海带雌性原生质体的分离与培养

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。     

Sea-level and subsidence data from a Late Holocene back-barrier lagoon (Valle Standiana, Ravenna, Italy)

Late Holocene back-barrier sediments have been studied in a former lagoon reclaimed during the last century. Several shallow boreholes were cored, from which nine organic-rich samples were selected for radiocarbon dating, and others for palynological and palaeontological analysis. This enabled the reconstruction of the evolution of a back-barrier coastal basin during the late Holocene coastline progradation. Since late Roman times, the rate of relative sea-level rise (RSLR) has increased from 1.1 mm/yr to over 2 mm/yr, even after removal of the main human impacts during of the last century. This change is ascribed to sediment compaction related to changes in the hydrological regime, and possibly to an isostatic subsidence wave accompanying the northward shift of the Po Delta during the last 2000 years.     

An early Miocene reefal platform in the Rama Ridge (Banda Sea, Indonesia)

 In December 1995 we dredged early Miocene coral-reef carbonates and early/middle Pliocene slope carbonates outcropping on the submerged Rama Ridge. This indicates that some of the Banda Sea Ridges were present during early Miocene times. Subsequent major tectonic subsidence occurred between middle Miocene and early Pliocene times. These results concur with the hypothesis of a relatively recent age for the North Banda Sea basement. Received: 5 March 1997 / Revision received: 23 October 1997     

Experimental investigation of response stability and transition behaviour of a nonlinear ocean structural system

This study experimentally investigates the nonlinear response stability and transition behaviour of a submerged, moored ocean structural system which consists of a spherical buoy and attached multi-point mooring lines. The system is excited by a periodic wave field in a closed channel. System nonlinearities include complex geometric restoring (stiffness) force and coupled fluid-system interaction exciting forces. Experimental set-up, operating procedures and analysis of the measured results are presented. Characteristic motions observed include harmonic, subharmonic and ultraharmonic responses, which demonstrate a signature of the intricate pattern of the nonlinear global behaviour. Good agreements between the measured and most predicted responses are demonstrated in both time and frequency domains. These results confirm the validity of the analytical model presented and calibrate the accuracy of the existing numerical predictions. Primary and secondary resonances in the response are identified via frequency response curves. Response bifurcation cascades are observed in the experimental results and the possible existence of higher-order nonlinear responses is inferred.     

Modelling the long-term distribution of significant wave height with the Beta and Gamma models

The paper suggests modelling the long-term distribution of significant wave height with the Gamma, Beta of the first and second kind models. The three models are interrelated, flexible and cover the three different tail types of Extreme Value Theory. They can be used simultaneously as a means of assessing the uncertainty effects that result from choosing equally plausible models with different tail types. This procedure is intended for those applications that require the long-term distribution of significant wave height as input rather than the prediction of extreme values. The models are fitted to some significant wave data as an illustration. Details about maximum likelihood estimation are given in A.     

Impact of grazing by benthic eukaryotic organisms on the nitrogen sediment–water exchange in the North Sea

The metabolic inhibitor cycloheximide was used to estimate the influence of primarily unicellular eukaryotes (heterotrophic protozoa) on nutrient recycling in different types of sediments in the North Sea. Fluxes of dissolved inorganic nitrogen across the sediment–water interface were measured in undisturbed sediment cores (controls) and compared to fluxes in sediment cores with cycloheximide added. If eukaryotes play an important role in nutrient recycling, one would expect to find lower nitrogen sediment–water effluxes in cores with cycloheximide due to the inactivation of eukaryotes. This important role hypothesised for eukaryotes was not generally observed: Only at four of the nineteen stations were ammonium effluxes significantly higher in controls than in cores with cycloheximide, and at five stations nitrate effluxes were significantly higher in the controls than in the cores with cycloheximide. Eukaryotic activity apparently contributed to the sediment–water exchange of ammonium through mineralisation of organic matter, nitrification and the subsequent release of ammonium and nitrate at these stations. At most other stations no differences were obtained between controls and cores with cycloheximide. This suggests that bacteria were the most important nutrient mineralisers at these stations at the time of the cruises.     

Submarine groundwater discharge and benthic biogeochemical zonation in the Huanghe River Estuary

Submarine groundwater discharge(SGD) has received increasing attention by studies on coastal areas; however,its effects on biogeochemical zonation have not been investigated to date. The Huanghe River Estuary(HRE) is a world class river estuary with high turbidity, and heavy human regulation. This study investigated how SGD is related to the benthic biogeochemistry of the HRE. Based on the distribution of several parameters(e.g., salinity,temperature, dissolved oxygen(DO) levels, p H, radium iso...     

Long-term moored array measurements of currents and hydrography over Georges Bank: 1994–1999

In conjunction with the GLOBEC (Global Ocean Ecosystems Dynamics) program, measurements of moored currents, temperature and salinity were made during 1994–1999 at locations in 76 m of water along the southern flank of Georges Bank and at the Northeastern Peak. The measurements concentrate on the biologically crucial winter and spring periods, and coverage during the fall is usually poorer.Current time series were completely dominated by the semidiurnal M₂ tidal component, while other tidal species (including the diurnal K₁ component) were also important. There was a substantial wind-driven component of the flow, which was linked, especially during the summer, to regional–scale response patterns. The current response at the Northeast Peak was especially strong in the 3–4 days period band, and this response is shown to be related to an amplifying topographic wave propagating eastward along the northern flank. Monthly mean flows on the southern flank are southwestward throughout the year, but strongest in the summertime. The observed tendency for summertime maximum along-bank flow to occur at depth is rationalized in terms of density gradients associated with a near-surface freshwater tongue wrapping around the Bank.Temperature and salinity time series demonstrate the presence, altogether about 25% of the time, of a number of intruding water masses. These intrusions could last anywhere from a couple days up to about a month. The sources of these intrusions can be broadly classified as the Scotian Shelf (especially during the winter), the Western Gulf of Maine (especially during the summer), and the deeper ocean south of Georges Bank (throughout the year). On longer time scales, the temperature variability is dominated by seasonal temperature changes. During the spring and summer, these changes are balanced by local heating or cooling, but wintertime cooling involves advective lateral transports as well. Salinity variations have weak, if any, seasonal variability, but are dominated by interannual changes that are related to regional- or basin-scale changes.All considered, Georges Bank temperature and salinity characteristics are found to be highly dependent on the surrounding waters, but many questions remain, especially in terms of whether intrusive events leave a sustained impact on Bank waters.     

Describing and generating facies models for reservoir characterisation: 2D map view

This paper documents a new method for describing channel-related sedimentary deposits based on formal language theory. Using this method an analogue model of a sedimentary deposit can be encoded as a grammar. A program, called a parser, has been developed which can generate stochastic maps of these sedimentary deposits based on information in a specified grammar. The maps of sedimentary deposits generated by the parser have the same type, spatial arrangement, shape and size distribution as the analogue model. The successful generation of depositional maps represents a crucial step in the ongoing development of a new technique designed to generate 3D static geological models of sedimentary successions. The maps can be conditioned to match sparse hard data in the form of channel segments interpreted from seismic horizon maps.