Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Improved Resolution of Ambient Flow through Fractured Rock with Temperature Logs

In contaminant hydrogeology, investigations at fractured rock sites are typically undertaken to improve understanding of the fracture networks and associated groundwater flow that govern past and/or future contaminant transport. Conventional hydrogeologic, geophysical, and hydrophysical techniques used to develop a conceptual model are often implemented in open boreholes under conditions of cross-connected flow. A new approach using high-resolution temperature (±0.001°C) profiles measured within static water columns of boreholes sealed using continuous, water-inflated, flexible liners (FLUTe™) identifies hydraulically active fractures under ambient (natural) groundwater flow conditions. The value of this approach is assessed by comparisons of temperature profiles from holes (100 to 200 m deep) with and without liners at four contaminated sites with distinctly different hydrogeologic conditions. The results from the lined holes consistently show many more hydraulically active fractures than the open-hole profiles, in which the influence of vertical flow through the borehole between a few fractures masks important intermediary flow zones. Temperature measurements in temporarily sealed boreholes not only improve the sensitivity and accuracy of identifying hydraulically active fractures under ambient conditions but also offer new insights regarding previously unresolvable flow distributions in fractured rock systems, while leaving the borehole available for other forms of testing and monitoring device installation.     

Principal Components Analysis of Rainfall and Associated Synoptic Models of the Southwest Monsoon over India

Summary  The summer monsoon circulation shows various spatial and temporal oscillations and often a combination of systems produces an integrated effect. In this study phases of the southwest (SW) monsoon have been identified in an objective manner with the help of T-mode principal component analysis (PCA) of weekly rainfall anomalies. Mean composite charts have been prepared utilising all available upper air data (1977–1986) for each category of the SW monsoon epochs identified by the PCA. These sets of charts have been constructed for both the strong and weak phases associated with the first four significant principal components (PCs). A well defined east-west oriented trough system, extending from about 28° N Latitude/65° E Longitude to 20° N Latitude/90° E Longitude, in the lower levels, has been the main feature associated with the strong phase of the monsoon corresponding to PC I. The trough in the lower levels is more marked in the eastern half compared to the western half in both the sets of charts associated with strong phases of the monsoon related to the PC II and PC III. With PC II, the position of the troughs in the lower levels is further north of its location in PC III. The east-west trough system associated with the strong phase of PC IV has a large southward tilt with height. The charts corresponding to the weak phases of these PCs have synoptic features, such as the position of the trough close to the foothills of the Himalayas, and the shifting of middle and upper tropospheric anticyclones to the south. The study suggests an objective method of interpretation of principal components by utilising synoptic data. In addition, synoptic models and data sets corresponding to different phases of the monsoon can also be prepared in an objective manner by such PCA. Received July 18, 1997 Revised April 30, 1999     

The interpretation of body trace metal concentrations in neogastropods from Hong Kong.

Three species of neogastropods, i.e., Thais clavigera, Thais luteostoma and Morula musiva, were collected from 12 sites in the coastal waters of Hong Kong, including clean and known metal-enriched ones. Accordingly, body trace metal concentrations varied greatly. Copper and zinc body concentrations compared poorly with those of accepted biomonitors and presumed metal contamination profiles. Much is known about the feeding ecology of neogastropods on local shores and where direct observational data were not available, their diets could, nevertheless, be estimated reliably. Accumulated metal concentrations can be interpreted with regard to dietary history. T. luteostoma, for example, had body copper concentrations of 51.8 microg g(-1) on an exposed shore and a diet of mainly barnacles. In contrast, on a sheltered shore (but only some 250 m distant) where the diet consisted mainly of oysters, body copper concentrations were approximately 15 times greater at 833 microg g(-1). Similar results were obtained for T. clavigera. The results of this analysis suggest that neogastropods can play a significant role in trace metal trophic transfer studies, especially as their representatives are intertidally and subtidally ubiquitous and can be spatially separated into tropical specialists and temperate generalists.     

Models of travel demand with endogenous preference change and heterogeneous agents

In the literatures of regional science, urban economics, and urban development planning, a working assumption is that individuals respond to incentives and regulations, given their preferences. Models for planning and policy analyses are used to consider what might occur if the incentives or regulations were different. In these models, however, preferences are usually assumed to be given and stable, and agents are usually assumed to be homogeneous. This paper focuses on the implications of making preferences in models of policy implementation endogenously determined and time varying heterogeneous agents. We consider first the recent literature on intertemporal choice and preference change, which cuts across many disciplines, and more briefly the literature on norm-regarding behavior. We then elaborate a simple model of transportation demand—from a static to a dynamic orientation, from fixed and exogenously given preferences of strictly self-regarding agents to endogenously determined and policy-induced preferences of heterogeneous agents—and illustrate its characteristics with simple numerical examples.     

Mean crustal velocity: a critical parameter for interpreting crustal structure and crustal growth

Mean crustal velocity is a critical parameter for genesis of continental crystalline crust because it is a function of mean crustal composition and therefore may be used to resolve continental crustal growth in space and time. Although the best values of mean crustal velocity are determined from wide-angle reflection measurements, most studied here necessarily come from vertical averages in crustal refraction determinations. The mode of 158 values of mean crustal velocity is 6.3 km/s, a velocity which corresponds to a mean crustal composition of granodiorite to felsic quartz diorite; Archean crust may be slightly more mafic. Mean crustal velocities range from 5.8 to 7.0 km/s. The lowest values invariably are found in thermally disturbed rift zones and the highest values correspond to velocities in gabbro. Velocities in island arcs may be as low as 6.0 km/s but are typically 6.5–6.9 km/s which corresponds to andesitic composition; estimates of island arc composition are andesitic. If values of mean crustal velocity are not biased, this observation suggests that continental crust did not grow simply by addition of island arc material. Possibilities are that crust formed from fusion of island arcs and was later changed to more felsic composition by addition of material from the mantle or that the late Archean episode of major crustal growth did not involve processes similar to younger island arcs. Some crustal blocks might be changed in composition and thickness by such processes as underplating, interthrusting, necking and sub-crustal erosion. Specially designed experiments are suggested to determine this parameter so critical for understanding genesis of continental crust.     

Guided wave propagation in laterally varying media - II. Lg-waves in north-western Europe

Summary. Lg -wave observations at NORSAR from a sequence of explosions straddling the Central Graben in the North Sea, show that the phase is almost extinguished by passing through this structure. For models of the graben structure, based on seismic refraction experiments, with crustal thinning beneath a zone of thickened sediments, numerical modelling of Lg propagation shows very poor transmission. The inclusion of attenuation simulating the effects of scattering from faults and other complex structures further diminishes the transmittivity. The theoretical effect of a crustal pinch is to redistribute S energy over a wide range of groups velocities, a pattern that fits well with the observations.
Three components recordings at NORSAR of explosive charges fired in water covering a wide range of azimuths, show significant transverse components to the seismograms. The transverse energy builds up with travel time and for Lg is of the same order as the vertical component. These results are consistent with the theoretical prediction of progressive net transfer of energy from Rayleigh to Love modes as the wavetrain propagates through a three-dimensionally heterogeneous medium.     

Formalization of assessing radioactive waste burial site seismic regime parameters from seismological and geological data

A model is proposed that shows the relation of the block structure of the crust and earthquake sources (Sadovskii, 1979; Rodionov, 1979, 1984, 1994; Bugaev, 1999, 2011, 2014, 2015). The model can formalize how to assess the prediction of seismic regime parameters depending on the elastic limit and conditions and rate of deformation of the Earth’s crust. The spent nuclear fuel repository site in Olkiluoto (Finland) and a site in the area of the Krasnoyarsk Mining and Chemical Combine are considered as examples. It is demonstrated that the parameters of the prediction graphs limit the location of the points of magnitude repeatability graphs calculated for a site based on samples of earthquakes in the area according to different authors. This makes it possible to recommend predictive assessment of seismic regime parameters for stability monitoring of the seismic regime and safety analysis of a geological environment’s insulation properties for waste sites from the results of seismological monitoring and high-precision observations of modern movements of the Earth’s crust.