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51.
We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future. 相似文献
52.
Humans and livestock have always dwelling together, especially in rural areas. Based on a multi-method approach of ethnographic fieldwork, grounded theory and one-way multivariate analysis of variance, this paper explores human and livestock dwelling. The findings show that human and livestock dwelling form a special type of human dwelling place, with forms defined as co-, mix- and dis-dwelling. Then, from co-, mix- to dis-dwelling constructed a story line to the evolution of human and livestock dwelling forms, which further explains the entire dwelling situation of humans and livestock, including the dimensions of human dweller, livestock dweller, the rural environment and special events. Among co-, mix- and dis-dwelling significant differences existed between human and livestock individuals, the nature of rural environment, vernacularity and rural tourism development. Between co- and dis-dwelling, significant differences existed in seventeen aspects; between mix- and dis-dwelling, significant differences existed in eleven aspects; meanwhile, no significant differences existed between co- and mix-dwelling. Accordingly, this suggested that human and livestock dwelling forms can be divided into mix- and dis-dwelling. This study on humans and livestock dwelling relationship offer a new perspective on rural and animal geography. This paper is an exploratory foray into rural dwelling geography as part of the Anthrozoology study and broadens the scope of extant scholarship on human dwelling morphology, as well as analyses the spatial relationships between human and livestock dwelling. 相似文献
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WANG Luyao LIU Kai MA Yan ZHANG Yaoyao TONG Jue JIA Wuhui ZHANG Shouchuan SUN Junliang 《《地质学报》英文版》2024,98(3):801-818
Geothermal resources are increasingly gaining attention as a competitive, clean energy source to address the energy crisis and mitigate climate change. The Wugongshan area, situated in the southeast coast geothermal belt of China, is a typical geothermal anomaly and contains abundant medium- and low-temperature geothermal resources. This study employed hydrogeochemical and isotopic techniques to explore the cyclic evolution of geothermal water in the western Wugongshan region, encompassing the recharge origin, water–rock interaction mechanisms, and residence time. The results show that the geothermal water in the western region of Wugongshan is weakly alkaline, with low enthalpy and mineralization levels. The hydrochemistry of geothermal waters is dominated by Na-HCO3 and Na-SO4, while the hydrochemistry types of cold springs are all Na-HCO3. The hydrochemistry types of surface waters and rain waters are Na-HCO3 or Ca-HCO3. The δD and δ18O values reveal that the geothermal waters are recharged by atmospheric precipitation at an altitude between 550.0 and 1218.6 m. Molar ratios of major solutes and isotopic compositions of 87Sr/86Sr underscore the significant role of silicate weathering, dissolution, and cation exchange in controlling geothermal water chemistry. Additionally, geothermal waters experienced varying degrees of mixing with cold water during their ascent. The δ13C values suggest that the primary sources of carbon in the geothermal waters were biogenic and organic. The δ34S value suggests that the sulfates in geothermal water originate from sulfide minerals in the surrounding rock. Age dating using 3H and 14C isotopes suggests that geothermal waters have a residence time exceeding 1 kaBP and undergo a long-distance cycling process. 相似文献
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昆阳磷矿位于滇池西南部,为探讨该磷矿的成矿物质来源及其沉积环境,从岩相学和元素地球化学两方面对磷矿石展开了研究.研究结果显示,磷块岩矿石的主要矿物成分为胶磷矿,矿体w(P2 O5)平均含量为26.16%,磷块岩P2 O5含量由地表向地下沿倾向逐渐降低,酸不溶物含量逐渐增加,在垂直剖面上,矿区中部P2 O5含量相对上部和... 相似文献
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云南个旧南部地区元素地球化学特征 总被引:4,自引:0,他引:4
在野外调研和大量样品采集分析的基础上, 运用数学地质中对应分析和因子分析两种方法对样品和变量进行分类划分和成因解释。首先,将样品和元素进行综合计算,按第一、二因子轴作出样品-元素因子平面图,在图上划分四个象限,按象限进行分类,探讨其样品-元素分类规律。其次,按象限计算因子载荷矩阵,分析各因子轴所代表的元素共生组成规律和成矿期次。根据上述研究认为第一因子轴(F1)所代表的Sn、Cu、Pb、Cu、Zn、Au、Ag等元素为主成矿元素组合和主成矿期次,而其它因子轴所代表的Mo、W、Bi、Be等元素为次成矿元素组合和次成矿期次。成矿作用是在区域构造应力驱动力的作用下,与花岗岩侵入有关。成矿溶液除来自深源以外,还与周围基性岩体(脉)和围岩密切相关,矿液实质是一种混合矿浆,在上升运移过程中,充填在有利的构造部位和有利 的地层层位中形成矿体。 相似文献
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本义采用氢化物法分离技术,使样品溶液中的锗转变为气态氢化锗(GeH_4),达到与基体分离的目的,有效地消除了各种离子的干扰。研究并证明了在15%以上的磷酸介质中,各种酸(HCl,H_2SO_4,HNO_3,HCIO_4)度在1%-10%的范围内变化均不影响锗的测定,大大简化了操作手续,方法灵敏度高、使用范围广、准确、简便、快速。Ge的检出限为0.1μg/g,相对标准偏差<7%。可应用于地质,冶金,生物,环保,卫生,食品中微量锗的测定。 相似文献
60.
在β-γ综合测铀基础上,用大晶体、大道宽,突出~(40)K的11.46MeVγ贡献,使钾的铀当量趋近零值,可以实现含钾样中铀的准确测定。 相似文献