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41.
GSTA is a major glutathione S-transferase gene responsible for 4-hydroxynonenal conjugation in largemouth bass liver 总被引:2,自引:0,他引:2
We have previously shown that largemouth bass (Micropterus salmoides) has a remarkable ability to conjugate 4-hydroxy-2-nonenal (4HNE), a mutagenic and cytotoxic alpha,beta-unsaturated aldehyde produced during the peroxidation of lipids. In addition, we have isolated a glutathione S-transferase cDNA (bass GSTA) that encodes a recombinant protein which is highly active in 4HNE conjugation and structurally similar to plaice (Pleuronectes platessa) GSTA. In the present study, HPLC-GST subunit analysis revealed the presence of at least two major GST isoforms in bass liver, with one peak constituting 80% of the total bass liver GST protein. Liquid chromatography mass spectrometry (LC-MS) and electrospray ionization analysis of the major bass GST subunit yielded a molecular weight of 26,396 kDa. Endo-proteinase Lys-C digestion and Edman degradation protein sequencing of this GST peak demonstrated that this protein was encoded by bass GSTA. Analysis of genomic DNA fragments isolated by nested PCR indicated the presence of a GST gene cluster in bass liver that contained GSTA, and was similar to a GST gene cluster characterized by Leaver et al., in plaice. Collectively, our data indicates the presence of a major GST in bass liver involved in the protection against oxidative stress. This GST is part of a gene cluster that may be conserved in certain freshwater and marine fish. 相似文献
42.
??????????????????????о??????????????????е?Ч??,???????????????????????????????????????????????????????о????????:1)????????????????????????????о??????????????????Ч??????????????;2)??????????????????Ч?о?????????????????????????????????????????п????;3)??????????????????????????????????????????????????????????? 相似文献
43.
This study examines the roles of the multi-physics approach in accounting for model errors for typhoon forecasts with the local ensemble transform Kalman filter (LETKF). Experiments with forecasts of Typhoon Conson (2010) using the weather research and forecasting (WRF) model show that use of the WRF’s multiple physical parameterization schemes to represent the model uncertainties can help the LETKF provide better forecasts of Typhoon Conson in terms of the forecast errors, the ensemble spread, the root mean square errors, the cross-correlation between mass and wind field as well as the coherent structure of the ensemble spread along the storm center. Sensitivity experiments with the WRF model show that the optimum number of the multi-physics ensemble is roughly equal to the number of combinations of different physics schemes assigned in the multi-physics ensemble. Additional idealized experiments with the Lorenz 40-variable model to isolate the dual roles of the multi-physics ensemble in correcting model errors and expanding the local ensemble space show that the multi-physics approach appears to be more essential in augmenting the local rank representation of the LETKF algorithm rather than directly accounting for model errors during the early cycles. The results in this study suggest that the multi-physics approach is a good option for short-range forecast applications with full physics models in which the spinup of the ensemble Kalman filter may take too long for the ensemble spread to capture efficiently model errors and cross-correlations among model variables. 相似文献
44.
Blubber samples of Baikal seals pups (Phoca sibirica) collected in 2001 and 2002 were analyzed for persistent organochlorines (OCs) such as PCBs (polychlorinated biphenyls), DDTs (dichlorodiphenyltrichloroethane and its metabolites), HCHs (hexachlorocyclohexane isomers), CHLs (chlordane related compounds), TCPMe [tris(4-chlorophenyl)methane] and TCPMOH [tris(4-chlorophenyl)methanol]. The distribution pattern of OCs was in the following order: DDTs>PCBs>CHLs>HCHs>TCPMOH>TCPMe. Organochlorine concentrations and composition patterns in eight pup blubber samples (2002) analyzed in the present study were compared with the corresponding levels in juvenile Baikal seals from 1992 survey, and it was found that residue levels of OC compounds have declined during the 10 year period. In particular, concentrations of PCBs, HCHs and CHLs in seals collected in 2002 were approximately two times lower, while DDTs levels were three times lower than the corresponding levels in 1992. Furthermore, higher ratios of beta-HCH/HCHs and p,p(')-DDE/DDTs were found in 2002 samples. While comparing the OC levels with those in other seal pups it appeared that all the OC concentrations were lower than the levels in seals from coastal California known as one of the most polluted areas. PCB and DDT concentrations in the present specimens were higher than that in Arctic seal pups. The proportions of tri-, tetra-, penta- and hexachlorobiphenyls in pups captured in 2002 were higher than those in 1992. TEQ concentrations of non- and mono-ortho coplanar PCBs in Baikal seals collected in 2002 were higher than those in seals collected from some locations in the world, where human and industrial activities are extensive, suggesting potential for toxic implications of coplanar PCBs in Baikal seals. 相似文献
45.
Binh Thai Pham Abolfazl Jaafari Tran Van Phong Hoang Phan Hai Yen Tran Thi Tuyen Vu Van Luong Huu Duy Nguyen Hiep Van Le Loke Kok Foong 《地学前缘(英文版)》2021,12(3):101105
Improving the accuracy of flood prediction and mapping is crucial for reducing damage resulting from flood events. In this study, we proposed and validated three ensemble models based on the Best First Decision Tree (BFT) and the Bagging (Bagging-BFT), Decorate (Bagging-BFT), and Random Subspace (RSS-BFT) ensemble learning techniques for an improved prediction of flood susceptibility in a spatially-explicit manner. A total number of 126 historical flood events from the Nghe An Province (Vietnam) were connected to a set of 10 flood influencing factors (slope, elevation, aspect, curvature, river density, distance from rivers, flow direction, geology, soil, and land use) for generating the training and validation datasets. The models were validated via several performance metrics that demonstrated the capability of all three ensemble models in elucidating the underlying pattern of flood occurrences within the research area and predicting the probability of future flood events. Based on the Area Under the receiver operating characteristic Curve (AUC), the ensemble Decorate-BFT model that achieved an AUC value of 0.989 was identified as the superior model over the RSS-BFT (AUC = 0.982) and Bagging-BFT (AUC = 0.967) models. A comparison between the performance of the models and the models previously reported in the literature confirmed that our ensemble models provided a reliable estimate of flood susceptibilities and their resulting susceptibility maps are trustful for flood early warning systems as well as development of mitigation plans. 相似文献
46.
He Ziguang Nguyen Hoang Vu Thai Ha Zhou Jian Asteris Panagiotis G. Mammou Anna 《Acta Geotechnica》2022,17(4):1257-1272
Acta Geotechnica - Soft soils are considered as disadvantages in construction, especially in clay layers. It requires many advanced techniques to treat the soft soils before construction, aiming to... 相似文献
47.
Mathematical Geosciences - 相似文献
48.
本研究项目是采用诸如地质学解释、影像判读和地球物理探测等多种方法相结合进行的。在所得结果的基础上参考了越南国内外同行的一些资料编制了东南亚、越南及邻区的断裂构造图.其比例尺分别为1:4百万和1:1百万。分析所得结果显示出东南亚断裂构造演化的下列情况:1)在现今地质结构方面东南亚是欧亚岩石圈板块的东南部分.由一条消减带围绕.这条消减带的伸展从Myanmer开始,通过Nicobar,Java Timor直到东菲律宾。东南亚被Song Hong(即红河)断裂,Three Pagodas断裂和Hainam-Natuna断裂等2级断裂系统分成3个微板块。2)在早新生代.东南亚是分为5个微板块的。它们的分界断裂中有2个一级断裂(中央东海扩张带和Lupar-Kuching消减带)和3个二级断裂(即上述3个)。3)上述绝大多数二和三级断裂从晚新生代起活化且继承了从早新生代即已发生和发展了的二、三级断裂,但在某些条件下.运动方向却完全变成了相反,尤其是走滑运动的方向。我们的研究结果表明:在这一地区内,盆地、隆起、岩浆侵入、褶皱和局部断裂等构造的形成都取决于这些沿着一、二级走滑断裂的微板块运动。 相似文献
49.
Zhu Yulong Ishikawa Tatsuya Zhang Yafen Nguyen Binh T. Subramanian Srikrishnan Siva 《Landslides》2022,19(8):2021-2032
Landslides - Limited by the independence and its defects of each general software package, simultaneous analysis of runoff, seepage, and large-deformation analysis is still an inevitable challenge.... 相似文献
50.
Peter S. Fiske Charles C. Schnetzler John Mchone Kong Kham Chanthavaichith Inpong Homsombath Thonkeo Phouthakayalat Bounsou Khenthavong Pham Tich Xuan 《Meteoritics & planetary science》1999,34(5):757-761
Abstract— We have recovered 18 kg of layered tektites from 10 tektite-bearing localities in Laos and central Vietnam, including 5 localities around the town of Muong Nong (Laos). Several of these deposits originally contained several hundred kilograms of layered tektite fragments, and one fragmented mass may have been as large as 1000 kg. This is the largest single deposit of tektites yet reported. In this region, layered tektite fragments are found in isolated clusters usually associated with a pebbly laterite horizon that is 0–1 m below the surface. Near Khe Sanh, Vietnam, we estimate the abundance of layered tektite fragments to be ~100 g/m2. This is greater than five times the abundance estimated for northeast Thailand (Fiske et al., 1996). In a region that extends from northeast Thailand, through central Laos, and into central Vietnam, we found only layered tektites, which confirmed the existence of a large (>50 000 km2) subfield of the Australasian strewn field with only layered tektites. The east-west extent of the “layered-only” subfield is well constrained, but little field data exist to constrain its north-south extent. 相似文献