Preservation of enzymic activity in marine plankton by low-temperature freezing

The measurement of enzymic activity in plankton communities is useful in the study of marine ecosystems. Such measurements can lead to a clearer understanding of the biological transformations in plankton communities at a particular time. However, the assays are somewhat time-consuming. To facilitate analysis of large numbers of samples, we have developed a method of quick-freezing of whole cells and of cell-free extracts of the diatom, Skeletonema costatum, followed by storage at ?60°C for up to one week. No loss in either the electron transport system (ETS) or the glutamate dehydrogenase (GDH) activity occurs. Similar conditions of storage can be used for the preservation of ETS activity in the marine copepod Calanus pacificus. Also, no measurable loss in either the GDH or the ETS activity is detectable after the quick-frozen whole cells of S. costatum have been kept frozen for over a year.     

Solubility of hydrous ferric oxide and iron speciation in seawater

Iron solubility equilibria were investigated in seawater at 36.22‰ salinity and 25°C using several filtration and dialysis techniques. In simple filtration experiments with 0.05 μm filters and Millipore ultra-filters, ferric chlorides fluorides, sulfates, and FeOH²⁺ species were found to be insignificant relative to Fe(OH)₂⁺ at p[H⁺] = ?log [H⁺] greater than 6.0. Hydrous ferric oxide freshly precipitated from seawater yielded a solubility product of ${}^1\text{K}{}_{\mathrm{<mtext>so</mtext>}}\text{=}\text{[Fe}{}^{\mathrm{3+}}\text{][H}{}^{\mathrm{+}}\text{]}{}^{\mathrm{?3}}\text{= 4.7 · 10}{}^5$. Solubility studies based on the rates of dialysis of various seawater solutions and on the filtration of acidified seawater solutions indicated the existence of the Fe(OH)₃⁰ species. The formation constant for this species can be calculated as ${}^1\text{β}{}_3\text{=}\text{[Fe(OH)}{}_3{}^0\text{] [H}{}^{\mathrm{+}}\text{]}{}^3\text{/[Fe}{}^{\mathrm{3+}}\text{] = 2.4 · 10}{}^{\mathrm{?14}}$. The Fe(OH)₄^? species is present at concentrations which are negligible compared to Fe(OH)₂⁺ and Fe(OH)₃⁰ in the normal pH range of seawater. However, there is at least one other significant ferric complex in seawater above p[H⁺] = 8.0 (possibly with bicarbonate, carbonate, or borate ions) in addition to the Fe(OH)₂⁺ and Fe(OH)₃⁰ species.     

Concentration–discharge relationships in the coal mined region of the New River basin and Indian Fork sub‐basin,Tennessee, USA

For many basins, identifying changes to water quality over time and understanding current hydrologic processes are hindered by fragmented and discontinuous water‐quality and hydrology data. In the coal mined region of the New River basin and Indian Fork sub‐basin, muted and pronounced changes, respectively, to concentration–discharge (C–Q) relationships were identified using linear regression on log‐transformed historical (1970s–1980s) and recent (2000s) water‐quality and streamflow data. Changes to C–Q relationships were related to coal mining histories and shifts in land use. Hysteresis plots of individual storms from 2007 (New River) and the fall of 2009 (Indian Fork) were used to understand current hydrologic processes in the basins. In the New River, storm magnitude was found to be closely related to the reversal of loop rotation in hysteresis plots; a peak‐flow threshold of 25 cubic meters per second (m³/s) segregates hysteresis patterns into clockwise and counterclockwise rotational groups. Small storms with peak flow less than 25 m³/s often resulted in dilution of constituent concentrations in headwater tributaries like Indian Fork and concentration of constituents downstream in the mainstem of the New River. Conceptual two or three component mixing models for the basins were used to infer the influence of water derived from spoil material on water quality. Copyright © 2012 John Wiley & Sons, Ltd.     

The relation between geomagnetic pulsations and an increase in the fluxes of geosynchronous relativistic electrons during geomagnetic storms

The dynamics of the Pc5 and Pi1 pulsation characteristics and relativistic electron fluxes at geostationary orbit were comparatively analyzed for three nine-day intervals, including quiet periods and periods of geomagnetic storms. It was shown that relativistic electron fluxes increase considerably when the power of global Pc5 pulsations and the index of midlatitude irregular Pi1 pulsations increase simultaneously. The correlation between the characteristics of Pi1 and Pc5 geomagnetic pulsations and the level of the relativistic electron flux at geostationary orbit during the magnetic storm recovery phase were studied. It was shown that the correlation coefficient of the relativistic electron maximal fluxes during the magnetic storm recovery phase with the parameter of midlatitude Pi1 pulsations is slightly higher than such a correlation coefficient with the solar wind velocity.     

Yield–reliability analysis for rural domestic water supply from combined rainwater harvesting and run-of-river abstraction

Abstract

In many rural areas, reticulated water supply from large water resources schemes does not exist and many households obtain water from groundwater (GW), rainwater harvesting (RWH), run-of-river (ROR) flow, or combinations of these. While comprehensive yield–reliability analysis for large water resource systems is the norm, typical rural water supply analysis unrealistically aggregates data into monthly or annual time steps and does not incorporate reliability. A daily time-step simulation of household supply and frequency analysis of the number of days supplied in each year is used herein to: (a) demonstrate a realistic approach of yield–reliability analysis for RWH, ROR and combined RWH and ROR supply; and (b) show how combined utilization of the two can lead to improved supply. Integration of RWH and ROR supply has been found to improve yield and reliability substantially. The limitations of using the simpler mass curve analysis and the effect of applying a monthly time step are demonstrated.

Citation Ndiritu, J., Odiyo, J. O., Makungo, R., Ntuli, C. & Mwaka, B. (2011) Yield–reliability analysis for rural domestic water supply from combined rainwater harvesting and run-of-river abstraction. Hydrol. Sci. J. 56(2), 238–248.     

A benchmark comparison of numerical methods for infinite Prandtl number thermal convection in two-dimensional Cartesian geometry

Abstract

A comparison is made between seven different numerical methods for calculating two-dimensional thermal convection in an infinite Prandtl number fluid. Among the seven methods are finite difference and finite element techniques that have been used to model thermal convection in the Earth's mantle. We evaluate the performance of each method using a suite of four benchmark problems, ranging from steady-state convection to intrinsically time-dependent convection with recurring thermal boundary layer instabilities. These results can be used to determine the accuracy of other computational methods, and to assist in the development of new ones.     

Predicting copper toxicity in estuarine and marine waters using the Biotic Ligand Model

The Biotic Ligand Model (BLM) has proven efficient in predicting the toxicity of a variety of metals to freshwater organisms. Consequently, the US EPA has proposed its use for calculating freshwater copper criteria. This study evaluates the BLM for use in estuarine and marine waters. Studies were conducted using the bivalve, Mytilus sp. and 48-h embryo-larval development chronic estimator test methods. These are the most sensitive taxa and test in the US EPA saltwater copper criteria database. Samples from five locations around the USA were tested. There is a strong relationship between measured and BLM predicted copper EC50s (log transformed data, r² = 0.76, p < 0.001, n = 44). The BLM predicted within a factor ±2 of measured EC50s in 41 of 44 cases. However, the BLM tends to predict lower EC50s when measured EC50s are approximately 10 μg Cu/L. This may be due to limitations of the metal-dissolved organic matter interaction model.     

ON THE CHARACTERISTICS OF NON-LINEAR SOIL RESPONSE AND DYNAMIC SOIL PROPERTIES USING VERTICAL ARRAY DATA IN JAPAN

Non-linear response of the soil is investigated by comparing the spectral ratios (uphole/downhole) using weak and strong motions. Data from seven vertical arrays in Japan are analysed in this study. The frequency-dependent transfer function of soil is calculated as a ratio of the spectrum at uphole to the spectrum at downhole, considering the horizontal component of shear wave. In spectral ratio analysis auto- and cross-spectra are employed. The reduction in the predominant frequency of the transfer function with increases in excitation level reflects the non-linear response of the soil. Results of analysis demonstrate a significant non-linear ground response at six sites with surface PGA exceeding 90 gal. However, the results of one site show the linear response up to 130 gal surface PGA. Furthermore, the in situ strain-dependent soil behaviour is examined through the shear modulus – shear strain relationship. When compared, the actual and laboratory results of the shear strain – shear modulus relationship are in agreement. Additionally, a good consistency between the tendency of reduction in shear modulus ratio with shear strain increases, and reduction of predominant frequency with ground motion increases, confirms the significance of non-linearity in site effects study.