Acute and chronic toxicity of the rice herbicides thiobencarb and molinate to opossum shrimp (Neomysis mercedis)

Neomysis mercedis were exposed to the rice herbicides molinate and thiobencarb, which have been detected in the Sacramento-San Joaquin Delta. Continuous-flow acute and chronic toxicity studies were performed. For thiobencarb, the 4-, 7- and 14-day LC_sos were 304, 214 and 91 μg/liter, respectively. For molinate, these values were 9910, 2530, and 820 μg/liter, respectively. Mortality stabilized after 18 and 28 days exposure, respectively, for thiobencarb and molinate. The incipient lethal concentrations were 53 and 230 μg/liter, respectively. Joint toxicity studies indicated additive toxicity for the two chemicals. Chronic no observable effects concentrations (NOECs) were estimated at 3·2 and 25·6 μg/liter for thiobencarb and molinate, with measurable effects occurring at 6·2 and 45·2 μg/liter, respectively. Environmental monitoring data suggests that concentrations of these chemicals in the Delta prior to 1985 may have exceeded the threshold for chronic toxicity, under conditions of joint exposure. However, due to continued improvements in pesticide management practices, environmental concentrations decreased to approximately 30 percent of the chronic threshold for joint toxicity by 1988. Baseline data on growth and reproduction relevant to toxicity tests are also presented for this species.     

Behavioural Effects of Chemically Dispersed Oil and Subsequent Recovery in Diploria strigosa (Dana)

Abstract. Survival and behaviour of the hermatypic coral Diploria strigosa was studied during 6–24 h doses with water-accomodated fractions of chemically dispersed crude oil, and for a subsequent recovery period of 1 month. Experiments utilized a flow-through laboratory dosing procedure and incorporated petroleum hydrocarbon measurements in order to simulate a major but short-term oil spill in shallow subtidal benthic reef environments. Chemically dispersed oil treatments consisted of Arabian Light Crude oil with Corexit 9527 or BP1100WD at 1–20 ppm concentrations of oil.
In general, effects observed were sub-lethal, temporary, and associated with the highest concentrations tested. Responses to the presence of dispersed oil at 20ppm for 24 h included mesenterial filament extrusion, extreme tissue contraction, tentacle retraction and localized tissue rupture. The nature and severity of reactions during the dosing phase varied between colonies and treatments, but colonies typically resumed normal behaviour within 2 h to 4 d of the recovery period. It therefore seems unlikely that observed biological effects would impair long-term viability.     

Long term prediction of non-linear vertical bending moments on a fast monohull

An assessment is made of the maximum wave induced bending moment expected to occur during the operational lifetime of a fast monohull, based on long term distribution calculations of the non-linear vertical bending moment at mid-ship. The ship is assumed to operate in the Northern North Sea, with an operational life of 25 years. A succession of short-term stationary ship responses represented by Rayleigh distributions of peaks results in the long-term distribution of the structural loads. Non-linear pseudo transfer functions are used in the procedure to calculate the variances of the short-term responses. The amplitudes of these transfer functions are calculated by a non-linear time domain seakeeping program. The results are compared with rules minimum required values and also with results from simpler calculation procedures such as adopting design sea states.     

Composition and Useful Properties of Tailings of Marine Manganese Nodules and Crusts

Marine manganese nodules and crusts, when processed, yield tailings which may be utilized for environmental and economic benefit. The key to the reasonable and effective utilization of these tailings lies in making a systematic appraisal of their composition and properties. This article gives an introduction to the investigation of manganese tailings properties. The tailings have a high iron and/or manganese content, high surface area, high porosity, and fine grain size. Some tailings have a high rare earth element content which is valuable. They may also have high SO₃, arsenic, and uranium contents which are harmful. Depending on the process used to produce the tailings, there will likely be some differences in chemical composition, mineral assemblages, surface area and adsorption capability, pore diameter and volume, density and pH. In assigning potentially beneficial applications for these tailings, these differences should be taken into account to optimize utilization.     

The buckling of plastic oblate hemi-ellipsoidal dome shells under external hydrostatic pressure

The paper presents a theoretical and an experimental investigation into the buckling of seven oblate hemi-ellipsoidal dome shells under external hydrostatic pressure. Four of the shells were made in glass reinforced plastic and three were made from a thermosetting plastic called solid urethane plastic. All the vessels were tested to destruction. The theoretical study was made with the aid of a non-linear finite element solution, where both geometrical and material non-linearity were allowed for. Good agreement was found between experiment and theory for all the vessels. The very oblate domes failed axisymmetrically. Theoretical convergence was good for the more oblate domes but it was not as good as for the less oblate domes. This may have been because the less oblate domes did not fail in a classical axisymmetric manner as was expected. This work is of much importance in ocean engineering.     

Seamount gravity anomaly modelling with variably thick sediment cover

Inversion modelling of marine gravity anomalies to derive predicted seafloor topography has provided significant advance in delineating deep-ocean bathymetry where the seafloor both conforms to the half-space cooling model of seafloor spreading, and largely sediment-free. Similar modelling for elevated ridges and seamounts, that are formed by processes other than seafloor spreading and/or have proximal sediment sources (e.g., continental margins and volcanic arcs), have significantly higher errors when validated against modern shipborne echo-sounding data. A three-dimensional, five-layer gravity model is emulated for the cases of both synthetic and real seamounts, with varying degrees of sediment burial, to establish the sensitivity of variable sediment cover as a source of error. A simple `Gaussian' seamount with base radius of 30 km, 2000 m of relief, has a maximum 140–160 mGal anomaly, that decreases to 50 mGal with the addition of 1 km of sediment cover with simple `flood' geometry. Complete burial, with a typical sediment density of 2300 kg m^–3, results in a 120 mGal difference from a sediment-free seamount model. Increasing sediment density results in an exponential decay of the seamount anomaly. More complex synthetic geometries of varying basement relief and sediment thickness show that the anomaly amplitude remains significant, especially where the latter is >700–800 m thick. For the real case, seamounts of the Three Kings Ridge (northern New Zealand) imaged with seismic reflection data, with varying degrees of sediment cover of up to 1 km, when modelled both with and with-out the inclusion of a sediment layer, typically have rms differences of 30 mGal between observed and modelled gravity anomalies. Significantly, the rms errors are reduced by 50% with the inclusion of a sediment layer that corresponds to a reduction of predicted seafloor topography rms errors of 192–684 m to 78–360 m.     

The Spatial Distribution of Caprellidea (Crustacea: Amphipoda): A Stress Bioindicator in Ceuta (North Africa, Gibraltar Area)

Abstract. The caprellid (Crustacea: Amphipoda) community associated with the alga Cystoseira usneoides (L.) Roberts 1967 was studied on a spatial scale in relation to the influence of environmental factors on the coast of Ceuta (North Africa) using multivariate analyses. Twenty-two stations were sampled and five environmental factors were tested (hydrodynamics, silting, suspended organic matter, organic matter in silt and suspended solids). The spatial distribution of the caprellid community reflected the physico-chemical conditions of the coast. Phtisica marina Slabber 1769 was the only species present at the stations of the harbour of Ceuta, characterised by the lowest values of hydrodynamics and the highest values of silting, suspended organic matter and suspended solids. The most exposed stations (high hydrodynamics and low values of silting, dissolved organic matter and suspension solids) were mainly dominated by Caprella danilevskii Czerniavskii 1868 and C. penantis Leach 1814. These two species have developed a "parallel" posture that enables them to attach strongly to the substratum and thus inhabit areas subjected to heavy exposure or strong currents. Caprella acanthifera Leach 1814 and Pseudoprotella phasma (Montagu 1804) preferred stations characterised by moderate values of silting and suspended solids, such as those located in the coastal areas in front of the city of Ceuta, but these species were not found at the most stressed harbour stations.     

A B-spline based higher order panel method for analysis of steady flow around marine propellers

A higher order panel method based on B-spline representation for both the geometry and the solution is developed for the analysis of steady flow around marine propellers. The self-influence functions due to the normal dipole and the source are desingularized through the quadratic transformation, and then shown to be evaluated using conventional numerical quadrature. By selecting a proper order for numerical quadrature, the accuracy of the present method can be increased to the machine limit. The far- and near-field influences are shown to be evaluated based on the same far-field approximation, but the near-field solution requires subdividing the panels into smaller subpanels continuously, which can be effectively implemented due to the B-spline representation of the geometry. A null pressure jump Kutta condition at the trailing edge is found to be effective in stabilizing the solution process and in predicting the correct solution. Numerical experiments indicate that the present method is robust and predicts the pressure distribution on the blade surface, including very close to the tip and trailing edge regions, with far fewer panels than existing low-order panel methods.     

Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.     

Late-Quaternary supply of terrigenous organic matter to the Congo deep-sea fan (ODP site 1075): implications for equatorial African paleoclimate

Late-Quaternary sections (about 1 Ma) from the Congo deep-sea fan (ODP Leg 175, site 1075) were used to reconstruct the terrigenous organic matter supply to the easternmost equatorial Atlantic Ocean. Variations in quantity and quality of the riverine organic matter reflect the interaction between the paleoclimatic development within the continental catchment area and the paleoceanographic conditions in the Congo river plume. To characterize the delivery of organic matter from terrigenous and marine sources, we used elemental and bulk carbon isotopic analyses, Rock-Eval pyrolysis, lignin chemistry, and organic petrology. High-amplitude fluctuations occurring about every 15-25 ka reveal a mainly precessional control on organic sedimentation. Results from Rock-Eval pyrolysis indicate a mixed kerogen type III/II, as would be anticipated in front of a major river. Fluctuations in T_max from Rock-Eval pyrolysis demonstrate pronounced cyclic changes in the delivery of low- and high-mature organic matter. Contribution of the low-mature organic fraction was strongest during warm climates supporting enhanced marine production offshore of the Congo. Organic petrological observations confirm the existence of abundant terrigenous plant tissues, both non-oxidized (vitrinite) and oxidized (inertinite). Charcoal-like organic matter (fusinite) is attributed to periods of increased bush fires in the continental hinterland, and implies more arid climatic conditions. Results from ratios of specific phenolic lignin components suggest that terrigenous organic matter in Late-Quaternary sections of site 1075 mainly derives from non-woody angiosperm tissue, i.e., grasses and leaves. Correlation between the amount of specific lignin phenols and the bulk '¹³C_org signature fosters the conclusion that an appreciable amount of the terrigenous organic fraction derives from C4 plant matter. This may cause an underestimation of the terrigenous proportion of bulk organic matter when assessments are based on bulk carbon isotopic signatures alone.