Fish Zona radiata (eggshell) proteins: evaluation and validation as a biomarker for xenoestrogen monitoring

New cellular and molecular tools are enabling researchers to study the basic mechanisms of action of natural reproductive hormones, and of endocrine disrupters, including xenoestrogens. Zonagenesis is defined by the hepatic synthesis of the eggshell Zona radiata proteins (Zrp), their secretion and transport in the blood and their uptake by maturing oocytes. Zrps are comprised of three-to-four protein monomers in the 50–60 kDa MW range and show structural similarity to proteins of the Zona pellucida; an extracellular envelope that lies immediately outside the plasma membrane in the eggs of placental mammals. We studied the xenoestrogen-induced elevation in hepatic mRNA and plasma levels of Zrp and vitellogenin (Vtg) in several fish species. Eggshell Zrp protein and mRNA was induced in juvenile salmon by exposure to nonylphenol (NP: given singly or in combination with other xenoestrogens/xenobiotics). In English sole (Pleuronectes vetulus), Zrp was induced in the plasma of mature female, E₂- and NP-treated male fish. We are currently using salmon cDNA probes to study estrogen-receptor, Zrp and Vtg mRNA expression in English sole treated with E₂- and NP. Validation in a semi-field experiment showed that effluent from an oil refinery treatment plant caused a dose-dependent induction of Zrp and Vtg in juvenile salmon. However, a component corresponding to Zrp-β was more responsive than Zrp-α and Zrp-γ and also more than Vtg. Zrp seem to be a sensitive complement to Vtg analysis, and appear to be a more ecologically significant biomarker of estrogenicity.     

Australian ‘gayborhoods’ and ‘lesborhoods’: a new method for estimating the number and prevalence of adult gay men and lesbian women living in each Australian postcode

ABSTRACT

Gay men and lesbian women often demonstrate unique settlement patterns, forming what have been referred to as ‘gayborhoods’. This study sought to provide the first postcode-level estimates of population size and prevalence of gay and lesbian people in Australia. Data on same-gender-partnered households from the Australian Census were combined with information from six different surveys conducted from 2011 to 2017. We estimated that in 2016 there were 132,203 gay men (1.5% of adult males; 95% CI: 1.4–1.6) and 79,931 lesbian women (0.9% of adult females; 95% CI: 0.8–1.0) in Australia. While many postcodes were sparsely populated by gay and lesbian people (40.1% had prevalences of <0.1%), 24.6% were moderately populated (prevalences in the 50-95th percentile) and 2.7% were highly populated (95th percentile). By jurisdiction, the Australian Capital Territory had the highest prevalences of gay men (2.1%; 95% CI: 2.0–2.2) and lesbian women (1.5%; 95% CI: 1.4–1.6). Although the majority of highly populated postcodes were found in major cities (83.7%), some were also found in regional and remote area (16.3%). This method can be applied in other countries to enhance populate estimates. The accompanying dataset can be used to guide service delivery, conduct geographically contextualised research and develop policies relevant to gay men and lesbian women in Australia.     

Phytate as a novel phosphorus-specific paleo-indicator in aquatic sediments

A reliable geochemical paleo-indicator for phosphorus remains elusive, despite the importance of understanding historical changes in the nutrient status of aquatic ecosystems. We assessed the potential of phytate (salts of myo-inositol hexakisphosphate) as a novel phosphorus-specific paleo-indicator by measuring its concentrations in dated sediments from an embayment in Helsinki, Finland, with a known 200-year history of trophic changes. Phytate was extracted in a solution containing sodium hydroxide and EDTA and detected by solution ³¹P NMR spectroscopy with spectral deconvolution. Concentrations varied markedly with sediment depth and paralleled previously determined changes in diatom assemblages and geochemical indicators linked to trophic status. In contrast, total sediment phosphorus did not reflect phosphorus inputs to the embayment, presumably due to the mobilization of inorganic phosphate under anoxic conditions during periods of high pollutant loading. Importantly, phytate appeared to be stable in these brackish sediments, in contrast to other organic and inorganic phosphates which declined abruptly with depth. We therefore conclude that phytate represents a potentially important indicator of historical changes in phosphorus inputs to water bodies, although additional studies are required to confirm its stability under conditions likely to be encountered in lakes and coastal ecosystems.     

Trapped Ar isotopes in meteorite ALH 84001 indicate Mars did not have a thick ancient atmosphere

Water is not currently stable in liquid form on the martian surface due to the present mean atmospheric pressure of ～7 mbar and mean global temperature of ～220 K. However, geomorphic features and hydrated mineral assemblages suggest that Mars’ climate was once warmer and liquid water flowed on the surface. These observations may indicate a substantially more massive atmosphere in the past, but there have been few observational constraints on paleoatmospheric pressures. Here we show how the ⁴⁰Ar/³⁶Ar ratios of trapped gases within martian meteorite ALH 84001 constrain paleoatmospheric pressure on Mars during the Noachian era [～4.56–3.8 billion years (Ga)]. Our model indicates that atmospheric pressures did not exceed ～1.5 bar during the first 400 million years (Ma) of the Noachian era, and were <400 mbar by 4.16 Ga. Such pressures of CO₂ are only sufficient to stabilize liquid water on Mars’ surface at low latitudes during seasonally warm periods. Other greenhouse gases like SO₂ and water vapor may have played an important role in intermittently stabilizing liquid water at higher latitudes following major volcanic eruptions or impact events.     

Ecohydrographic constraints on biodiversity and distribution of phytoplankton and zooplankton in coral reefs of the Red Sea,Saudi Arabia

An integral concept of ecological research is the constraint of biodiversity along latitudinal and environmental gradients. The Red Sea features a natural example of a latitudinal gradient of salinity, temperature and nutrient richness. Coral reefs along the Red Sea coasts are supported with allochthonous resources such as oceanic and neritic phytoplankton and zooplankton; however, relatively little is known about how the ecohydrography correlates with plankton biodiversity and abundance. In this article we present the biodiversity of phytoplankton and zooplankton in Red Sea coral reefs. Oceanographic data (temperature, salinity), water samples for nutrient analysis, particulate organic matter, phytoplankton and zooplankton, the latter with special reference to Copepoda (Crustacea), were collected at nine coral reefs over ~1500 km distance along the Red Sea coast of Saudi Arabia. The trophic state of ambient waters [as indicated by chlorophyll a (Chl a)] changed from strong oligotrophy in the north to mesotrophy in the south and was associated with increasing biomasses of Bacillariophyceae, picoeukaryotes and Synechococcus as indicated by pigment fingerprinting (CHEMTAX) and flow cytometry. Net‐phytoplankton microscopy revealed a Trichodesmium erythraeum (Cyanobacteria) bloom north of the Farasan Islands. Several potentially harmful algae, including Dinophysis miles and Gonyaulax spinifera (Dinophyceae), were encountered in larger numbers in the vicinity of the aquaculture facilities at Al Lith. Changes in zooplankton abundance were mainly correlated to the phytoplankton biomass following the latitudinal gradient. The largest zooplankton abundance was observed at the Farasan Archipelago, despite high abundances of copepodites, veligers (Gastropoda larvae) and Chaetognatha at Al Lith. Although the community composition changed over latitude, biodiversity indices of phytoplankton and zooplankton did not exhibit a systematic pattern. As this study constitutes the first current account of the plankton biodiversity in Red Sea coral reefs at a large spatial scale, the results will be informative for ecosystem‐based management along the coastline of Saudi Arabia.     

Simulated effects of land immersion on regional arid climate: a case study of the pre-Saharan playa of Chott el-Jerid (south of Tunisia)

The potential effects on the regional climate induced by partially immersing the arid pre-Saharan playa basin of Chott el-Jerid (south of Tunisia) are investigated by comparing two multi-year (1991–2011) sets of numerical simulations each consisting of ten-member ensemble and performed using the WRF regional climate model. The first WRF ensemble is performed under current land use and land cover, while the second is carried out after introducing a virtual large and shallow surface water reservoir (a lake) in Chott el-Jerid. The most pronounced effects generated by the artificial lake are circumscribed over its surface and slightly spread downwind to the other parts of the Chott. The lake has a clear moderating effect on near-surface air temperatures by increasing (decreasing) the wintertime (summertime) air temperatures. Sensible heat fluxes are remarkably increased in winter and decreased in summer over the lake following the temperature gradient between the lake surface and the overlying atmosphere. Latent heat fluxes, moisture convergence, and water vapor mixing ratio are increased over the lake throughout the year, especially in winter. The lake also induces domain-wide decreased (increased) surface pressures and land (lake) breeze circulation in winter (summer). Simulated rainfall amounts are most increased over the lake in winter likely because of an enhanced atmospheric instability, while they slightly decrease in summer.     

Mapping benthic habitat in regions of gradational substrata: An automated approach utilising geophysical,geological, and biological relationships

The relationship between acoustic backscatter, sediment characteristics and benthic habitat is examined using high-resolution sidescan sonar data collected at the Loch Linnhe artificial reef site on the west coast of Scotland. The site is typical for the continental shelf of NW Europe, with a mix of seabed environments from muddy to coarse, stony substrata on a 10–100 m length scale. A sidescan sonar mosaic was produced and classified according to derived backscatter parameters (mean, median and standard deviation of the backscatter values) using an unsupervised classification procedure. The accuracy of the final classified map was assessed by comparison with a ground-truthing survey in which the biological habitat was derived from underwater video footage. The sidescan correctly predicted seabed surface characteristics of observed biological habitat with 78% accuracy. A second, and more challenging test of the acoustic data to correctly predict biological habitat was made by comparing it with data from 21 grab sampling stations. These stations were divided into three groups using multivariate statistical techniques based on their backscatter properties. Benthic assemblage structure was found to be significantly distinct between the high and low, and the medium and low backscatter stations. There was a low to moderate but significant correlation between the multivariate patterns of acoustic backscatter, benthic assemblage structure, and particle size distribution. The work shows that even in areas with subtle and gradational changes in substratum, the sidescan was able to predict biological community with an acceptable accuracy.     

Earth-Affecting Solar Causes Observatory (EASCO): A potential International Living with a Star Mission from Sun–Earth L5

This paper describes the scientific rationale for an L5 mission and a partial list of key scientific instruments the mission should carry. The L5 vantage point provides an unprecedented view of the solar disturbances and their solar sources that can greatly advance the science behind space weather. A coronagraph and a heliospheric imager at L5 will be able to view CMEs broadsided, so space speed of the Earth-directed CMEs can be measured accurately and their radial structure discerned. In addition, an inner coronal imager and a magnetograph from L5 can give advance information on active regions and coronal holes that will soon rotate on to the solar disk. Radio remote sensing at low frequencies can provide information on shock-driving CMEs, the most dangerous of all CMEs. Coordinated helioseismic measurements from the Sun–Earth line and L5 provide information on the physical conditions at the base of the convection zone, where solar magnetism originates. Finally, in situ measurements at L5 can provide information on the large-scale solar wind structures (corotating interaction regions (CIRs)) heading towards Earth that potentially result in adverse space weather.     

A model for oxygen and sulfur isotope fractionation in sulfate during bacterial sulfate reduction processes

We present a model of bacterial sulfate reduction that includes equations describing the fractionation relationship between the sulfur and the oxygen isotope composition of residual sulfate (δ³⁴S_{SO4_residual}, δ¹⁸O_{SO4_residual}) and the amount of residual sulfate. The model is based exclusively on oxygen isotope exchange between cell-internal sulfur compounds and ambient water as the dominating mechanism controlling oxygen isotope fractionation processes. We show that our model explains δ³⁴S_{SO4_residual} vs. δ¹⁸O_{SO4_residual} patterns observed from natural environments and from laboratory experiments, whereas other models, favoring kinetic isotope fractionation processes as dominant process, fail to explain many (but not all) observed δ³⁴S_{SO4_residual} vs. δ¹⁸O_{SO4_residual} patterns. Moreover, we show that a “typical” δ³⁴S_{SO4_residual} vs. δ¹⁸O_{SO4_residual} slope does not exist. We postulate that measurements of δ³⁴S_{SO4_residual} and δ¹⁸O_{SO4_residual} can be used as a tool to determine cell-specific sulfate reduction rates, oxygen isotope exchange rates, and equilibrium oxygen isotope exchange factors. Data from culture experiments are used to determine the range of sulfur isotope fractionation factors in which a simplified set of equations can be used. Numerical examples demonstrate the application of the equations. We postulate that, during denitrification, the oxygen isotope effects in residual nitrate are also the result of oxygen isotope exchange with ambient water. Consequently, the equations for the relationship between δ³⁴S_{SO4_residual}, δ¹⁸O_{SO4_residual}, and the amount of residual sulfate could be modified and used to calculate the fractionation-relationship between δ¹⁵N_{NO3_residual}, δ¹⁸O_{NO3_residual}, and the amount of residual nitrate during denitrification.