缢蛏糖原磷酸化酶基因多态性与糖原含量的关联分析

为探究缢蛏糖原磷酸化酶基因(Sc-GPH)与糖原含量的关系,本研究克隆获得Sc-GPH cDNA全长,检测其在不同组织、不同月份的表达模式,并在Sc-GPH基因编码区筛选与糖原含量关联的SNP位点.结果表明,Sc-GPH cDNA全长为3963 bp,开放阅读框为2541 bp,编码846个氨基酸;氨基酸序列多重比对和系统发育树显示,缢蛏与欧洲大扇贝、虾夷扇贝、长牡蛎等贝类亲缘关系较近,而与哺乳类、甲壳类、昆虫类亲缘关系较远.实时荧光定量PCR结果显示,Sc-GPH在8个组织中均有表达,其中在外套膜和足中表达量最高(p<0.01),推测与其糖原存储能力有关;不同月份的缢蛏外套膜和足中,Sc-GPH在8月表达量最高,而此时糖原含量较低,说明Sc-GPH的表达可能受缢蛏生殖周期的影响.以缢蛏"甬乐1号"群体为实验材料,在Sc-GPH基因编码区筛选到4个与糖原含量相关SNP位点,其中c.930T>C位点在台州野生群体中得到进一步验证,该位点为高糖原缢蛏分子标记辅助选育提供了候选标记.     

An Improved Time Domain Approach for Analysis of Floating Bridges Based on Dynamic Finite Element Method and State-Space Model

The floating bridge bears the dead weight and live load with buoyancy, and has wide application prospect in deep-water transportation infrastructure. The structural analysis of floating bridge is challenging due to the complicated fluid-solid coupling effects of wind and wave. In this research, a novel time domain approach combining dynamic finite element method and state-space model (SSM) is established for the refined analysis of floating bridges. The dynamic coupled effects induced by wave excitation load, radiation load and buffeting load are carefully simulated. High-precision fitted SSMs for pontoons are established to enhance the calculation efficiency of hydrodynamic radiation forces in time domain. The dispersion relation is also introduced in the analysis model to appropriately consider the phase differences of wave loads on pontoons. The proposed approach is then employed to simulate the dynamic responses of a scaled floating bridge model which has been tested under real wind and wave loads in laboratory. The numerical results are found to agree well with the test data regarding the structural responses of floating bridge under the considered environmental conditions. The proposed time domain approach is considered to be accurate and effective in simulating the structural behaviors of floating bridge under typical environmental conditions.

     

粤港澳大湾区港口供应链优化研究

为充分发挥粤港澳大湾区港口的竞争优势,促进港口经济的协调发展,文章基于粤港澳大湾区主要港口的实际情况,构建供应链博弈模型并进行数值仿真,研究相关因素对深圳港和香港港绩效的影响。研究结果表明:随着商品价格和库存成本的提高,货物从深圳港向香港港转移,但转移幅度逐渐趋缓;深圳港在中低端商品的货源收集方面具有明显的竞争优势,而香港港对高端商品的吸引力更强;货运结构逐渐向中高端商品过渡对于提高港口收益具有促进作用,其中深圳港应通过降低商品库存成本而降低经营风险;深中通道和港珠澳大桥通车有效缩短腹地运输时间,对于绝大部分商品价格和库存成本组合都将提高粤港澳大湾区港口的总收益。     

南沙珊瑚礁区波纹钩鳞鲀(Balistapus undulatus)食性和营养级分析

波纹钩鳞鲀(Balistapus undulatus)是一种在热带珊瑚礁海域中广泛分布的杂食性鱼类, 研究其自然环境中的食物组成有助于了解其食物来源及其在生态系统中的功能地位。但目前的认识仅限于他们是珊瑚礁区的海胆捕食者, 对其准确的食物组成和生态功能定位尚不清晰。本研究于2017年夏季在南沙珊瑚礁区采集了波纹钩鳞鲀样品, 通过特异性引物扩增波纹钩鳞鲀消化道中的线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因, 以高通量测序技术分析了其现场食物组成, 并测定了碳、氮稳定同位素以分析其营养级。研究结果显示, 波纹钩鳞鲀摄食的食物种类有13种, 分属于节肢动物门(Arthropoda)、脊索动物门(Chordata)和环节动物门(Annelida)。其中最主要的食物来源是节肢动物门扇蟹科的蟹类, 如滑面蟹(Etisus sp.)、Luniella pubescens、皱纹花瓣蟹(Liomera rugata)等, 分别占61.8%、6.7%和1.8%。鱼类也有一定的贡献, 占总食物序列的23.5%。同位素结果显示其营养级为3.71±0.07, 与分子检测的结果相符。研究结果表明波纹钩鳞鲀的主要食物是小型甲壳类动物以及植食性鹦嘴鱼, 这拓展了以往对波纹钩鳞鲀所扮演生态角色的认识。     

FY-3D卫星MWHS-2辐射率资料直接同化对台风“米娜”预报的影响*

文章基于天气研究和预报(weather research and forecasting, WRF)模式中的FY-3D卫星微波湿度计Ⅱ(micro-wave humidity sounder 2, MWHS-2)辐射率资料的直接同化模块, 采用三维变分(three dimensional variation, 3DVar)方法在晴空条件下同化MWHS-2辐射率资料, 考察MWHS-2辐射率资料同化对台风“米娜”(2019)预报的影响。文中设计了4组试验, 第一组试验不同化任何资料, 第二组试验同化了单独的全球通信系统(global telecommunications system, GTS)常规资料, 第三组试验联合同化了GTS常规资料和MWHS-2辐射率资料, 第四组试验将MWHS-2辐射率资料换成先进技术微波探测计(advanced technology microwave sounder, ATMS)辐射率资料同化。研究结果表明: 偏差订正后各通道观测和背景场差值的均值趋于0, 同化后分析场相对观测的标准差与均方根误差较背景场显著减小, 同化过程是有效的。与仅同化GTS常规资料和同化ATMS资料的试验相比, 同化晴空MWHS-2辐射率资料后的增量场在台风中心附近有负的高度增量和正的温度增量, 从动力与热力上有助于台风的维持。在确定性预报最后的12h, 同化晴空MWHS-2辐射率资料的试验能够改进500hPa环流形势的模拟, 加强西南方向引导气流的强度, 从而最终减小台风路径预报的误差。     

Extraction and spatiotemporal evolution analysis of tidal flats in the Bohai Rim during 1984-2019 based on remote sensing

Tidal flats, a precious resource that provides ecological services and land space for coastal zones, are facing threats from human activities and climate change. In this study, a robust decision tree for tidal flat extraction was developed to analyse spatiotemporal variations in the Bohai Rim region during 1984-2019 based on 9539 Landsat TM/OLI surface reflection images and the Google Earth Engine (GEE) cloud platform. The area of tidal flats significantly fluctuated downwards from 3551.22 to 1712.36 km² in the Bohai Rim region during 1984-2019, and 51.31% of tidal flats were distributed near the Yellow River Delta and Liaohe River Delta during 2017-2019. There occurred a drastic spatial transition of tidal flats with coastline migration towards the ocean. Low-stability tidal flats were mainly distributed in reclamation regions, deltas, and bays near the estuary during 1984-2019. The main factors of tidal flat evolution in the Bohai Rim region included the direct impact of land cover changes in reclamation regions, the continuous impact of a weakening sediment supply, and the potential impact of a deteriorating sediment storage capability. The extraction process and maps herein could provide a reference for the sustainable development and conservation of coastal resources.     

The Influence of Plantation on Soil Carbon and Nutrients: Focusing on Tibetan Artificial Forests

As terrestrial ecosystem carbon (C) sinks, plantation ecosystems play essential roles in species diversity protection, resource supply and climate change. Artificial afforestation is of great important in improving the ecological condition, economic development and production in Tibet. Forests can improve soil property changes, yet the understanding of how plantations influence soil C and nutrient conditions in Tibet is still insufficient. This review combines with previous studies to explore the characteristics of soil nutrients, involving nitrogen (N) and phosphorus (P) on Tibetan poplar plantations. Generally, plantations have better abilities in improving the soil C and N cycles, and enhancing the soil stability. In this review, we further analyze the factors, including the modality of land-use, afforested period, tree species, climate factors and soil properties, which may affect the soil C and nutrients. (1) The patterns of land-use affect the accumulation of soil organic matter, thus influence the accumulation of soil C and nutrients; (2) Soil C and N increase with the years of artificial forests, while soil P is on the contrary; (3) The effects of different tree species on soil C and nutrients vary widely; (4) In terms of climate, the C sink of Tibetan plantation soil is most likely to be affected by precipitation, while the nutrient is more likely to be influenced by temperature; (5) Among soil properties, the most related factor to C is soil texture. Furthermore, our review pointed out that future research on soil ecological functions should be focused on soil microbes on Tibet plantation. At the end, we concluded three major challenges for the future research. Therefore, this review contributes to a better understand the effects of plantation on soil C and nutrients on the Tibetan Plateau.     

基于卷积神经网络的ICESat-2光子点云去噪分类

ICESat-2（Ice, Cloud, and land Elevation Satellite-2）是美国NASA（National Aeronautics and Space Administration）在2018年发射的激光测高卫星,其上搭载的激光测高系统ATLAS（Advanced Topographic Laser Altimeter System）采用微脉冲多波束光子计数激光雷达系统,因其低能耗、高探测灵敏度、高重复频率的特性极大改善了沿轨采样密度,但也使获取的数据中包含大量的噪声,如何有效实现光子点云去噪分类成为后续应用的关键,也是当前研究的热点和难点,为此本文提出一种基于卷积神经网络的光子点云去噪和分类算法。首先将光子点云按照沿轨和高程方向划分格网,去除明显的噪声光子,并将每个粗信号光子点栅格化为影像;然后基于少量样本构建的卷积神经网络分类模型实现光子点云精去噪和分类;最后利用机载激光雷达数据进行验证,并与ATL08产品的去噪分类结果进行对比。结果表明,对于裸地和森林区域,卷积神经网络算法均能有效去除噪声光子,特别对于森林区域,可同时实现去噪和分类;其中,裸地区域地表计算的R²和RMSE分别为1.0和0.72 m,森林区域地表和树冠计算的R²分别为1.0和0.70, RMSE分别为1.11 m和4.99 m。本文利用深度学习算法实现光子点云去噪分类,在裸地和森林区域均取得了较好的结果,为后续光子点云数据处理提供了参考。     

基于景观格局指数的基本农田保护区集中连片性研究——以济南市历城区为例

耕地与基本农田保护是我国土地管理、土地利用规划的重要内容。新一轮土地利用总体规划修编及数据库建设中要求基本农田调整布局更加集中连片。该文应用景观格局指数,研究了济南市历城区基本农田保护区规划调整中集中连片性的变化。结果表明,除保护区图斑面积标准差外,图斑平均面积、图斑面积变异系数、图斑边界密度、最大斑块指数、平均欧式邻近距离5项指标均表明,规划调整后基本农田保护区布局上更加集中连片。为基本农田保护区布局的集中连片性判断提供了简便易行的方法。     

潍坊市土地整治规划环境影响评价

从选取评价土地整治规划环境影响评价指标体系的原则入手,构建了2大方面、6大因素共计17项具体指标量化环境影响,通过综合分值来确定土地整治规划的实施对环境的具体影响,为整治规划的实施以及环境的保护提供参考。