雷诺数对圆形渐缩喷嘴湍流射流的影响

The present study systematically investigates by experiment the influence of Reynolds number (Re) on a turbulent jet issuing from a smoothly-contracting round nozzle. Measurements were performed for seven Reynolds numbers varying from Re = 4,050 to Re = 20,100 using single hot-wire anemometry and over an axial distance of 30 nozzle exit diameters. Although all the exit velocity profiles are of "top-hat" shape, these measurements reveal significant dependence on Re of the exit and downstream flows. The effect of Re on both the mean and turbulent fields is substantial for Re ＜ 10,000 and becomes weak beyond Re = 10,000. The length of the jet’s potential core and the far-field rates of decay and spread all depend significantly on Re.     

Finite element modeling of heating phenomena of cracks excited by high-intensity ultrasonic pulses

A three-dimensional thermo-mechanical coupled finite element model is built up to simulate the phenomena of dynamical contact and frictional heating of crack faces when the plate containing the crack is excited by high-intensity ultrasonic pulses. In the finite element model, the high-power ultrasonic transducer is modeled by using a piezoelectric thermal-analogy method, and the dynamical interaction between both crack faces is modeled using a contact-impact theory. In the simulations, the frictional heating taking place at the crack faces is quantitatively calculated by using finite element thermal-structural coupling analysis, especially, the influences of acoustic chaos to plate vibration and crack heating are calculated and analysed in detail. Meanwhile, the related ultrasonic infrared images are also obtained experimentally, and the theoretical simulation results are in agreement with that of the experiments. The results show that, by using the theoretical method, a good simulation of dynamic interaction and friction heating process of the crack faces under non-chaotic or chaotic sound excitation can be obtained.     

海洋侧孢短芽孢杆菌Lh-1 株所产多肽R-1 性质分析及作用原理探讨

对海洋侧孢短芽孢杆菌(Brevibacillus laterosporus)Lh-1所产多肽R-1的性质及作用机理进行了研究。R-1对温度和pH稳定,在pH 11.0~12条件下,121℃处理1 h,其活力保持在75%以上。经3种不同浓度的蛋白酶(碱性蛋白酶、胰蛋白酶、胃蛋白酶)处理4 h后,活性保持在80%以上。与常见的金属离子和化学试剂在一起反应,对抗菌肽R-1的活性无明显影响,可以配合一起使用,仅吐温和SDS对其影响较大。氨基酸分析表明,抗菌肽R-1由亮氨酸(Leu)、酪氨酸(Tyr)、缬氨酸(Val)、异亮氨酸(Ile)、赖氨酸(Lys)、甘氨酸(Gly)、蛋氨酸(Met)、丝氨酸(Ser)、丙氨酸(Ala)9种氨基酸组成,其中疏水氨基酸居多。抑菌试验表明R-1的抑菌谱广泛,对经培养分离出来的大肠杆菌及白假丝酵母菌的扫描电境超微结构观察表明,R-1的杀菌机制主要是通过含有带正电荷的Lys与带负电荷的磷脂分子结合,然后R-1的疏水端插入到指示菌的疏水区,改变膜的构象,从而导致菌体的死亡。     

产过氧化氢酶菌株CE-1 的筛选与鉴定

经过含H2O2的平板初筛和摇瓶发酵复筛,从保存的水样中筛选到一株过氧化氢酶高产菌株CE-1,其所产过氧化氢酶活性和比活分别为1 356.2 U/mL和3 401 U/mg。菌株CE-1为革兰氏阴性杆状细菌,根据其形态特征、16S rRNA基因序列系统进化树分析和生理生化特性,以及属内不同种间性状差异等因素的综合分析,将菌株CE-1鉴定为气单胞属(Aeromonas)细菌。     

Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

不同类型冷却屋顶方案对城市群热环境的缓解效果

利用耦合单层城市冠层模型的中尺度数值模式WRF/UCM,选取8组不同反照率和绿化比例的屋顶冷却方案进行敏感性试验,模拟研究不同冷却屋顶方案对长三角城市群2013年夏季城市热环境的影响,并分析其影响机制.结果表明:不同冷却屋顶方案对城市群热环境的缓解效果与屋顶参数之间呈很强的线性关系.高温热浪天气下,H R 4(反照率为...     

D—InSAR三轨法监测城市地表沉降的研究

差分干涉测量（DInSAR）技术是合成孔径雷达（SAR）卫星应用的一个拓展。雷达图象的差分干涉可用于监测厘米级或更微小的地表形变,以揭示许多物理现象,如地震形变、火山运动、大气变化、冰川漂移、地面沉降以及山体滑坡等。实验选取典型的上海市地面沉降场作为研究对象,使用三幅欧洲空间局ERS1和ERS2的C波段数据,采用三轨法,成功提取了上海市相隔20个月的地面沉降图。     

壤中气汞测量在活断层研究中的应用

利用JM—3型数字金膜测汞仪对渭河盆地活动断层进行壤中气汞测量,取得了初步结果。认为,壤中气汞异常与活动断层存在着密切的关系,通过测汞寻找隐伏活动断层,判定断层活动性是可行的。     

Ekman motion in shallow open sea in the presence of time-harmonic variation of water depth

The time variation of the wind-induced flow in a homogeneous unbounded sea region has been analytically investigated. The time-dependent Ekman solution in a homogeneous, shallow open sea has been further extended by taking into account the time variation in water depth which might be caused by either tidal motion or change in the mixed layer thickness. The solution approach taken in this study is based on the Galerkin-eigenfunction method originally developed by Heaps [1972. On the numerical solution of three-dimensional hydrodynamical equations for tides and storm surges. Memoires de la Societe Royale des Sciences de Liege Serie 6(2), 143–180]. A series of calculations have been made with emphasis on the influence of the time variation in water depth upon the build-up of Ekman spirals in the presence of oscillatory variations in water depth. It has been found that two oscillations contribute to the wind drift current, the inertial oscillation and the depth-variation-induced oscillation; the inertial oscillation decays with time, but the depth-variation-induced oscillation remains undamped despite the presence of bottom friction. The presence of time-harmonic variation produces peculiar forms of hodograph with a curled or circular pattern according to the angular frequency of the water depth variation.     

3D裂隙网络随机模拟及其工程应用研究

岩体中存在的大量随机分布的裂隙对岩体的工程性质及稳定性有着极其重要的影响。传统的工程地质研究方法难以客观地描述其空间分布特征。应用概率与统计学的理论 ,通过统计分析得到裂隙的分布规律性 ,采用随机模拟的方法实现与统计分布相适应的裂隙网络是研究随机裂隙宏观特征的有效途径。通过模拟所得的 3D裂隙网络 ,进一步研究了裂隙的连通特征及工程开挖面上的稳定分析与加固措施优化的方法