Macrofauna impact on Ulva rigida C. Ag. production and relationship with environmental variables in the lagoon of Venice

The grazing pressure of the macrofaunal invertebrates associated with the biomass of Ulva rigida in the Venice lagoon, their species composition and relationship with environmental variables have been studied. Fifteen sampling campaigns were carried out during different seasons both in the central basin of the lagoon, dominated by macroalgae (especially U. rigida C. Ag.) and in the southern basin, prevalently populated by seagrasses (especially Zostera marina L.). Replicate experiments were conducted in the field by exposing Ulva fronds in net cages of 10 and 1 mm (control) mesh-sizes to allow or prevent grazer entrance. The grazing pressure was determined as Ulva growth rate difference in the cages. In the absence of invertebrate herbivores, Ulva exhibited per cent relative growth rates (%RGRs) ranging from 1.5 to 9.5% day(-1), whereas in their presence the %RGRs were significantly lower (from -2.5 to 3.4% day(-1)) and frequently negative, especially in the station dominated by macroalgae. In this area, peak grazing rates and macrofauna biomasses of up to 8.6% day(-1) and 1,480 g m(-2) fwt (84.4 ash-free dry weight), respectively, were found. On the whole, during in field experiments in the Ulva-dominated station, herbivores removed an amount of biomass whose percentage ranged from 59 to 165% (mean: 103%) of the biomass yield (grazers excluded) found in the cages. These results suggest the possibility that grazers could act as an important factor affecting Ulva production in the Venice lagoon. Macrofauna populations were analysed by means of multivaliate techniques applied to biological variables only and biological and environmental variables together. Data of individual abundance, after a log(x + 1) transformation and the calculation of the Bray-Curtis matrix, were classified using the Cluster Analysis and ordinated by means of the Non-Metric Multidimensional Scaling (MDS) technique, in accordance with the strategies used in the study of multispecies distributions. Finally, biological and environmental variables were analysed together by means of correlation matrices and the Principal Component Analysis.     

Oxygen Uptake During Repeated Exposure to Temperature Change: Physiological Divergence in Panamanian Cognate Pairs and Latitudinally Distant Populations of Decapod Crustacea

Abstract. The emergence of the Isthmus of Panama subdivided the amphi-American biota. In the present study, Pacific and Atlantic populations of four cognate pairs of crabs were used to discern whether exposure to different thermal regimes in habitats, in the putative absence of gene flow, has resulted in physiological divergence. Populations that potentially form a common genetic pool were also used; these were populations of the Atlantic Panama cognate that occur in Belize and Florida. Decreases in water temperature occur periodically in Pacific Panama and Florida, but not in Atlantic Panama or Belize. In this study, physiological divergence in oxygen uptake was assessed in response to repeated exposure to either control and decreased temperature or control and increased temperature. Results indicate that, in only some of the genera tested, exposure to decreases in habitat temperature has resulted in divergence. Partial support is found for the corollary that adaptation to an environment with periods of decreased temperature results in reduced compensation during exposure to elevated temperature.     

The biokinetic behavior of Tc in juvenile Coho Salmon, Oncorhynchus kisutch (Walbaum)

Juvenile coho salmon, Onchorynchus kisutch, were exposed to ^95mTc in both saltwater and freshwater to study the biokinetic behavior of this element in an anadromous fish. In freshwater, equilibrium whole-body concentration factors (CF₅) ranged from 28 to 32 in two separate experiments, in sharp contrast to an equilibriumCF of < 5 in saltwater. While the uptake kinetics were unimodal in saltwater, in freshwater, biomodal uptake kinetics were observed.Technetium-95m retention was biphasic in freshwater depuration experiments, an initial rapid loss of 21% to 26% of the steady-state activity with an elimination half-life of 3 to 5 days was followed by a slower loss rate, with a whole-body elimination half-life ranging from 23 to 33 days. In saltwater, retention was monophasic with an elimination half-life of 5–16 days. Dramatic decreases in ^95mTc body burdens when freshwater-labeled fish are transferred to Tc-labeled saltwater indicate that the changes in physiology accompanying saltwater acclimation lead to the loss of ^95mTc.     

Variability in concentration of suspensates in an estuarine environment

Three data sets of suspensate concentrations, collected under different sampling plans, were analyzed to develop a minimal sampling period, consistent with a predetermined precision. Individual samples collected during any part of a given day were within ±50% of the daily average. In the main channel of the estuary, a monthly sampling scheme closely approximated a weekly pattern; however, seasonal sampling was unsatisfactory. Outside the main channel, weekly, monthly, and seasonal sampling patterns did not differ greatly. Variability in suspensate concentrations must be considered when calculating sediment flux in estuarine and coastal waters.     

Spectral-temporal structure of variations in the atmospheric total ozone in central Eurasia

The results of long-term (1980–2003) systematic measurements of the total ozone content at the Issyk Kul station (42.6° N, 77.0° E; 1650 m above sea level) are presented. The statistical characteristics and spectral structure of variations in the total ozone and the main tendencies of its temporal variability are determined. It is found that the total ozone content decreased in 1980–2003 at an average rate of (?1.29±0.08) DU/yr. The results of Fourier and wavelet analyses have shown that only oscillations with periods of 12, 27–29, and 102–105 months are rather stable and can be represented as harmonic oscillations. Oscillations with periods shorter than six months have the character of periodically arising pulsations. Among these, oscillations with periods of 27–29 and 34–37 days can be distinguished. It is noted that the spectral-temporal structure of variations in the total ozone content obtained from ground-based measurements at the Issyk Kul station is in good agreement with the corresponding structure obtained from TOMS satellite measurements.     

Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.     

A detailed survey near Mount Eratosthenes Eastern Mediterranean

A detailed marine survey made to the South West of Mount Eratosthenes is described. The results show the existence of a narrow zone of WNW trending deformed sediment west of the mount surrounded by an evaporite sequence in different stages of deformation.This work was intended to settle the conflict between the results of three previous interpretations of the tectonic structure of this area. We see the area being characterised by five areas with different degrees of tectonic deformation yet showing little causal relationship to overburden of Nile Cone sediments. A fault controlled cliff coincides with the previously described salt wall and strike slip fault yet we see no evidence for its diapiric origin nor positive evidence for strike slip motion along it. We concur with the previously observed area of higher free-air gravity anomalies termed the Eratosthenes tectonic zone, although it is traversed by several boundaries between areas of different deformation. Our results thus show some agreements and some re-interpretations of the observations. This complicated region is not yet fully understood however.     

Algorithm for non-hydrostatic dynamics in the Regional Oceanic Modeling System

A non-hydrostatic algorithm for the Regional Oceanic Modeling System (ROMS) is proposed. It is based on a decomposition technique for hydrostatic and non-hydrostatic pressure. The algorithm has a pressure-correction scheme with split-explicit time-stepping for baroclinic and barotropic vertical modes with a free surface. The algorithm implementation requires solving a Poisson equation for a non-hydrostatic pressure that has a non-symmetric matrix in discrete form. The efficiency of a different class of solvers and preconditioners were tested. The algorithm is successfully implemented with several examples where non-hydrostatic effects are important. These include standing external gravity waves; strongly nonlinear internal wave generation and transformation; stratified shear instability and its associated mixing; and nonlinear internal tidal generation over a ridge. The corresponding changes in the pre-processing and post-processing infrastructure in the existing hydrostatic ROMS code were performed to implement parallel elliptic solvers and a new set of dynamical equations.