Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

Electric Discharges in the Protoplanetary Nebula as a Source of Impulse Magnetic Fields to Promote Dust Aggregation

Lightning discharge generated in the protoplanetary nebula is viewed as a temporally isolated surge in the flow of electrically charged particles, similar to that of terrestrial lightning. If the current is intense enough, a powerful circular impulse magnetic field is generated around the instantaneous virtual electric conductor. Such magnetic field is capable of magnetizing dust grains containing ferromagnetic components present in its vicinity to their saturation levels. As a result, dust grains attract one another, forming the aggregates. This magnetically driven attraction suggests an important process possibly operational at an early stage of the planetary accretion. Based on both a classical model for electric conductor, and the theory of Lienard–Wiechert electromagnetic potentials, our calculations show that the magnetic impulse due to a discharge channel of a few cm in diameter transferring a charge of about 10⁴ electrons reaches as high as 10 T. At these magnetic fields, the ferromagnetic dust grains, and possibly the already-formed larger aggregates as well, are easily magnetized to the saturation levels, producing compact clusters exhibiting permanent magnetic moments.     

The Scattered Disk Population and the Oort Cloud

The trans-Neptunian belt has been subject to a strong depletion that has reduced its primordial population by a factor of one hundred over the solar system's age. One by-product of such a depletion process is the existence of a scattered disk population in transit from the belt to other places, such as the Jupiter zone, the Oort cloud or interstellar space. We have integrated the orbits of the scattered disk objects (SDOs) so far discovered by 2500 Myr to study their dynamical time scales and the probability of falling in each of the end states mentioned above, paying special attention to their contribution to the Oort cloud. We found that their dynamical half-time is close to 2.5 Gyr and that about one third of the SDOs end up in the Oort cloud.     

Colour Properties and Trends in Trans-Neptunian Objects

One of the most puzzling features of the Kuiper Belt, which has been confirmedby numerous surveys, is the optical colour diversity that seems to prevail among the observed TNOs. TNOs and Centaurs have surfaces showing dramaticallydifferent colours and spectral reflectances, from neutral to very red. With therelatively few visible-NIR colour datasets available, the colour diversity seemsalso to extend to the near infrared wavelengths. Relevant statistical analyses havebeen performed and several studies have pointed out strong correlations betweenoptical colours and some orbital parameters (i, e, q) for the Classical KuiperBelt objects. On the other hand, no clear trend is obvious for Plutinos, Scatteredobjects or Centaurs. Another important result is the absence of correlation of colourswith size or heliocentric distance for any of the populations of outer Solar Systemobjects. The strong colour anisotropy found is important because it is a diagnosticof some physical effects processing the surfaces of TNOs and/or some possiblecomposition diversity. In this paper, we will review the current knowledge of thecolour properties of TNOs, describe the observed colour distribution within theEdgeworth–Kuiper belt, and address the problem of their possible origin.     

Structural Evolution of the Eastern Margin of Eurasia in Late Mesozoic and Cenozoic

This paper features the structural evolution of the eastern margin of Eurasia in Late Mesozoic and Cenozoic.It is characterized by three stages of development: the riftogenic stage (Jurassic-Early Cretaceous), the platform stage (Late Cretaceous) and the neotectonic one (Paleogene-Quarternary). The boundaries between these stages are distinctly fixed by the geological time limits of planetary range. It is demonstrated that the riftogenic and neotectonic stages were characterized by a high degree of geodynamic activity, and the platform one by a decrease in contrast of tectonic movements. The main river net was formed in the Early Cretaceous and in the Neogene. It experienced a serious reconstruction accompanied by the formation of the Amur River valley being similar to the modem one.