Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

冲绳海槽中西部边缘区沉积物成因和沉积环境

本文根据１９９６年“中－法合作东海计划”在冲绳海槽中西部边缘区所取柱状样岩芯沉积物粒度分析资料,对该区沉积物特征进行研究。结果表明,火山沉积和富含生物的半远洋深海软泥沉积是本区的主要特征。以４８１ｃｍ为界,上、下两层沉积物截然不同,可能是沉积物的来源不同或者是水动力条件的差异。该区主要受火山活动的影响,其次是黑潮、大洋风暴     

Summer Arctic sea fog

I~IOXSea fog is a kind of dangerous weather. Chinese sea fog experts, Wang Binhua (1983),Hu Ruijin and Zhou Faxiu (1998) and Hu Jifu et al. (1996) studied sea fog rather Systematically. FOreign Experts also Paid great attention to sea fog. Ernlnons and Montgomery(1974), chipper (1994) and Rayrnond et al. (1989) have studied sea fog thorOUghly.HOwever, studies on Arctic sea ice have rarely been carried Out becauSe of the sever environment and less htnnan activity in the region. There …     

影响萼花臂尾轮虫种群增长的生态学因子的研究：温度和饵料密度的影响

本文研究了在5个温度梯度和4个饵料密度的20个试验组合下，萼化臂尾轮虫（Brachionuscalyciflorus）的种群增长规律。结果表明，该轮虫实验种群的最适温度范围是28—32℃，临界高温是36－40℃；最适饵料密度是45－65×106个／ml。这两个生态因子的最佳组合是温度32℃，饵料密度为65×106个／ml，此时，种群达最大密度3．4×103个／ml。     

一个湍能封闭模型对风暴潮海底底应力的应用研究

将一个三维湍能封闭模型应用于开阔海区的风暴潮，通过数值计算探讨了Ｔａｙｌｏｒ底摩擦二次率的拖曳系数随空间的分布及拖曳系数与水深、海底粗糙度、风向和风速等因素的关系。本文对底摩擦二次率的可靠性做了评价。     

A new type of Shipboard Meteorological Satellite Receiving-processing System

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…     

大生活用海水生化处理技术研究进展

采用海水作为大生活用水最结解沿海城市淡水资源紧缺局面的有效途径之一。该技术得以推广应用的技术关键是大生活用海水的后处理技术，文章综述了大生活用海水的生化处理技术的研究现状，并提出了今后的研究方向。     

碳水化合物的组合合成

碳水化合物的组合合成是一项新兴技术，该技术可以在短时间内合成大量用于进行生物活性筛选的寡糖及拟糖物。液相与固相合成技术可以极大地加快药物研究与开发进程。作者针对该技术在碳水化合物合成方面的研究进展情况进行讨论。     

黄海区水母类的生态研究

本文根据１９８２年４月２１日～５月１２日，７月２３日～８月５日，１０月１２～３０日在３４°３０′Ｎ以北．１２４°Ｅ以西黄海水域污染现状综合调查中获得的样品和资料，分析了本海区水母类的种类组成，数量分布以及季节变化。调查海区共鉴定有３４种水母，其中优势种有盘形美螅水母Ｃｌｙｔｉａｄｉｓｃｏｉｄａ，四枝管水母Ｐｒｏｂｏｓｃｉｄａｃｔｙｌａｆｌａｖｉｃｉｒｒａｔａ，五角水母Ｍｕｇｇｉａｅａａｔｔａｎｔｉｃａ。秋季（１０月）为水母类总个体数的高峰期（８．６７个／ｍ３）。水母类的分布与水温、盐度密切相关，海流或水团也起重要作用．水母类分布的变更可以反映出沿岸水和外海水相互推移和消长状况。     

植物硅酸体的研究及在我国第四纪地质学中的初步应用

本文简述了国外植物硅酸体(植物蛋白石)这门新兴学科的进展情况,介绍了植物硅酸体研究在我国第四纪地质学中如古气候与古环境、地层时代、考古等方面的初步应用。