长江口及冲淡水区叶绿素a、细菌、ATP、POC及微生物呼吸作用速率之间的关系

1986年7月作者在长江口及其冲淡水区对水体细菌丰度、叶绿素a、ATP、POC的浓度和微生物呼吸作用耗氧速率进行了测定和研究。结果表明,高细菌丰度值出现在口门附近,与悬浮物浓度有关,在该区域,细菌是ATP的主要贡献者和溶解氧的主要消耗者,ATP与POC之间存在着相关性。在稀释区(盐度25—30),由于硅藻的旺发使浮游植物取代了细菌而成为ATP的主要贡献者,同时浮游植物的呼吸作用成为溶解氧消耗的主要因素。叶绿素a和ATP之间以及ATP和POC之间均存在着明显的相关性。文章还估测了微生物碳对POC的贡献。     

中型海洋围隔生态系中汞通量、形态和收支的研究

在海洋围隔生态系实验中加入迄今最低浓度的无机汞,以研究汞的迁移通量、形态分布和收支。加汞后,汞从水柱中被排除的行为可用一级反应动力学描述。在该生态系内汞被排除的半寿期,与初级生产力和浮游植物特性如个体大小、浮游能力等有关。对照围隔生态系内,0.01μg Hg/cm~2·a的汞垂直通量与海湾现场测量值一致。在自然条件下,溶解汞是水中的主要形态,占73％。加汞后,颗粒汞迅速上升,可达70％以上。浮游植物对汞的富集因子为10~5级。汞在沉积物、器壁和水体中的回收只占52.3％。     

Implications of seasonal priming and reproductive activity on the interpretation of Comet assay data derived from the clam, Tapes semidecussatus Reeves 1864, exposed to contaminated sediments

We explore the use of the clam Tapes semidecussatus Reeves 1864 as an indicator for the presence of potentially genotoxic substances in estuarine sediments. The limitations associated with the interpretation of Comet assay data (expressed as % DNA in tail) in terms of clam reproductive state, size (age) and thermal exposure history following laboratory acclimation are discussed. Hatchery-reared clams, subjected to ambient temperature fluctuations during growth, were exposed in vivo under laboratory conditions for three weeks to sediment samples collected from a polluted site and a "clean" reference site. The DNA damage observed in haemocytes, gill and digestive gland cells was significantly higher in animals exposed to contaminated sediment compared to those exposed to sediment from the reference site. The extent of DNA damage recorded was not correlated with size (age). Spawning was not observed during the experiment. Nevertheless, clams with well-developed gonads showed a statistically higher degree of DNA damage in gill and digestive gland cells- but not haemocytes, demonstrating an increased sensitivity to potential genotoxic compounds, possibly caused by impaired DNA repair capacity due to reproductive activity. Furthermore, the degree of DNA damage in clams exposed to contaminated sediments was higher in autumn and winter compared to spring and summer, suggesting an effect of seasonal priming.     

Esterification of vertebrate-like steroids in the eastern oyster (Crassostrea virginica)

The esterification of two model vertebrate steroid hormones - estradiol (E2) and dehidroepiandrosterone (DHEA) - was studied in the oyster Crassostrea virginica. The activity of acyl-CoA:steroid acyltransferase was characterized in microsomal fractions isolated from oyster digestive glands. The apparent Km and Vmax values changed with the fatty acid acyl-CoA used (C20:4, C18:2, C18:1, C16:1, C18:0 or C16:0), and were in the range of 9-17 microM, and 35-74 pmol/min/mg protein for E2, and in the range of 45-120 microM, and 30-182 pmol/min/mg protein for DHEA. Kinetic parameters were also assessed in gonadal tissue. The enzyme saturated at similar concentrations, although conjugation rates were lower than in digestive gland. Preliminary data shows that tributyltin (TBT) in the low microM range (1-50) strongly inhibits E2 and DHEA esterification, the esterification of E2 being more sensitive to inhibition than that of DHEA. Overall, results indicate that apolar conjugation occurs in oysters, in both digestive gland and gonads, at a very similar rate to mammals, suggesting that this is a well conserved conjugation pathway during evolution. Esterification, together with other mechanisms, can modulate endogenous steroid levels in C. virginica, and might be a target for endocrine disrupters, such as TBT.     

A hybrid model for numerical wave forecasting and its implementation——Ⅰ. The wind wave model

The authors make an endeavor to explain why a new hybrid wave model is here proposed when several such models have already been in operation and the so- called third generation wave modej is proving attractive. This part of the paper is devoted to the wind wave model. Both deep and shallow water models have been developed, the former being actually a special case of the latter when water depth is great. The deep water model is exceptionally simple in form. Significant wave height is the only prognostic variable. In comparison with the usual methods to compute the energy input and dissipations empirically or by "tuning", the proposed model has the merit that the effects of all source terms are combined into one term which is computed through empirical growth relations for significant waves, these relations being, relatively speaking, easier and more reliable to obtain than those for the source terms in the spectral energy balance equation. The discrete part of the model and the implementation of the mode     

ON THE REGIONAL DIVISION OF THE MARINE ALGAL FLORA OF THE WESTERN NORTH PACIFIC

Although in the discussions of the geographic distribution of the marine algae in the western part of the North Pacific, the marine algal flora of this vast area has been variously divided by phycologist, there is, as yet, not a single satisfactory system for its regional division since in some cases the proposed floristic regions are too large and inclusive resulting in their heterogenous nature and in other cases, they are too minute or classified according to their political nature. In the present paper, an attempt is made to propose a system of objectively dividing this vast area into a number of major fioristic regions, based on the following two principles;     

Seasonal variation of Zn, Pb, Cu and Cd concentrations in the root-sediment system of Spartina maritima and Halimione portulacoides from Tagus estuary salt marshes

Concentrations of Zn, Pb, Cu and Cd have been determined in leaves, stems and roots of Spartina maritima and Halimione portulacoides from the Tagus estuary salt mash (Corroios) and in the sediments between their roots. Biological materials and sediments were sampled every 2 months, between July 1991 and July 1992. Root biomass increased from July to September and from January to March. The greatest metal concentrations occurred in the roots, with lowest levels in January and increasing levels during the growth periods. Zn, Pb and Cu in sediments exhibited a corresponding change in concentrations, reaching maximum in January and subsequently decreasing in spring. The ratios between metal concentrations in the root and in sediments were higher for H. portulacoides when compared to S. maritima, whose roots are surrounded by a more acidic and reduced sediment environment. It was concluded, therefore, that H. portulacoides is a more effective accumulator of metals than S. maritima, and both root-sediment systems exhibited a seasonal variation of metal concentrations.     

Field application of lysosomal destabilisation indices in the mussel Mytilus galloprovincialis: biomonitoring and transplantation in the Lagoon of Venice (north-east Italy)

A field study was carried out in the Lagoon of Venice (north-east Italy) with the aim of evaluating the potential use of lysosomal destabilisation as a biomarker of anthropogenic stress in the autochthonous mussel Mytilus galloprovincialis. Two different approaches were adopted in biomonitoring six sites in the Lagoon, evaluating indigenous populations of mussels and organisms transplanted from a reference site and checked at several points in time. Lysosomal membrane stability was investigated by means of two tests: neutral red retention assay (NRRA) for evaluating haemocyte lysosomes and lysosomal latency test (LLT) for digestive cell lysosomes. Results indicate that the lysosomal response measured in haemocytes according to NRRA is a more valuable biomarker of anthropogenic stress in the framework both of passive and active biomonitoring in marine coastal environments.     

B[a]P-DNA binding in early life-stages of Atlantic tomcod: population differences and chromium modulation

Atlantic tomcod (Microgadus tomcod) from the Hudson River (HR) are resistant at the molecular and organismic levels to the effects of exposure to dioxin-like aromatic hydrocarbon (AH) compounds, but much less so to benzo[a]pyrene (B[a]P). The aims of this study were to determine in early life-stages of tomcod exposed to B[a]P: (1) if DNA binding levels differed between fish from the HR and Miramichi River (MR), and (2) if co-exposure to chromium could modulate this genotoxic effect. After exposure to [(3)H]B[a]P alone, DNA-bound radioactivity was 5-10-fold higher in embryos and larvae of MR than HR descent. Co-exposure to chromium modulated DNA binding levels in offspring of both populations. In MR embryos, co-exposure to chromium inhibited B[a]P uptake. These results demonstrated resistance to the genotoxic effects of B[a]P in early life stages of HR tomcod at an ecologically important endpoint and suggest the ability of chromium to modulate AH-induced genotoxicity.     

Expression and activity of a multixenobiotic resistance system in the Pacific oyster Crassostrea gigas

The multidrug resistance (MDR) mechanism corresponds to a defence system relying on the expression of high molecular membrane proteins that can actively lower the intracellular concentration of a wide variety of toxins, thus maintaining them below their toxic level. Using RT-PCR, expression levels of a gene belonging to the class I of mammalian mdr genes, has been assessed in different developmental stages of the oyster Crassostrea gigas. While no expression was found in the oocyte or the trocophore stage, a rise of mRNA content was observed from the veliger stage to the juvenile stage, thus indicating the induction of the system as the animal is developing in the environment. The incubation of gill fragments in the dye rhodamine B and subsequent measurements of intracellular fluorescence using a microplate reader indicates that the system can effectively decrease the accumulation of the test compound in a competitive manner with known inhibitors or environmental contaminants as observed in vertebrate cells. The oyster MXR system is thus becoming active in adult oyster and could be of importance in environmentally contaminated areas.