Enzymatic hydrolysis of defatted mackerel protein with low bitter taste

Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain protein hydrolysates with high ni-trogen recovery and low bitter taste,enzymatic hydrolysis was performed using eight commercially available proteases.It turned out that the optimum enzyme was the ‘Mixed enzymes for animal proteolysis’.An enzyme dosage of 4%,a temperature of 50℃,and a hydrolysis time of 300 min were found to be the optimum conditions to obtain high NR (84.28%) and degree of hydrolysis (DH,16.18%) by orthogonal experiments.Glutamic acid was the most abundant amino acid of MDP (defatted mackerel protein) and MDPH (defatted mackerel protein hydrolysates).Compared with the FAO/WHO reference protein,the essential amino acid chemical scores (CS) were greater than 1.0 (1.0 1.7) in MDPH,which is reflective of high nutritional value.This,coupled with the light color and slight fishy odor,indicates that MDPH would potentially have a wide range of applications such as nutritional additives,functional ingredients,and so on.     

Tests of new in-situ seabed acoustic measurement system in Qingdao

A new in-situ seabed acoustic measurement system is developed for direct in-situ measurement of sediment geoacoustic properties （compressional wave velocity and attenuation）. The new in-situ system consists of two parts： the deck control unit and the underwater measurement unit. The underwater measurement unit emits sonic waves that propagate through the seafloor sediment, receives the returning signals, and transmits them to the deck control unit for waveform display and analysis. The entire operation is controlled and monitored in real time by the deck control unit on the research vessel and can provide recording of full waveforms to determine the sound velocity and attenuation. This paper outlines the design of the system, the measurement process, and demonstrates its application in tests carded out on seabed sediment off the Qingdao coast, China. The test results show that the system performed well and rapidly provided accurate in-situ acoustic velocity and attenuation estimates of the seafloor sediment.     

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Short- and long-term response of phytoplankton to ENSO in Prydz Bay,Antarctica: Evidences from field measurements,remote sensing data and stratigraphic biomarker records

The study provides one of the first lines of evidence showing linkages between Antarctic phytoplankton abundance and composition in response to ENSO, based on historical reconstruction of sediment biomarkers. In addition to sediment biomarkers, field measured and remote sensing data of phytoplankton abundance were also recorded from Prydz Bay, Eastern Antarctica. Com-munity structure of field measured phytoplankton showed significant El Ni？o/La Ni？a-related succession during 1990 to 2002. In general, the number of algae species decreased during El Ni？o and La Ni？a years compared to normal years. Austral summer monthly variation of remotely sensed chlorophyll-a （Chl-a）, particulate organic carbon （POC）, and sea surface temperature （SST） indicated that ENSO impacted the timing of phytoplankton blooms during 2007 to 2011. Phytoplankton blooms （indicated by Chl-a and POC） preceded the increases in SST during El Ni？o years, and lagged behind the SST increases during La Ni？a years. Stratigraphic record of marine sedimentary lipid （brassicasterol, dinosterol and alkenones） biomarkers inferred that the proportions of different algae （diatoms, dinoflagellates and haptophytes） changed significantly between El Ni？o and La Ni？a events. The relative proportion of diatoms increased, with that of dinoflagellates being decreased during El Ni？o years, while it was reversed during La Ni？a years.     

Broader pattern of tandem repeats in the mitochondrial control region of Perciformes

Perciformes, the largest order of vertebrates with 20 suborders, is the most diverse fish order that dominates vertebrate ocean life. The complete mitochondrial control region (CR) of Trichiurus japonicus (Trichiuridae, Scombroidei) and Pampus sp. (Stromateidae, Stromateoidei) were amplified and sequenced. Together with data from GenBank, the tandem repeats in the mitochondrial CR from 48 species, which covered nine suborders of Perciformes, are reported in this study. The tandem repeats tend to be long in the suborder Percoidei and Stromateoidei. The identical repeats in 21 species of Cichlidae suggest a common origin and have existed before species divergence. Larimichthys crocea shows tandem repeats instead of the typical structure of the central conserved sequence blocks, which was first reported in Perciformes and vertebrates. This might have resulted from interruption of the polymerase activity during the H-strand synthesis. The four broader patterns presented here for the tandem repeats, including those in both the 5′ and 3′ ends, only in the either 5′ or 3′ end, and in the central conserved domain of the control region, will be useful for understanding the evolution of species.     

Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification

Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65°C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.     

基于模糊分类的UBGM(1,1)-Markov模型在露天矿边坡沉降预测中的应用

针对UBGM(1,1)-Markov模型中存在2个邻近值可能被归属到不同状态,导致预测值产生偏差的问题,结合模糊分类理论,构建基于模糊分类的无偏灰色-马尔科夫模型(unbiased gray-Markov model based on fuzzy classification, FC-UBGM(1,1)-Markov)。首先对UBGM(1,1)模型进行残差修正,然后将修正后拟合值的相对残差序列作为Markov链进行区间划分,再结合模糊分类的隶属度函数,计算相对残差的模糊向量,根据隶属度确定其所属的状态。实际算例表明,该模型比传统UBGM(1,1)-Markov模型的预测效果更好。     

Effect of stocking density on water quality and (Growth,Body Composition and Plasma Cortisol Content) performance of pen-reared rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>)

The goal of the study was to examine the effect of stocking density on the water quality of culture area, as well as the growth, body composition and cortisol content of rainbow trout（Oncorhynchus mykiss）. Pen-reared trout were stocked in densities of 40, 60, 80 fish individuals m^-3(4.6, 6.6, 8.6 kg m^-3, SD1, SD2 and SD3 groups, respectively) for 300 days. Compared to the water from SD1 and SD2, that from SD3 exhibited significantly higher NH₄⁺-N content and COD（chemical-oxygen-demand）, and a significant reduction of dissolved oxygen in day 180(40.6 kg m^-3). Stocking density was significantly associated with body weight, standard length, VSI（viscerosomatic index）, CF（condition factor） and FC（food coefficient） in group SD3, particularly in day 240 and day 300(45 or 49.3 kg m^-3). Increased crude fat and decreased crude protein were displayed in high density group when the density reached to 36 kg m^-3. As a cumulative effect of density-related stress, VSI, CF, FC, moisture, and crude protein content varied over time in each density group（SD1, SD2, and SD3）. In summary, trout exhibited a better growth performance in low density(26.3 kg m^-3) than those reared in high densities(36 and 45 kg m^-3). The results indicate that rainbow trout（114.44 g ± 6.21 g, 19.69 cm ± 0.31 cm） initially stocked in 6.6 or 8.6 kg m^-3 should be lightened to less than 36 kg m^-3 after an intensive rearing for 240 days.     

Research progress of TiO2 photocathodic protection to metals in marine environment

Corrosion protection has become an important issue as the amount of infrastructure construction in marine environment increased.Photocathodic protection is a promising method to reduce the corrosion of metals,and titanium dioxide(TiO_2) is the most widely used photoanode.This review summarizes the progress in TiO_2 photo gene rated protection in recent years.Different types of semiconductors,including sulfides,metals,metal oxide s,polymers,and other materials,are used to design and modify TiO_2.The strategy to dramatically improve the efficiency of photoactivity is proposed,and the mechanism is investigated in detail.Characterization methods are also introduced,including morphology testing,light absorption,photoelectrochemistry,and protected metal observation.This review aims to provide a comprehensive overview of Ti02 development and guide photocathodic protection.     

Provenance and Tectonic Implications of Paleozoic Strata in the South Yellow Sea Basin,China–Revealed from the Borehole CSDP-2

Well CSDP-2 is a fully coring deep drilling hole, penetrating the thick Paleozoic marine strata in the South Yellow Sea Basin(SYSB) in the lower Yangtze Block(LYB). Based on the petrological and geochemical analysis of 40 sandstone samples from the core CSDP-2, the provenance and tectonic features of Paleozoic detrital rocks from SYSB are analyzed and systematically delineated in this article. The results show that the Silurian – Carboniferous sandstones are mature sublitharenite, while the Permian sandstones are unstable feldspathic litharenite. The average CIA(chemical index of alteration) is 74.61, which reflects these sediments were derived from source rocks with moderate chemical weathering. The REE(rare earth element) patterns are characterized by LREE enrichment, flat HREE and obviously negative Eu anomaly, which are similar to that of the upper continental crust. Dickinson QFL discrimination results indicate the recycled orogeny provenance. Various diagrams for the discrimination of sedimentary provenance based on major and trace element data show all the sediments were derived predominantly from quartz sedimentary rocks, of which the Permian strata contain more felsic sedimentary rocks. Geochemical data for these detrital rocks suggest they occur at the passive continental margin and island arc settings, and the Permian sandstone presents active continental margin setting.