Hydrogen isotope fractionation in lipids of the methane-oxidizing bacterium Methylococcus capsulatus

Hydrogen isotopic compositions of individual lipids from Methylococcus capsulatus, an aerobic, methane-oxidizing bacterium, were analyzed by hydrogen isotope-ratio-monitoring gas chromatography-mass spectrometry (GC-MS). The purposes of the study were to measure isotopic fractionation factors between methane, water, and lipids and to examine the biochemical processes that determine the hydrogen isotopic composition of lipids. M. capsulatus was grown in six replicate cultures in which the δD values of methane and water were varied independently. Measurement of concomitant changes in δD values of lipids allowed estimation of the proportion of hydrogen derived from each source and the isotopic fractionation associated with the utilization of each source.All lipids examined, including fatty acids, sterols, and hopanols, derived 31.4 ± 1.7% of their hydrogen from methane. This was apparently true whether the cultures were harvested during exponential or stationary phase. Examination of the relevant biochemical pathways indicates that no hydrogen is transferred directly (with C-H bonds intact) from methane to lipids. Accordingly, we hypothesize that all methane H is oxidized to H₂O, which then serves as the H source for all biosynthesis, and that a balance between diffusion of oxygen and water across cell membranes controls the concentration of methane-derived H₂O at 31%. Values for α_l/w, the isotopic fractionation between lipids and water, were 0.95 for fatty acids and 0.85 for isoprenoid lipids. These fractionations are significantly smaller than those measured in higher plants and algae. Values for α_l/m, the isotopic fractionation between lipids and methane, were 0.94 for fatty acids and 0.79 for isoprenoid lipids. Based on these results, we predict that methanotrophs living in seawater and consuming methane with typical δD values will produce fatty acids with δD between −50 and −170‰, and sterols and hopanols with δD between −150 and −270‰.     

Two magma series and associated ore deposit types in the Permian Emeishan large igneous province, SW China

The Late Middle Permian ( 260 Ma) Emeishan large igneous province in SW China contains two magmatic series, one comprising high-Ti basalts and Fe-rich gabbroic and syenitic intrusions, the other low-Ti basalts and mafic–ultramafic intrusions. The Fe-rich gabbros are spatially and temporally associated with syenites. Each series is associated with a distinctive type of mineralization, the first with giant Fe–Ti–V oxide ore deposits such as Panzhihua and Baima, the second with Ni–Cu–(PGE) sulfide deposits such as Jinbaoshan, Limahe and Zhubu. New SHRIMP zircon U–Pb isotopic data yielded 263 ± 3 Ma for the Limahe intrusion, 261 ± 2 Ma for the Zhubu intrusion and 262 ± 2 Ma for a syenitic intrusion. These new age dates, together with previously reported SHRIMP zircon U–Pb ages, suggest that all these intrusions are contemporaneous with the Emeishan flood basalts and formed during a major igneous event at ca. 260 Ma.The oxide-bearing intrusions have higher Al₂O₃, FeO (as total iron) and total alkalis (Na₂O + K₂O) but lower MgO than the sulfide-bearing intrusions. All intrusions are variably enriched in LREE relative to HREE. The oxide-bearing intrusions display positive Nb- and Ti-anomalies and in certain cases negative Zr–Hf anomalies, whereas the sulfide-bearing intrusions have obvious negative Nb- and Ti-anomalies, a feature of crustal contamination. Individual intrusions have relatively small ranges of Nd(t) values. All the intrusions, however, have Nd(t) values ranging from − 3.9 to + 4.6, and initial ⁸⁷Sr/⁸⁶Sr ratios from 0.7039 to 0.7105. The syenites have very low MgO (< 2 wt.%) but highly variable Fe₂O₃ (2.5 to 13 wt.%) with initial ⁸⁷Sr/⁸⁶Sr ratios ranging from 0.7039 to 0.7089. Magmas from both series could have derived by melting of a heterogeneous mantle plume: the high-Ti series from a Fe-rich, more fertile source and the low-Ti series from a Fe-poor, more refractory source. In addition, the low-Ti series underwent significant crustal contamination. The two magma series evolved along different paths that led to distinct mineralization styles.     

Turbulent Flow Properties Around a Staggered Wind Farm

The fundamental properties of turbulent flow around a perfectly staggered wind farm are investigated in a wind tunnel. The wind farm consisted of a series of 10 rows by 2–3 columns of miniature wind turbines spaced 5 and 4 rotor diameters in the streamwise and spanwise directions respectively. It was placed in a boundary-layer flow developed over a smooth surface under thermally neutral conditions. Cross-wire anemometry was used to obtain high resolution measurements of streamwise and vertical velocity components at various locations within and above the wind farm. The results show that the staggered configuration is more efficient in terms of momentum transfer from the background flow to the turbines compared to the case of an aligned wind turbine array under similar turbine separations in the streamwise and spanwise directions. This leads to improved power output of the overall wind farm. A simplified analysis suggests that the difference in power output between the two configurations is on the order of 10%. The maximum levels of turbulence intensity in the staggered wind farm were found to be very similar to that observed in the wake of a single wind turbine, differing substantially with that observed in an aligned configuration with similar spacing. The dramatic changes in momentum and turbulence characteristics in the two configurations show the importance of turbine layout in engineering design. Lateral homogenization of the turbulence statistics above the wind farm allows for the development of simple parametrizations for the adjustment of flow properties, similar to the case of a surface roughness transition. The development of an internal boundary layer was observed at the upper edge of the wind farm within which the flow statistics are affected by the superposition of the ambient flow and the flow disturbance induced by the wind turbines. The adjustment of the flow in this layer is much slower in the staggered situation (with respect to its aligned counterpart), implying a change in the momentum/power available at turbine locations. Additionally, power spectra of the streamwise and vertical velocity components indicate that the signature of each turbine-tip vortex structure persists to locations deep within the wind farm.     

Suevite breccia from the Ries crater,Germany: Origin,cooling history and devitrification of impact glasses

Abstract— The distribution and petrography of surficial suevite breccias of the Ries impact crater in Southern Germany are reviewed, and the morphology, petrography and chemical composition of impact glasses in suevite breccias and their postdepositional devitrification is synthesized. Origin and thermal history of suevite breccia and suevite glasses are inferred from these data and from recent results of cooling and crystallization experiments with suevite glass melts under controlled conditions. In a montmorillonitic groundmass, the suevite breccia contains pieces of glass, up to some decimeters in size, and crystalline rock clasts of all stages of shock metamorphism. The glass particles originated in impact melt of basement gneisses and cooled by adiabatic pressure release from ～80 GPa to atmospheric pressure during ejection from the crater. They were deposited on the ground together with the other suevite components at a temperature of ～750 °C. Fractured glass pieces in the breccia show that during deposition of the suevite the temperature was below the temperature at which undercooled melt transforms to rigid glass. The suevite cooled after deposition mainly by convection of heat by emanating gases and vapors. In chilled layers at the base and at the top of suevite deposits, the glasses are preserved in vitreous state. Between these zones, the glasses were devitrified, yet crystallization of pyroxene, plagioclase and magnetite took place below the glass-transformation temperature. Annealing experiments show that this unusual devitrification below the transformation temperature can be explained by the impact origin of suevite glasses. Due to rapid adiabatic cooling on decompression, the glasses were oversaturated with water and internally strained. Under these conditions, devitrification, especially the formation of plagioclase, was possible at temperatures below the transformation range. The origin from adiabatically cooled impact melt of deep-seated rocks distinguishes water-bearing suevite glasses from the Ries-derived, water-free moldavite tektites, which are interpreted as condensates of vaporized, surficial sediments (Engelhardt et al., 1987).     

Geophysical investigations of large landslides in the Carnic Region of southern Austria

The area under investigation for the past two decades is in the vicinity of the Gailtal lineament, which is the most dominant tectonic feature of the eastern Alps of southern Austria. An area of about 8 km² is in a state of constant instability, as documented by movement of road tracks of several centimetres per year. Geotechnical and surveying techniques have been used to measure these movements in the past but without solving the problem of the mechanism of these failure processes. Geophysical methods (seismic refraction, geoelectrics, and electromagnetics) were applied in order to determine the validity of one of the discussed movement models. In-situ velocity measurements were used to identify different lithologies beneath surficial talus deposits. The thickness of these talus deposits, of about 4–30 m, found by seismic refraction clearly demonstrates that huge ‘blocks’ (i.e. more or less undisturbed lithologic units) within the talus/debris are in close contact with the basement. This basement, which shows lower seismic velocities in different parts combined with low electric resistivities, is obviously strongly disturbed by different failure surfaces. The different gliding velocity of the blocks and the talus/debris deposits leads to a geological model in which huge rock blocks move slowly in relation to the disintegrating basement, whereas the talus/debris deposits move over the surface of these blocks at a higher velocity. The interpretation of these landslide studies is not a straightforward analysis. It is a complex problem with a complex solution, including all information from geotechnical, geophysical, and surveying investigations.     

The discrete and localized nature of the variable emission from active regions

Using data from the Extreme Ultraviolet (EUV) Spectroheliometer onSkylab, we study the empirical characteristics of the variable emission in active regions. These simultaneous multi-wavelength observations clearly confirm that active regions consist of a complex of loops at different temperatures. The variable emission from this complex has very well-defined properties that can be quantitatively summarized as follows: (1) It is localized predominantly around the footpoints where it occurs at discrete locations. (2) The strongest variability does not necessarily coincide with the most intense emission. (3) The fraction of the area of the footpoints,δn/N, that exhibits variable emission, varies by ±15% as a function of time, at any of the wavelengths measured. It also varies very little from footpoint to footpoint. (4) This fractional variation is temperature dependent with a maximum around 10⁵ K. (5) The ratio of the intensity of the variable to the average background emission, δI/Ī, also changes with temperature. In addition, we find that these distinctive characteristics persist even when flares occur within the active region.     

Modeling and Simulation of Supercavity with Inertial Force in the Horizontal Curvilinear Motion

To make a curvilinear motion in the horizontal plane is one of the most contents for realizing the maneuverability of the supercavitating vehicle. It is significant to achieve the controllability and maneuverability of the vehicle in three dimensions both theoretically and practically on research. Models of angle of attack, gravity and inertial force effects on the supercavity in the horizontal curvilinear motion are established, respectively. The supercavity is simulated based on these models in combination with Logvinovich model and the unsteady gas-leakage rate model at the given ventilation rate, and the effect of the inertial force on it is analyzed numerically. Results show that the maximum deviation of the center line of the cross section of supercavity towards the outward normal direction of its trajectory increases as the cavitation number or curvature radius decrease and always occur in the tail because of the increase of inertial effects along the axis of supercavity from the cavitator when other models and flow parameters are constant for the given trajectory curvature. For the variable curvature, the supercavity sheds due to its instability caused by the time-varying angle of attack. The deviation increases along the length of supercavity if the curvature remains the same sign.     

Rare earth element abundances in a thick,layered komatiite lava flow from Ontario,Canada

Rare earth element abundances have been measured in pyroxenitic (19.6% MgO) to gabbroic (7.7% MgO) rocks from the upper part of a thick, layered komatiite lava flow (Fred's Flow) in Munro Township, Ontario. This flow apparently erupted as a highly basic liquid which subsequently differentiated into layers of ultramafic cumulate rocks and a basaltic residual liquid. The analyzed rocks have compositions and spinifex or equigranular textures interpreted to indicate that they represent the complete range of liquids that were present during the differentiation of the lava.All the analyzed rocks are depleted in light REE, and also exhibit a slight depletion of Yb and Er relative to Gd and Dy. Chondrite-normalized Ce and Yb abundances range from 3.2 to 7.8 and 5.1 to 9.7 respectively. Proportions of fractionating minerals were estimated using a major element petrological mixing program and petrographic data. REE modeling based on these results indicates that the dominant process relating the samples is low-pressure fractional crystallization of olivine, followed at lower temperatures by clinopyroxene and plagioclase. Except for Eu, correspondence between observed and calculated REE abundances obviates any need to appeal to processes of major REE redistribution during diagenesis and low-grade metamorphism. Major differences in REE patterns of other ultramafic and mafic komatiitic lava flows [6,11], therefore, probably reflect different episodes of partial melting and/or differences in mantle source composition. The consistency of the REE in the layered flow, however, supports the concept that mafic komatiites can also be derived from ultrabasic parental magmas by low-pressure fractional crystallization. The light-REE-depleted patterns of these komatiites resemble those of modern MORB, suggesting that the mantle source of the komatiites had undergone a previous melting episode.     

Elemental and stable isotope variations of organic matter from a terrestrial sequence containing the Cretaceous/Tertiary boundary at York Canyon,New Mexico

Elemental and isotopic composition of organic matter from a terrestrial sequence including the palynological Cretaceous/Tertiary (K-T) boundary together with an Ir anomaly at York Canyon, New Mexico, record information about paleoclimatological and environmental conditions. Six layers of coal, carbonaceous shale and mudstone with high contents of organic material were selected for analysis. A Late Cretaceous coal bed 10 m below the K-T boundary and an Early Tertiary coal bed containing the K-T iridium anomaly at its base were sampled intensively. In the lower bed, the isotopic ratios¹³C/¹²C,¹⁵N/¹⁴N,andD/H and the elemental ratiosC/N andN/H, all varied sympathetically with one another over depth. In contrast, in the upper coal layer, only theD/H,C/N,andN/H ratios showed some coupling. Immediately above the K-T boundary, theδ¹³C values displayed a long-term shift of 1.8‰ to more negative values, while the hydrogen isotope ratios in these samples did not change significantly. We interpret the covariations in both coal layers as sympathetic responses of the isotopic and elemental ratios to climatic and environmental changes, as have been observed for younger sedimentary organic matter. The long-termδ¹³C shift during the early Tertiary is similar to the trend observed forδ¹³C values of marine carbonates. Our data thus support the proposal that the carbon cycle was perturbed globally by the effects of a drastically decreased marine bioproductivity along the K-T transition. The uncoupling of variations in the climatically sensitive isotopic and elemental ratios seen in the Early Tertiary coal bed provides evidence for major geochemical and environmental changes in the York Canyon area at the end of the Mesozoic. On the other hand, the constancy of δD values in the organic matter deposited at and above the K-T boundary gives no indication of significant changes in the hydrologic/climatic regimes as recorded in theD/H ratios at the site for several thousand years following the event which produced the high Ir concentrations. Our results provide constraints on models that have been advanced to explain that event and its consequences.