Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

Near-source noise suppression of AMT by compressive sensing and mathematical morphology filtering

In deep mineral exploration, the acquisition of audio magnetotelluric (AMT) data is severely affected by ambient noise near the observation sites; This near-field noise restricts investigation depths. Mathematical morphological filtering (MMF) proved effective in suppressing large-scale strong and variably shaped noise, typically low-frequency noise, but can not deal with pulse noise of AMT data. We combine compressive sensing and MMF. First, we use MMF to suppress the large-scale strong ambient noise; second, we use the improved orthogonal match pursuit (IOMP) algorithm to remove the residual pulse noise. To remove the noise and protect the useful AMT signal, a redundant dictionary that matches with spikes and is insensitive to the useful signal is designed. Synthetic and field data from the Luzong field suggest that the proposed method suppresses the near-source noise and preserves the signal well; thus, better results are obtained that improve the output of either MMF or IOMP.     

Relationships between southern Chilean varved lake sediments,precipitation and ENSO for the last 600 years

In this paper, the relationships between paleo-precipitation and the regional influence of El Nino Southern Oscillation (ENSO) in South America are assessed from a high-resolution calendar varve-thickness record. Two short laminated sediment cores (53 and 61 cm length) from Lago Puyehue (40° S) are analysed by continuous varve measurements through the last 600 years. The calendar varve years are determined by the occurrence of graded planktonic-rich layers. The annual sediment accumulation rates are reconstructed by using the standard varve-counting methods on thin sections. The 1980–2000 varve-thickness record is interpreted in terms of climate through correlation with limnological and local monthly instrumental climate databases. The comparison between the standardized varve thickness with the instrumental records reveals a strong correlation (r = 0.75, р = 0.07) between the total varve thickness and the austral autumn/winter precipitation. We argue that strong austral winter winds and precipitation are the forcing factors for the seasonal turn-over and phytoplankton increase in the lake sediments. During strong El Nino events the precipitation and the winds decrease abnormally, hence reducing the thickness of the biogenic sediments deposited after the winter turn-over. Our results show one significant regional maximum peak of winter precipitation (>900 mm) in the mid 20th century and a significant period with lower winter precipitation (<400 mm) before the 15th century, i.e., the late Medieval Warm Period. The first peak in the mid 20th century is confirmed by the regional precipitation database. The influence of ENSO cycles over the last 600 years is assessed by spectral analysis in Fagel et al. (2007). The possible influence of the regional volcanism and/or the seismic activity on the local climate record is also discussed. This is the sixth in a series of eight papers published in this special issue dedicated to the 17,900 year multi-proxy lacustrine record of Lago Puyehue, Chilean Lake District. The papers in this special issue were collected by M. De Batist, N. Fagel, M. -F. Loutre and E. Chapron.     

Seasonal temperatures for the past ∼400 years reconstructed from diatom and chironomid assemblages in a high-altitude lake (Lej da la Tscheppa,Switzerland)

We analysed a 42 cm long sediment record from Lej da la Tscheppa, a high-altitude lake (2,616 m a.s.l.) in the Upper Engadine valley (Switzerland) for subfossil diatoms, chironomids and pollen. The chronology of the top 21 cm of the record was established using ²¹⁰Pb analysis using a constant-rate-of-supply model, and validated with ¹³⁷Cs measurements and the content of spheroidal carbonaceous particles. A tentative chronology for the lower part of the core was obtained through extrapolation of the sedimentation rates in the uppermost part of the record. Pollen assemblages in the record reflect regional changes in forestation and land-use patterns in the Upper Engadine valley and show no evidence of significant local human activity in the lake’s catchment. Diatom assemblages record a distinct increase in planktonic taxa since the early 19th century, suggesting a decrease in the duration of ice-cover. In contrast, chironomid assemblages remained stable during a large part of the record. We applied an established chironomid-based July air temperature transfer function and a newly developed diatom-based spring air temperature transfer function to reconstruct past seasonal air temperature changes at Lej da la Tscheppa. The reconstructions indicate a diatom-inferred warming trend in spring temperatures during the past ca. 400 years, whereas chironomid-inferred summer temperatures suggest a slight cooling trend. These biota-based reconstructions are in good agreement with the centennial-scale temperature trend in an independent reconstruction of regional temperatures in the Upper Engadine region based on instrumental records and documentary proxy evidence from the Alps. Our results suggest that, in high-altitude lakes, independent chironomid- and diatom-based seasonal temperature reconstruction is possible and can be successfully used to track seasonal temperature trends.     

The Upper Middle Rhine Valley as a risk area

The Upper Middle Rhine Valley, granted the status of a World Heritage site, is well known for its unique inner narrow valley of Quaternary age with its historical legacy of numerous medieval castles and old towns. Less known is that this has always been a risk area of floods and gravitative mass movements. Up to the recent past, mainly ice floods caused enormous damage. The inhabitants of the valley were well aware that they lived in a risk area, but they had learned to handle the flood hazard. With the demise of ice floods over the last 40 years, due to climate change and because of the additional heating of the river water by power plants, the awareness of flood hazards has been much diminished, in contrast to that of potential damage by rockfalls and landslides which were also much feared in the past, though at the local level only. Still in the people’s memory is the Kaub catastrophe of March 10, 1876, when 28 persons were killed by a landslide. Nowadays, even minor rockfalls are a major threat, as they will affect the much-used traffic lines on both banks of the river, in particular the railroads. Therefore, since 2002, on behalf of German Rail (Deutsche Bahn, DB), all problematic slopes have been protected by costly steel-ring nets, although they are an aesthetic problem by UNESCO standards. The feeling of absolute safety created among the public is only subjective, though, as planners are well aware of. Moreover, the impact of modern climate change on slope stability is nearly unknown. Therefore, it is still necessary to develop a risk map for the narrow valley, with emphasis on gravitational hazards.     

Communities as the agents of commodification: The Kumbo Water Authority in Northwest Cameroon

In 1991 the people of the town of Kumbo in Northwest Cameroon expelled the national water corporation from town by force. They claimed to be retrieving a network that the community rightfully owned and they began to run the system along community lines. The paper tells the story of that water supply twice. First it reproduces the popular accounts that circulated in the 1990s that emphasize the role played by the community in the development and operation of the water supply and portray recent events as a triumph of community management. Then it retells the story using archive evidence that puts a quite different light on some of the events. It is argued that the emphasis on community management has a tendency to obscure other important aspects of the story, for example its political context and consequences. It is suggested that the widespread admiration for any initiative that is community based often results in an uncritical analysis of this form of development project. In this case the commodification of water accelerates after the community has wrested control of this public service from the state.     

The trade network of the Belt and Road Initiative and its topological relationship to the global trade network

Unimpeded trade is one of the cooperation priorities in the Belt and Road Initiative proposed by China. On 15 May 2017, the Joint Communique of the Leaders Roundtable of the Belt and Road Forum for International Cooperation reaffirmed the participants’ shared commitment to build an open economy and ensure free and inclusive trade. The Belt and Road Initiative (BRI) is not only China's new action to drive its open and global development, but also a platform for an increasing number of countries to explore free and inclusive trade and promote a universal, rule-based, open, non-discriminatory, and equitable multilateral trade system. It is therefore important to examine the topological relationship between the BRI and global trade networks. More specifically, this article first analyzes the community structure of trade networks using a community detection algorithm, and then estimates the topological relationship between different trade communities. The findings of this article are as follows. First, this research identified three trade communities and two sub-communities in the BRI trade network, in which China is the core, Russia is the sub-core of the biggest trade community, and India, United Arab Emirates, and Saudi Arabia are cores of the second trade community (South Asia-West Asia). Second, it identified five trade communities in the global trade network, centred on China, USA, Russia, India-United Arab Emirates, and Germany- Netherlands-France-Britain and other European developed countries. Third, the topological analysis indicated that in the global trade network, most BRI countries are attracted by the core nodes of the BRI regions, such as the China, Russia and India-United Arab Emirates core nodes, and have strong trade contacts with BRI countries. Most Central-East European countries are mainly attracted by Germany-Netherlands-France-Britain and other developed European countries with a low penetration of BRI trade. Although some Southeast Asian countries are incorporated into the Asia-Australia-South Africa community with China as the core, they still need to strengthen trade linkages with BRI countries.     

Potential implications of differential preservation of testate amoeba shells for paleoenvironmental reconstruction in peatlands

Testate amoebae are now commonly used in paleoenvironmental studies but little is known of their taphonomy. There is some experimental evidence for differential preservation of some testate amoeba shell types over others, but it is unclear what, if any impact this has on palaeoenvironmental reconstruction. To investigate this issue we looked at palaeoecological evidence for the preservation of different shell types. We then investigated the possible impact of selective preservation on quantitative palaeoenvironmental inference. We first used existing palaeoecological data sets to assess the vertical patterns of relative abundance in four testate amoeba shell types: (1) shells made of secreted biosilica plates (idiosomes, e.g. Euglypha), (2) idiosomes with thick organic coating (Assulina), (3) proteinaceous shells (e.g. Hyalosphenia), (4) shells built from recycled organic or mineral particles (xenosomes) (e.g. Difflugia, Centropyxis). In three diagrams a clear pattern of decay was only observed for the idiosome type. In order to assess the implications of differential preservation of testate amoeba taxa for paleoenvironmental reconstruction we then carried out simulations using three existing transfer functions and a wide range of scenarios, downweighting different test categories to represent the impact of selective test decomposition. Simulation results showed that downweighting generally reduced overall model performance. However downweighting a shell type only produced a consistent directional bias in inferred water table depth where that shell type is both dominant and shows a clear preference along the ecological gradient. Applying a scenario derived from previous experimental work did not lead to significant difference in inferred water table. Our results show that differential shell preservation has little impact on paleohydrological reconstruction from Sphagnum-dominated peatlands. By contrast, for the minerotrophic peatlands data-set loss of idiosome tests leads to consistent underestimation of water table depth. However there are few studies from fens and it is possible that idiosome tests are not always dominant, and/or that differential decomposition is less marked than in Sphagnum peatlands. Further work is clearly needed to assess the potential of testate amoebae for paleoecological studies of minerotrophic peatlands.     

Loss of preferred habitat and pollen limitation threatens reproduction in a rare mountain <Emphasis Type="Italic">Paeonia delavayi</Emphasis>

Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China. Recent agricultural land expansion, however, not only causes its dwindling population size, but also poses a severe threat to its long-term persistence. Since our knowledge of the reproductive biology of P. delavayi is very limited, and some management misconceptions have further exacerbated the already worrisome situation, the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P. delavayi, and to help correct some management misconceptions. Specifically, morphological traits of P. delavayi populations growing in two different habitats (i.e., the open area versus forest understory), including basal stem diameter, plant height and flower numbers per plant, were measured and compared with each other. A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation. The results showed that P. delavayi preferred the open habitat over forest understory as it generally grew bigger with thicker stems, and produced a higher number of flowers in the former habitat. Therefore, the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land; also, P. delavayi is pollen limited, as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments (e.g., non-supplemental self pollen, non-supplemental non-self pollen and supplemental self pollen), which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants. We suggest that human intervention might be necessary to guarantee the long-term persistence of P. delavayi as harsh alpine environment, intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.     

QUEST on DASI: a South Pole CMB polarization experiment

QUEST on DASI is a ground-based, high-sensitivity, high-resolution (ℓ_max2500) experiment designed to map CMB polarization at 100 and 150 GHz and to measure the power spectra from E-modes, B-modes from lensing of the CMB, and B-modes from primordial gravitational waves. The experiment comprises a 2.6 m Cassegrain optical system, equipped with an array of 62 polarization-sensitive bolometers (PSBs), located at the South Pole. The instrument is designed to minimize systematic effects; features include differencing of pairs of orthogonal PSBs within a single feed, a rotatable achromatic waveplate, and axisymmetric rotatable optics. In addition the South Pole location allows both repeatable and highly controlled observations. QUEST on DASI will commence operation in early 2005.