of a second, younger, superimposed caldera near the southwestern margin of the summit plateau and show that eruptive activity has occurred in the summit region for 77 ± 13 ka longer than previously thought. Trachyte from “Ice Station” on the eastern flank is 159 ± 2 ka, similar in age to those at Bomb Peak and Aurora Cliffs. The widespread occurrences of trachyte on the eastern flank of Erebus suggest a major previously unrecognized episode of trachytic volcanism. The trachyte lavas are chemically and isotopically distinct from alkaline lavas erupted contemporaneously in the summit region < 5 km away.     

Constraints on spatial variability in soft-sediment communities affected by contamination from an Antarctic waste disposal site

A small-scale (<500 m length) transect-based survey was conducted in December 1998 to examine the spatial distribution of soft-sediment communities and of concentrations of heavy metals and hydrocarbons in sediments in Brown Bay, adjacent to an abandoned waste dump, at Casey Station, Antarctica. Samples were taken along three transects at increasing distances (nine stations) from the shore-line waste dump. A gradient of contamination was detected, but concentrations of contaminants were very variable with "hotspots" or high levels of contaminants at some stations. Multivariate analysis revealed that the distribution of soft-sediment communities was distinctly different between the inner, middle and outer stations. Abundances of most taxa were very variable with few patterns apparent, but some fauna displayed an abundance gradient from the inner to the outer part of the bay. Many taxa had maximum abundances at outer stations and minimum at inner stations. Multivariate correlations between environmental variables and soft-sediment communities indicated that combinations of some metals (Cd, Cu, Sn, Pb) and grain size (mainly finer fractions, fine sands and coarse silts) were the variables that best "matched" the community patterns within Brown Bay. This study indicated that there were significant correlations between the presence of contaminants and the distribution and composition of soft-sediment communities over very small spatial scales.     

Solar flare injection and propagation of low-energy protons and electrons in the event of 7–9 July, 1966

Simultaneous observations of the 7–9 July 1966 solar particle event by energetic particle detectors on three satellites, IMP-III, OGO-III and Explorer 33 are utilized to show that large spatial gradients are present in the fluxes of 0.5–20 meV protons and 45 keV electrons. The event is divided into three parts: the ordinary diffusive component, the halo, and the core. The core corotates with the interplanetary field, and therefore it and the surrounding halo are interpreted as spatial features which are connected by the interplanetary magnetic field lines to the vicinity of the flare region. Upper limits to the interplanetary transverse diffusion coefficient for 4–20 meV protons at 1 AU are derived from the width of the halo. These are at least two orders of magnitude less than the parallel diffusion coefficient for the same energy particles.It is argued that the observed flux variations cannot be explained by an impulsive point source injection for any physically reasonable diffusion model. Instead, since the interplanetary transverse-diffusion coefficient is small for these low-energy particles, the observed spatial features are interpreted as the projection to 1 AU by the interplanetary field lines of an extensive injection profile at the sun. The geometry of the injection mechanism is discussed and it is suggested that some temporary storage of the flare particles occurs near the sun.Now at NASA, Goddard Space Flight Center, Greenbelt, Md., U.S.A.     

Baryon loading and the Weibel instability in gamma-ray bursts

The dynamics of two counter-streaming electron–positron–ion unmagnetized plasma shells with zero net charge is analysed in the context of magnetic field generation in gamma-ray burst internal shocks due to the Weibel instability. The effects of large thermal motion of plasma particles, arbitrary mixture of plasma species and space charge effects are taken into account. We show that, although thermal effects slow down the instability, baryon loading leads to a non-negligible growth rate even for large temperatures and different shell velocities, thus guaranteeing the robustness and the occurrence of the Weibel instability for a wide range of scenarios.     

Mesoproterozoic Fraser Complex: Geochemical evidence for multiple subduction‐related sources of lower crustal rocks in the Albany‐Fraser Orogen,Western Australia

The 1300 Ma Fraser Complex in the Albany‐Fraser Orogen of Western Australia is a thrust stack of mainly gabbroic rocks metamorphosed to granulite facies. This package of fault‐bounded units was elevated from a deep crustal level onto the margin of the Yilgarn Craton during continental collision between the Mawson and Yilgarn Cratons. Incompatible trace‐element distributions demand at least three mantle sources. Primitive‐mantle‐normalised incompatible‐element distributions show strong negative Ta–Nb anomalies, typical of subduction‐derived magmas. Three lines of evidence indicate that the mafic magmas did not acquire these anomalies by assimilation of crustal rocks: (i) major‐element compositions do not allow appreciable contamination with felsic material; (ii) Ni contents of many mafic rocks are too high for a significant contribution from a felsic assimilant; and (iii) Sr and Nd isotopic data support a largely juvenile source for the magmas that produced the Fraser Complex. Hence, the Ta–Nb anomalies are interpreted to reflect subduction‐related magmatic sources. On multielement diagrams, depletions in Sr, Eu, P, and Ti can be explained by fractional crystallisation, whereas Th and Rb depletions in many of the Fraser Complex rocks probably reflect losses during granulite‐facies metamorphism. These results suggest that the lower crust in this region at 1300 Ma was dominantly of arc origin, and there is no evidence to support mantle plume components. The Fraser Complex is interpreted as remnants of oceanic arcs that were swept together and tectonically interleaved with the margin of the Mawson Craton just before, or during, collision with the Yilgarn Craton at 1300 Ma.     

Determination of the Jenkinson and Collison’s weather types for the western Mediterranean basin over the 1948-2009 period. Temporal analysis

We determined the weather type, according to the Jenkinson and Collison procedure, of the 22 646 days in the 1948-2009 period for the western Mediterranean basin. The analysis is based upon the surface pressure values of the NCEP/NCAR reanalysis, for a grid of nine points with extreme vertices at 45º N, 5º W and 35º N, 15º E, which provides a broad synoptic catalogue for this region. We analyzed the trends of the types and their different groupings during the same period. The most frequent type is U (undetermined), with an annual average of approximately 100 days (99.4, 27.2%), followed by type A (anticyclone), with 75.5 days/ year (20.7%), and C (depression), with 67.8 days/year (18.6%). The high frequency of type U is due to the habitual pressure of baric fields with a low gradient over Mediterranean waters in the warm half of the year. According to their directions, the types from the west are the most frequent and those from the south, the least. The monthly regime of the most frequent types and groupings is quite regular; type C groups, as well as advective and cyclonic curvature groups, present summertime minima and maxima in the cold half of the year, whereas the opposite occurs with types U and A. The main statistically significant annual trends in the 1948-2009 period involve a decrease in type A (–4.19 days/decade, that is, –29.0%) and an increase in type U, the cyclonic types and those presenting an easterly component. On comparing the 31-yr sub-periods 1948-1978 and 1979-2009, the tendencies of A and U were confirmed, and increases can generally be seen in the types presenting an easterly component and a decrease in those with a westerly component. The variation in type A ranged from 2490 days in the first sub-period to 2192 in the second one (p = 0.000), mainly concentrated in summer and autumn. This evident reduction of type A coincides, paradoxically, with an increase in the sea surface pressure variable (+0.31 hPa/decade) throughout the 62 years of analysis. The negative trend found in type A differs from the results of some studies. The different analysis periods, the different scales or areas of study and the variety of methods used to determine the weather types can account for the fact that these results are discordant. Moreover, warming over the last few decades in the waters of the western Mediterranean basin, as well as the clearly cyclogenetic character of the gulfs of Lion and Genoa, might account for the decrease in type A and the increase in the cyclonic curvature types.     

Light curve analysis of the short period eclipsing binary RT and

We have analysed the 1978 UBV photoelectric observations of the short-period (RS CVn type) binary system RT And, using the rectifiable methods of Russell and Merrill (1952) and Kitamura (1965). The primary minimum is a transit. The solutions allow us to classify the system as a detached one, but a certain disagreement results with respect to solutions of previous epochs. A graphical test of the reliability of the solution is given.