Genetic impact of the Prestige oil spill in wild populations of a poor dispersal marine snail from intertidal rocky shores

In November 2002, the sinking of the Prestige cargo ship produced an oil spill of 60,000 tons that affected many areas along the Galician coast (in the northwest of Spain). In a number of rocky shore sites, most organisms (particularly marine mollusks) were nearly extinct at a local scale. We tested whether the local bottleneck/extinction that occurred in affected localities caused any detectable reduction of the genetic diversity in the marine snail Littorina saxatilis, an ovoviviparous rocky shore model species characterized by a low dispersal ability, high population density, and wide distribution range. We compared the level of genetic variation and population differentiation between affected (polluted) and control sites located in seven geographical areas (three sites per area, one impacted and two controls, and two replicates per site) one and a half years after the spill. The analysis included molecular marker variation (microsatellite and AFLP loci) and quantitative trait genetic variation for shell variables in embryos extracted from pregnant females. Our results indicate that the affected populations did not show a significant overall reduction in genetic diversity when compared to the controls, suggesting that the species is highly resistant to losing genetic variability as a consequence of a local short-term pollution process in spite of its low dispersal ability and direct development. However, some genetic effects were detected in the polluted populations, particularly for quantitative shell traits and AFLPs, consistent with local adaptations resulting from the fuel contamination.     

Carbonaceous materials in size-segregated atmospheric aerosols from urban and coastal-rural areas at the Western European Coast

A high-volume cascade impactor, equipped with a PM10 inlet, was used to collect size-segregated aerosol samples during the summer of 2004 at two Portuguese locations: a coastal-rural area (Moitinhos) and an urban area (Oporto). Concentrations of airborne particulate matter (PM), total carbon (TC), organic carbon (OC), elemental carbon (EC), and water-soluble organic carbon (WSOC) were determined for the following particle size ranges: < 0.49, 0.49–0.95, 0.95–3.0, and 3.0–10 µm. The total PM mass concentrations at the urban and coastal-rural sites ranged from 22.8 to 79.6 μg m^− 3 and 19.9 to 28.2 μg m^− 3, respectively, and more than 56% of the total aerosol mass was found in the fractions below 3.0 μm. At both locations the highest concentrations of OC and EC were found in the submicrometer size range. The regional variability for the OC and EC concentrations, with the highest concentrations being found in the urban area, was related to the contribution of local primary sources (mostly traffic emissions). It was also verified an enrichment of the small size particles in WSOC, representing on average 37.3(± 12.4)% and 59.7(± 18.0)% of OC in the very fine aerosol at the coastal-rural and urban areas, respectively. The amount of secondary OC calculated by the minimum OC/EC ratio method indicates that secondary organic aerosol formation was important throughout the study at both sites. The obtained results suggest that long-range transport and favourable summer conditions for photochemical oxidation are key factors determining secondary OC formation in the coastal-rural and urban areas. The ultraviolet absorption properties of the chromophoric constituents of the WSOC fractions were also different among the different particle size ranges and also between the two sampling locations, thus suggesting the strong impact of the diverse emission sources into the composition of the size-segregated organic aerosol.     

Colony growth in the harvested octocoral Pseudopterogorgia acerosa in a Caribbean coral reef

Pseudopterogorgia acerosa is the tallest gorgonian octocoral found in the Caribbean. In the Cartagena area, Colombia, P. acerosa is commonly harvested as raw material for handicrafts, rendering important the study of its growth and population dynamics. Growth measurements of branches from multiple colonies at two different reefs during 1 year revealed that the main growth was due to rapid elongation of the main (‘mother’) branch and production of daughter branches from the same (or just a few) mother branch(es). Some small colonies doubled their original size in a year. The branches’ absolute growth rate was weakly correlated with size, with no significant relationships between branch size and percent growth. While the pinnate branching pattern in P. acerosa is similar to its congeners Pseudopterogorgia elisabethae and Pseudopterogorgia bipinnata, it produces taller colonies due to the faster growth rate in the primordial mother branch. This growth mode seems advantageous as it augments exposure to light and higher fluxes of suspended food in the benthic boundary layer. Post‐harvesting, clipped colonies exhibited clear signs of regeneration, even in cases where only the holdfast remained. Pseudopterogorgia acerosa seems to have similar regeneration capabilities respect to other Pseudopterogorgia species, where regenerating colonies may grow faster than intact ones.     

Frequency of tidal storm surge at New York harbor (New York) and Newark bay (New Jersey)

A joint probability analysis of storm tides is made for the Battery, New York (U.S.A.), and Newark Bay, New Jersey (U.S.A.). Storm tides are caused by either low-pressure coastal storms (northeasters) or hurricanes. The analysis uses existing gage data at the Battery to construct frequency/probability curves of peak surge elevations and tidal amplitudes. These are used in a computer program to combine standard storm surge stages and tidal stage curves to produce an artificial storm tide with given peak elevation and incremental frequency. These peak elevations and incremental frequencies are ranked to produce cumulative frequency curves for northeasters and hurricanes. A frequency curve is obtained for Newark Bay via correlation.     

Application of Non-Ionic Solid Sorbents (XAD Resins) for the Isolation and Fractionation of Water-Soluble Organic Compounds from Atmospheric Aerosols

A detailed procedure using non-ionic macropourous XAD-8 and XAD-4 resins is presented for the isolation and fractionation of aerosol water-soluble organic compounds (WSOC) from aerosol samples. The procedure entails adsorption of WSOC fraction onto XAD-8 and XAD-4 resins, desalting of the adsorbed organic material with ultra-pure water, elution of the retained organic matter with 40% MeOH solution and freeze-drying. Due to resin’s different properties and to certain hydrophobic/hydrophilic interactions between the resin polymers and the organic matter, two major fractions were obtained; namely the XAD-8 and the XAD-4 eluates. The XAD-8 eluate, which accounts for 55–60% of total aerosol WSOC, is represented by partially acidic compounds with significant hydrophobic moieties. The XAD-4 fraction holds few conjugated systems and a higher content of hydrophilic structures with low molecular size, and accounts for 9% of total WSOC. The isolated WSOC sub-fractions were nearly free from inorganic species, and successful recoveries of organic matter from the resins were accomplished. With this procedure the XAD-8 eluate yields a mixture representative of those WSOC that are highly conjugated compounds in atmospheric aerosols. It also allows a successful characterisation of the organic material by advanced analytical techniques without the interference of inorganic species present in the original sample of atmospheric particles.     

Pretunnel technology: applications and design methods

The Pretunnel technology allows the final or preliminary lining to be built ahead of a tunnel face. The paper shows how this technology has been used for mechanizing the full-face excavation of large tunnels in difficult ground conditions with minimum settlements. Analytical methods are presented for the analysis of the lining both in deep and shallow applications. Fields of applicability are established using results of parametric studies based on the methods proposed.     

Stratigraphy and allogenic controls of the western Portugal Cretaceous: an updated synthesis

The stratigraphy of the western Portugal on-shore Cretaceous record (western Iberian margin, Lusitanian Basin) is described, including formal units and a selection of informal units prevailing in the geological literature. This paper is a synthesis based on a review of previous works, but with an innovative emphasis on the interpretation of eustatic and tectonic controls. The sedimentary record is dominated by siliciclastics and comprises fluvial and deltaic coastal marine siliciclastic systems, as well as extensive deposits of shallow marine carbonate platforms, both open and rimmed. Several regional unconformities and transgressive/regressive cycles are identified and the allogenic controls interpreted, namely the geodynamic events along the boundaries of the Iberian plate. Above the Berriasian deposits belonging to the Upper Jurassic cycle, the five main unconformity-bounded units are: (1) upper Berriasian–lower Barremian, (2) upper Barremian–lower Aptian, (3) upper Aptian–uppermost Cenomanian, (4) mid lower Turonian–lower Campanian and (5) middle Campanian–Maastrichtian. These units show transgressive peaks in the lower Hauterivian, lower Aptian, base of the upper Cenomanian and mid lower Turonian. The general trend of the Lower Cretaceous reflects the transition from late rifting to passive margin, with the last break-up unconformity dated as late Aptian. The Lusitanian Basin achieved full infill by the Cenomanian, when a large carbonate platform extended far inland. The later deposits were preserved only in the northern sector and the accompanying unconformities reflect transpressive intraplate stresses generated in boundaries of the plate with Africa and Eurasia. With very low accommodation being created throughout the Late Cretaceous, fluvial deposits were dominant, including a few marine levels related with eustatic rises in the early Turonian, the Coniacian, the early Campanian and the Maastrichtian.     

Performance of the veto detector incorporated into the ZEPLIN-III experiment

The ZEPLIN-III experiment is operating in its second phase at the Boulby Underground Laboratory in search of dark matter WIMPs. The major upgrades to the instrument over its first science run include lower background photomultiplier tubes and installation of a plastic scintillator veto system. Performance results from the veto detector using calibration and science data in its first six months of operation in coincidence with ZEPLIN-III are presented. With fully automated operation and calibration, the veto system has maintained high stability and achieves near unity live time relative to ZEPLIN-III. Calibrations with a neutron source demonstrate a rejection of 60% of neutron-induced nuclear recoils in ZEPLIN-III that might otherwise be misidentified as WIMPs. This tagging efficiency reduces the expected untagged nuclear recoil background from neutrons during science data taking to a very low rate of ?0.2 events per year in the WIMP acceptance region. Additionally, the veto detector provides rejection of 28% of γ-ray induced background events, allowing the sampling of the dominant source of background in ZEPLIN-III - multiple scatter γ-rays with rare topologies. Since WIMPs will not be tagged by the veto detector, and tags due to γ-rays and neutrons are separable, this population of multiple scatter events may be characterised without biasing the analysis of candidate WIMP signals in the data.     

Leaching and dilution of fertilizers in the Yucatan karstic aquifer

The application of agrochemicals in crops can cause pollution of aquifers, especially those with a shallow water table and covered by thin soils; such is the case of Yucatan, Mexico. Information about the requirements and application of fertilizers to major irrigation and rainfed crops as well as the volume of water consumed in growing were used to determine the potential amount of nitrate delivered to groundwater. The research reported in this paper was conducted in the metropolitan area of the city of Merida, in Yucatán, Mexico, where groundwater plays an important role since it is the main source of supply, due to the lack of surface water in the region. In addition, the vulnerability of this aquifer is threatened by the various activities taking place in the municipalities, which emphasize agriculture, located within the groundwater basin. This activity is analyzed as a source of contamination given the concentration of NO₃ ^? present in groundwater intended for human consumption. The results show that the contribution of farming that enriches the presence of nitrates in the aquifer is different for each municipality in the study area. For the ZMM, the average nutrient leaching is 44 % of the original mass applied; it could be diluted approximately 12 times, in the groundwater.