Species richness,diversity and density of understory vegetation along disturbance gradients in the Himalayan conifer forest

We investigated whether species richness, diversity and density of understory herbaceous plants differed along logging(gap) and grazing(primarily by cattle) disturbance gradients, and sought to identify drivers of richness, diversity and density of understory vegetation of logged sites. A factorial experiment was conducted in the mixed conifer forest of Gidakom in Western Bhutan. Levels of the logging treatment included small(0.15 – 0.24 ha), medium(0.25 – 0.35 ha) and large(0.36 – 1.31 ha) gaps. The grazing treatment included grazed(primarily by cattle) and ungrazed(where herbivores were excluded by a fence) plots nested within each gap. Data were collected from 12 gaps(4 replicates at each level of logging) using the point intercept method. Shannon Weaver Diversity and Margalef's indices were used to estimate species diversity and describe species richness, respectively. Soil samples were analyzed for pH and nutrients. The interaction effect of logging and grazing was significant(p≤0.001) only on species diversity. Relative to ungrazed areas, species diversity was significantly higher(0.01≤p≤0.05) in medium grazed gaps. Under grazed conditions, soil P was negatively correlated with gap size and species diversity. While species diversity was positivelycorrelated(0.01≤p≤0.05) with soil N in grazed plots species richness was positively correlated(0.001≤p≤0.01) with soil N in ungrazed plots. Relative density of Yushania microphylla and Carex nubigena were higher under ungrazed conditions. Our study suggests that the combined effect of cattle grazing and logging results in higher species diversity of understory vegetation in medium and grazed gaps in mixed conifer forests of Bhutan,whereas increase or decrease in relative density of major species is determined primarily by the independent effects of grazing and logging. From management perspective, forest managers must refrain from creating large gaps to avoid loss of nutrients(mainly P and N), which may eventually affect tree regeneration. Managers intending to maintain understory vegetation diversity must consider the combined effects of grazing and logging, ensuring low to moderate grazing pressure.     

Longshore drift estimation using fluorescent tracers: New insights from an experiment at Comporta Beach, Portugal

A fluorescent sand-tracer experiment was performed at Comporta Beach (Portugal) with the aim of acquiring longshore sediment transport data on a reflective beach, the optimization of field and laboratory tracer procedures and the improvement of the conceptual model used to support tracer data interpretation.

The field experiment was performed on a mesotidal reflective beach face in low energetic conditions (significant wave height between 0.4 and 0.5 m). Two different colour tracers (orange and blue) were injected at low tide and sampled in the two subsequent low tides using a high resolution 3D grid extending 450 m alongshore and 30 m cross-shore. Marked sand was detected using an automatic digital image processing system developed in the scope of the present experiment.

Results for the two colour tracers show a remarkable coherence, with high recovery rates attesting data validity. Sand tracer displayed a high advection velocity, but with distinct vertical distribution patterns in the two tides: in the first tide there was a clear decrease in tracer advection velocity with depth while in the second tide, the tracer exhibited an almost uniform vertical velocity distribution. This differing behaviour suggests that, in the first tide, the tracer had not reached equilibrium within the transport system, pointing to a considerable time lag between injection and complete mixing. This issue has important implications for the interpretation of tracer data, indicating that short term tracer experiments tend to overestimate transport rates. In this work, therefore, longshore estimates were based on tracer results obtained during the second tide.

The estimated total longshore transport rate at Comporta Beach was 2 × 10^− 3 m³/s, more than four times larger than predicted using standard empirical longshore formulas. This discrepancy, which results from the unusually large active moving layer observed during the experiment, confirms the idea that most common longshore transport equations under-estimate total sediment transport in plunging/surging waves.     

Seasonal variations of water system distribution and flow patterns in the southern sea area of Hokkaido,Japan

Hydrographic data and composite current velocity data (ADCP and GEK) were used to examine the seasonal variations of upper-ocean flow in the southern sea area of Hokkaido, which includes the “off-Doto” and “Hidaka Bay” areas separated by Cape Erimo. During the heating season (April–September), the outflow of the Tsugaru Warm Current (TWC) from the Tsugaru Strait first extends north-eastward, and then one branch of TWC turns to the west along the shelf slope after it approaches the Hidaka Shelf. The main flow of TWC evolves continuously, extending eastward as far as the area off Cape Erimo. In the late cooling season (January–March), part of the Oyashio enters Hidaka Bay along the shallower part of the shelf slope through the area off Cape Erimo, replacing almost all of the TWC water, and hence the TWC devolves. It is suggested that the bottom-controlled barotropic flow of the Oyashio, which may be caused by the small density difference between the Oyashio and the TWC waters and the southward migration of main front of TWC, permits the Oyashio water to intrude along the Hidaka shelf slope.     

Spatial variability in growth and reproduction of the Pacific oyster Crassostrea gigas (Thunberg, 1793) along the west European coast

The Pacific oyster Crassostrea gigas was introduced in Europe for commercial purposes in the mid 1960s. It was initially thought that low winter temperatures would restrain this species' reproduction and settlement; however, its present distribution in areas where no introduction has taken place suggests that natural invasion and expansion has occurred. Along the European coast, wild populations of Pacific oysters are already found from northern Germany to southern Portugal. Whether C. gigas will continue to further expand through northern waters will depend on its physiological performance. In this study, the performance of wild oyster populations has been studied in terms of growth and reproduction at three stations: La Rochelle (France; 46°N), Yerseke (Oosterschelde estuary, The Netherlands, 51°N), and Texel (Wadden Sea estuary, The Netherlands, 53°N). The French population had the lowest somatic-shell mass ratio and an increase in maximum shell length, somatic and gonadal mass was observed from France to the Netherlands. In addition, mean oocyte diameter decreased significantly from south to north. The combination of increasing gonadal mass and decreasing oocyte volume suggests an increasing reproductive output in terms of egg numbers from France to The Netherlands. Differences in temperature between locations will at least be partly responsible for the observed patterns; however, other environmental factors (such as food availability, predation pressure, sediment type and/or seston concentration) cannot be excluded. Since smaller eggs (oocytes) are thought to have a longer development time, the environmental conditions along the Dutch coast may result in increased larval dispersal and possibly in further population expansion.     

The effect of the dominant polychaete Scolelepis squamata on nematode colonisation in sandy beach sediments: An experimental approach

The effect of an abundant sandy beach polychaete, Scolelepis squamata, on the colonisation of defaunated sediments by marine nematodes indicates that sandy beach fauna can be partially controlled by biological interactions within and across size groups. Experimental cores, equipped with windows allowing infaunal colonisation, were filled with defaunated sandy beach sediment containing two different treatments with and without S. squamata. These cores were inserted into microcosms filled with sediment with indigenous meiofauna collected from the field. The treatments were incubated in the laboratory at ambient temperature and salinity for 2, 7, 14 and 21 days, in order to follow the colonisation process of the defaunated sediments by the indigenous nematode fauna over time. Nematodes initially colonised both treatments, with abundances of up to 10% of the densities in the control; after 2 weeks, nematode densities in the cores without S. squamata surpassed the control densities. Nematode assemblages in both treatments were not species rich, and also differed in composition from the natural assemblages. The most successful colonising species, Enoplolaimus litoralis, was rare in the surrounding sediment, suggesting that colonisation was determined by species-specific characteristics such as body size, motility and feeding strategy. Initially the presence of macrofauna did not affect the nematode community composition, but after 2 weeks of the experiment, the presence of the polychaete seemed to facilitate the earlier establishment of non-opportunistic species.     

Deep-sea nematode assemblage has not recovered 26 years after experimental mining of polymetallic nodules (Clarion-Clipperton Fracture Zone, Tropical Eastern Pacific)

We investigated nematode assemblages inhabiting the 26-year-old track created by experimental deep-sea mining of polymetallic nodules, and two adjacent, undisturbed sites, one with nodules and one without nodules. The aim was to compare density, assemblage structure, and diversity indices in order to assess the process of recovery of the nematode assemblage inhabiting the disturbed site. This experimental dredging was conducted in 1978 by the Ocean Minerals Company (USA) in the area of a French mining claim in the Clarion-Clipperton Fracture Zone (Tropical Eastern Pacific) at a depth of about 5000 m. The nematode assemblage had not returned its initial state 26 years after the experimental dredging: the total nematode density and biomass within the dredging track were significantly lower than outside the track; the biodiversity indices showed significantly lower nematode diversity within the track; and the structure of the nematode assemblage within the track differed significantly from those in the two undisturbed sites outside the track. However, there were no significant differences in the mean body volumes of adult nematodes and adult-juvenile ratios between the track and reference sites. Parameters such as the rate of sediment restoration (which depends on local hydrological conditions) and the degree and character of the disturbance appeared to be of considerable importance for the recovery rate of the deep-sea nematode assemblages and their ability to recolonize disturbed areas. The rates of recolonization and recovery may vary widely in different deep-sea regions.     

Distribution and spatial variation of hydrothermal faunal assemblages at Lucky Strike (Mid-Atlantic Ridge) revealed by high-resolution video image analysis

Whilst the fauna inhabiting hydrothermal vent structures in the Atlantic Ocean is reasonably well known, less is understood about the spatial distributions of the fauna in relation to abiotic and biotic factors. In this study, a major active hydrothermal edifice (Eiffel Tower, at 1690 m depth) on the Lucky Strike vent field (Mid-Atlantic Ridge (MAR)) was investigated. Video transects were carried out by ROV Victor 6000 and complete image coverage was acquired. Four distinct assemblages, ranging from dense larger-sized Bathymodiolus mussel beds to smaller-sized mussel clumps and alvinocaridid shrimps, and two types of substrata were defined based on high definition photographs and video imagery. To evaluate spatial variation, faunal distribution was mapped in three dimensions. A high degree of patchiness characterizes this 11 m high sulfide structure. The differences observed in assemblage and substratum distribution were related to habitat characteristics (fluid exits, depth and structure orientation). Gradients in community structure were observed, which coincided with an increasing distance from the fluid exits. A biological zonation model for the Eiffel Tower edifice was created in which faunal composition and distribution can be visually explained by the presence/absence of fluid exits.     

Implementation of the European Water Framework Directive: Natural variability associated with the CARLIT method on the rocky shores of the Ligurian Sea (Italy)

The Water Framework Directive (WFD) 2000/60/EC, adopted by the European Community in 2000 with the goal of maintaining and improving the aquatic environments, requires that member states achieve and maintain a good ecological status of all water bodies by 2015. In the marine context, the ecological status has to be quantified applying indexes based on appropriate key biological elements, which allow the categorization of water bodies into five Ecological Status (ES) classes. The CARLIT index is a cartographic monitoring tool enabling the Ecological Quality Ratio (EQR) to be calculated using macroalgae in coastal hard bottoms as a key biological element; at present it is being applied in Spain, France and Italy. To detect actual changes of water quality and, consequently, rely on the application of indexes for the assessment of the ecological status in the marine environment, it is necessary to evaluate their sensitivity to natural variability at different temporal and spatial scales. In this study we present a first quantification of natural (spatial and temporal) variability of EQR‐CARLIT quality assessment in 2006 and 2007 along the Ligurian coast (North‐Western Mediterranean, Italy). The EQR‐CARLIT values recorded along the Ligurian coastline show that natural variability of EQR‐CARLIT is low and that it does not affect the attribution of a given stretch of coast to a particular ES class, in agreement with the major human pressures acting in the area (urbanization, river load, sea‐farming). A small‐scale variability was detected, strengthening the use of cartography of the whole rocky shore, whenever possible, or, alternatively, the assessment of the ecological status for long stretches of coast, to encompass the small‐scale variability due to local factors.