The Co-occurrence of Methanotrophic and Chemoautotrophic Sulfur-Oxidizing Bacterial Symbionts in a Deep-sea Mussel

Abstract. In April 1990 a new species of mytilid containing bacterial endosymbionts was discovered at a deep-water seep site within Alaminos Canyon in the Gulf of Mexico. Activities of ribulose bisphosphate carboxylase/oxygenase and ATP sulfurylase, as well as the presence of substantial levels of elemental sulfur in the gill tissues indicate the presence of chemoautotrophic sulfur-oxidizing symbionts in the gills. Methanol dehydrogenase activity and the tissue stable carbon isotope ratios indicate the presence of methanotrophic bacteria in the gills of the same animals. Two distinct size classes and morphological types of gram negative bacteria are visible in transmission electron micrographs of the gill tissue, one of which contains the complex internal membranes typical of methanotrophs. Both general types of symbionts have been demonstrated singly in related species of deep-sea mytilids. In this species, however, both types are found in single individuals, often within the same cell vacuole.     

Timing and pace of ice-sheet withdrawal across the marine–terrestrial transition west of Ireland during the last glaciation

Understanding the pace and drivers of marine-based ice-sheet retreat relies upon the integration of numerical ice-sheet models with observations from contemporary polar ice sheets and well-constrained palaeo-glaciological reconstructions. This paper provides a reconstruction of the retreat of the last British–Irish Ice Sheet (BIIS) from the Atlantic shelf west of Ireland during and following the Last Glacial Maximum (LGM). It uses marine-geophysical data and sediment cores dated by radiocarbon, combined with terrestrial cosmogenic nuclide and optically stimulated luminescence dating of onshore ice-marginal landforms, to reconstruct the timing and rate of ice-sheet retreat from the continental shelf and across the adjoining coastline of Ireland, thus including the switch from a marine- to a terrestrially-based ice-sheet margin. Seafloor bathymetric data in the form of moraines and grounding-zone wedges on the continental shelf record an extensive ice sheet west of Ireland during the LGM which advanced to the outer shelf. This interpretation is supported by the presence of dated subglacial tills and overridden glacimarine sediments from across the Porcupine Bank, a westwards extension of the Irish continental shelf. The ice sheet was grounded on the outer shelf at ~26.8 ka cal bp with initial retreat underway by 25.9 ka cal bp. Retreat was not a continuous process but was punctuated by marginal oscillations until ~24.3 ka cal bp. The ice sheet thereafter retreated to the mid-shelf where it formed a large grounding-zone complex at ~23.7 ka cal bp. This retreat occurred in a glacimarine environment. The Aran Islands on the inner continental shelf were ice-free by ~19.5 ka bp and the ice sheet had become largely terrestrially based by 17.3 ka bp. This suggests that the Aran Islands acted to stabilize and slow overall ice-sheet retreat once the BIIS margin had reached the inner shelf. Our results constrain the timing of initial retreat of the BIIS from the outer shelf west of Ireland to the period of minimum global eustatic sea level. Initial retreat was driven, at least in part, by glacio-isostatically induced, high relative sea level. Net rates of ice-sheet retreat across the shelf were slow (62–19 m a⁻¹) and reduced (8 m a⁻¹) as the ice sheet vacated the inner shelf and moved onshore. A picture therefore emerges of an extensive BIIS on the Atlantic shelf west of Ireland, in which early, oscillatory retreat was followed by slow episodic retreat which decelerated further as the ice margin became terrestrially based. More broadly, this demonstrates the importance of localized controls, in particular bed topography, on modulating the retreat of marine-based sectors of ice sheets.     

Evidence of 6 000-Year Periodicity in Reconstructed Sunspot Numbers

Reconstructed sunspot data are available that extend solar activity back to 11 360 years before the present. We have examined these data using Hurst analysis, a moving average filter, and Fourier analysis. All of the procedures indicate the presence of a long term (≈6 000 year) cycle not previously reported. A number of shorter cycles formerly identified in the literature by using Fourier analysis, Bayes methods, and maximum entropy methods were also detected in the reconstructed sunspot data.     

Raman microspectrometry of fluid inclusions

For many kinds of fluid inclusions, the coupling of microthermometry and Raman microspectrometry is still the only viable option to obtain compositions of single fluid inclusions. A review is given on the basis of 16 years of experience and helped with about 120 references of the instrumentation, analytical conditions and methodology of the application of Raman microspectrometry to gaseous, aqueous and hydrocarbon inclusions, and their daughter minerals.     

The structure and sequence of geological events in the Basement Complex of the Ibadan area,Western Nigeria

The Basement Complex in Western Nigeria in general, and in the Ibadan area in particular, is composed primarily of a banded gneiss in which hornblende-biotite rich bands alternate with quartz-oligoclase rich bands. The banded gneiss, which originated as part of a sedimentary sequence, contains large lenses of granite gneiss and thin intercolated layers of quartzite and amphibolite.Two distinct major structural events can be clearly identified in the early geological history of the Ibadan area. Detailed field studies suggest that the formation of the Ibadan Granite Gneiss, which had yielded an Eburnean Rb-Sr isochron age, was associated with the later of these events. Five phases of dyke or vein formation, two of which pre-date the formation of the Granite Gneiss, have also been identified giving an overall sequence of geological events the first of which may correspond to the beginning of the Liberian orogeny, around 3000 m.y. ago, and the last of which reflects the waning of the Pan-African thermo-tectonic event about 500 m.y. ago.     

Denitrification and N2O production in near-shore marine sediments

Methods were developed for determining rates of denitrification in coastal marine sediments by measuring the production of N₂ from undisturbed cores incubated in gas-tight chambers. Denitrification rates at summer temperatures (23°C) in sediment cores from Narragansett Bay, Rhode Island, were about 50μmol N₂m^?2 hr^?1. This nitrogen flux is equal to approximately one-half of the NH⁺₄flux from the sediments at this temperature and is of the magnitude necessary to account for the anomalously low N/P and anomalously high O/N ratios often reported for benthic nutrient fluxes. The loss of fixed nitrogen as N₂ during the benthic remineralization of organic matter, coupled with the importance of benthic remineralization processes in shallow coastal waters may help to explain why the availability of fixed nitrogen is a major factor limiting primary production in these areas. Narragansett Bay sediments are also a source of N₂O, but the amount of nitrogen involved was only about 0.2 μmol m^?2 hr^?1 at 23°C.     

Neogene north American-Caribbean plate boundary across Northern Central America: Offset along the polochic fault

The Polochic fault was a segment of the North American-Caribbean plate boundary across Central America in the Neogene. Its 130 km of left slip was previously determined by matching structures and stratigraphie outcrop patterns of northwest and central Guatemala across the fault. Additional support for the model and the youthfulness of the recorded offset comes from an essentially perfect match of major geomorphic features across the fault. A reconstruction process which eliminates 123 km of left slip brings together rivers and drainage divides that existed before the Polochic became active.With the reconstruction carried across the isthmus on an east-west fault the regional structural geology assumes the coherent pattern of a continuous orogenic belt whose geometry is compatible with the model of collisional tectonics centered on the Motagua “suture zone”. Confined within this belt, narrowed to some 60 km by the reconstruction, lie the major Laramide thrusts, folds and tectonically emplaced serpentinites of Guatemala. Crystalline rocks of Guatemala re-join the Chiapas Massif and Paleozoic sedimentary rocks, exposed in the core of an almost-continuous anticlinorium, extend from southern Chiapas to Lake Izabal.The Polochic does not bend in eastern Guatemala but continues eastward to the Motagua fault where it dies. Westward drift of the northern block resulted in rifting which extended from eastern Guatemala into the Caribbean along the Cayman trough. The Honduras depression may represent an element of a triple junction along with the Polochic and Izabal-Cayman rift.The Polochic continues westward into the Pacific Ocean and offsets the Middle America trench. The Polochic has offset the Miocene volcanic belt of northern Central America, confirming the previous estimate of a Neogene time of movement.About 300 km of relative east-west Neogene displacement has been recorded on the Mid-Cayman rise, only 130 km of which can be accounted for across the Polochic. It is suggested that cumulative extension on north-south faults south of the Motagua fault zone between the trench and the Honduras depression might make up that difference.     

Nitrogen-bearing,aqueous fluid inclusions in some eclogites from the Western Gneiss Region of the Norwegian Caledonides

Minerals in eclogites from different localities in the Western Gneiss Region of the Norwegian Caledonides (age 425 Ma) contain a variety of fluid inclusions. The earliest inclusions recognized are contained in undeformed quartz grains, protected by garnet, and consist of H₂O+N₂ (with ). The reconstructed P-V-T-X properties of these fluid inclusions are compatible with peak or early-retrograde metamorphic conditions. Matrix minerals (quartz, garnet, apatite, plagioclase) contain a complex pattern of mostly truly secondary inclusions, dominated by CO₂ and N₂. The textural patterns and P-V-T-X properties of these inclusions are incompatible with the high pressures of the eclogite-forming metamorphic event, but suggest that they were formed during uplift, by a combination of remobilization of preexisting inclusions and influx of external fluids. The fluid introduced at a late stage was dominated by CO₂, and did not contain N₂. The present data agree with theoretical predictions of eclogite fluids from mineral equilibria, and highlight the differences between granulite (CO₂) and eclogite (H₂O+N₂) fluid regimes. The provenance of the nitrogen in the eclogite fluid inclusions represents an important, but unsolved question in the petrology of high-pressure metamorphic rocks.Contribution no. 68 to the Norwegian programme of the International Lithosphere Project