Probing molecular-level transformations of dissolved organic matter: insights on photochemical degradation and protozoan modification of DOM from electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Molecular-level characterization of natural organic matter (NOM) has been elusive due to the inherent complexity of natural organic mixtures and to the fact that individual components are often polar and macromolecular. Electrospray ionization (ESI) is a “soft” ionization technique that ionizes polar compounds from aqueous solution prior to injection into a mass spectrometer. The highest resolution and mass accuracy of compounds within NOM have been achieved when ESI is combined with an ultrahigh-resolution mass spectrometer such as the Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS). With this technique, individual molecules within a variety of natural organic mixtures can be detected and their elemental composition can be determined. At low mass-to-charge (m/z) ratio, the resolution is high enough to assign exact molecular formulas allowing specific components of these mixtures to be identified. In addition to molecular identification, we can now use ESI FT-ICR MS to examine molecular-level changes in different organic mixtures as a function of relevant geo-processes, such as microbial alterations and photochemistry. Here we present the results from the application of ESI FT-ICR MS to two geochemical questions: (1) the effect of photoirradiation on the molecular composition of fulvic acids and (2) the role of protozoan grazers in the modification of DOM in aquatic systems.     

Simulation of Hydrophysical Processes in Coastal Regions of the Sea

In the present work, we generalize the results of our investigations in the field of simulation of hydrophysical and ecological processes in coastal regions of various seas and some closed basins. The developed and applied mathematical models and the results of numerical experiments are briefly analyzed.     

地学研究对海岸带综合治理的部分贡献

地学是地质学、地球物理学和自然地理学的专称。运用地学手段对海岸带进行综合治理（ICZM）是当前海岸带持续开发战略面临的一项重要任务。“可持续”发展意思就是在保护环境的前提下对海岸地区及其资源进行管理，并要把它当成一个长期的具体任务来完成。要想进行ICZM必须要有广博的知识，需要了解海洋和海岸岩石的成因和特征、沉积物和地形的特征，还要了解天气和气候、海洋水体以及海洋中包含的生物等。海岸体系这些组成部分的基本地学知识是ICZM得以实施的基础，因为地学可以帮助明确再生和非再生资源以及它们对经济发展的潜在作用，从人类健康、安全和生物环境保护这两个方面弄清和确定经济发展已经和将要对环境产生的影响，特别从人类健康和工程风险这些方面弄清和确定发展过程中会有哪些限制因素和灾害；通过环境基本条件和监控变化来区分自然环境变化和人类活动导致的变化等。     

Paleoclimatic changes in southern Africa during the intensification of Northern Hemisphere glaciation: evidence from ODP Leg 175 Site 1085

Pliocene age sediments from Ocean Drilling Program Leg 175, Site 1085-A and B in the Cape Basin were analyzed to investigate the impact of the intensification of Northern Hemisphere glaciation (INHG) on the South Atlantic Benguela Current system from 4 to 2 Ma. Proxies for productivity (concentrations and mass accumulation rates of total organic carbon, carbon to nitrogen ratios, percent calcium carbonate, and percent biogenic silica) as well as weight percent sand (a proxy for preservation or winnowing) peak at 3.2, 3.0, 2.4, and 2.25 Ma. Normative calculations of allied trace and major elemental determinations indicate synchronous increases in productivity peaks, as well as high concentrations and accumulations of terrigenous sediments. Coeval increases in hemipelagic sedimentation and productivity indicators could be the result of enhanced eolian sedimentation resulting from strengthened winds, leading to elevated rates of upwelling and enhanced productivity. However, rapid burial, as indicated by high sedimentation rates, could also enhance preservation. The very high concentrations (>30%) and accumulations (up to 60 g/cm²/kyr) limit the likelihood that eolian sedimentation was the only transport mechanism, invoking an additional fluvial source. Rapid burial by either eolian or fluvial transport links these intervals of enhanced preservation and productivity with continental climate changes resulting from (1) increased winds and/or dust availability due to higher aridity in the Namibia/northern South Africa region; (2) lowered sea-level related to increased ice volume; (3) increased sediment load due to wetter conditions in the continental interior; or (4) some combination. Peaks at 3.2, 2.4 and 2.25 Ma are coincident with maximum precession, suggesting a link between hemipelagic sedimentation and enhanced monsoonal circulation over southern Africa. The Site 1085 sedimentary record during the INHG seems to be controlled by low-latitude processes linked to precession rather than hig-latitude processes.     

Underwater sounds near a fuel receiving facility in western Hong Kong: relevance to dolphins

Western Hong Kong is home to two species of marine mammals: Indo-Pacific humpbacked dolphins (Sousa chinensis) and finless porpoises (Neophocaena phocaenoides). Both are threatened in many parts of their range in southeast Asia [for example, International Biological Research Institute Reports 9 (1997), 41; Asian Marine Biology 14 (1997) 111]. In 1998, when the new Hong Kong International Airport opened in western Hong Kong, small tankers (about 100 m long, cargo capacity about 6300 metric tons) began delivering fuel to the Aviation Fuel Receiving Facility (AFRF) just off Sha Chau Island, north of the airport. Calibrated sound recordings were taken over a 4-day period from a quiet, anchored boat at distances 80-2000 m from aviation fuel delivery activities at the AFRF. From the recordings, 143 sections were selected for analysis. Narrowband spectral densities on the sound pressures were computed, and one-third octave band levels were derived for center frequencies from 10 to 16,000 Hz. Broadband levels, viz. 10-20,000 Hz. were also computed. The results showed that the Sha Chau area is normally noisy underwater, with the lowest broadband levels measured corresponding to those expected during a storm at sea (sea state 6). This background noise is believed to come largely from heavy vessel traffic in the Urmston Road to the north and east of Sha Chau and from vessels in the Pearl River Estuary to the West. The sound levels from the AFRF tankers are comparable to the levels measured from similar- and smaller-sized supply vessels supporting offshore oil exploration. The strongest sounds recorded were from a tanker leaving the AFRF at distance 100 m from the hydrophone, for which the one-third octave band level at 100 Hz was 141 dB re 1 microPa (spectrum level 127 dB re 1 microPa2/Hz) and the 10-20,000 Hz broadband level was 146 dB. At distances of 100 m or more and frequencies above 300 Hz, the one-third octave band levels were less than 130 dB (spectrum level 111 dB re 1 microPa2/Hz) and decreased with increasing frequency and distance. At distances greater than about 500 m, AFRF-associated sounds were negligible, masked by the generally high noise level of the area and attenuated by poor transmission in the very shallow water (<10 m). Because it is believed that humpbacked dolphins and finless porpoises are not very sensitive to sounds below 300 Hz, the Airport Authority Hong Kong (AA) stipulated that dedicated terminal vessels not radiate underwater sounds at spectrum levels greater than 110 dB re 1 microPa2/Hz at frequencies above 300 Hz and distances greater than 300 m. The spectrum levels at 300 Hz and higher frequencies of sounds from the tankers arriving, departing, or off-loading at AFRF were less than 110 dB re 1 microPa2/Hz even at distances of 200 m or less. The AA stipulation was met. However, it is presently unknown whether the generally strong noise levels of western Hong Kong inhibit acoustically based feeding and communication, or result in increased stress or permanent shifts in hearing thresholds.     

Characterization of interface roughness of rippled sand off Fort Walton Beach, Florida

As part of the environmental characterization to model acoustic bottom scattering during the high-frequency sediment acoustics experiment (SAX99), fine-scale sediment roughness of a medium sand was successfully measured within a 600 /spl times/ 600-m area by two methods: stereo photography and a technique using a conductivity system. Areal coverage of the two methods, representing approximately 0.16 m/sup 2/ of the sea floor, was comparable, resulting in the depiction and quantification of half-meter wavelength sand ripples. Photogrammetric results were restricted to profiles digitized at 1-mm intervals; sediment conductivity results generated gridded micro-bathymetric measurements with 1- to 2-cm node spacing. Roughness power spectra give similar results in the low-spatial-frequency domains where the spectra estimated from both approaches overlap. However, spectra derived from higher resolution photogrammetric results appear to exhibit a multiple-power-law fit. Roughness measurements also indicate that spectrum changes as a function of time. Application of statistical confidence bounds on the power spectra indicates that roughness measurements separated by only 1-2 m may be spatially nonstationary.     

Validation of a finite difference method for the simulation of vortex-induced vibrations on a circular cylinder

The Karman Vortex Street generated by a circular cylinder is investigated by the numerical solution of the compressible Navier–Stokes equations in the incompressible Mach number range (Mach<0.3) using the Beam and Warming implicit scheme. The agreement with the fully incompressible projection method (Chorin, 1968) is fairly good while convergence time is very much better. The investigation suggests that the compressible Navier–Stokes equations may be used as an efficient alternative to study incompressible flows as well. Mach numbers just below 0.3 are enough to simulate incompressible flow behavior and at the same time do not cause numerical ill-conditioning in the solution. In addition, some relevant features of the vortices generated and carried by the wake of the cylinder could be fairly well captured.     

Implications from a field study regarding the relationship between polycyclic aromatic hydrocarbons and glutathione S-transferase activity in mussels

An aluminium smelter on the west coast of Scotland discharges an aqueous effluent containing polycyclic aromatic hydrocarbons (PAHs) at the head of Loch Leven. The loch also supports two mussel (Mytilus edulis) farms. Data are presented on burdens of PAHs in the soft tissues of mussels and the effect of these contaminants on glutathione S-transferase (GST) activity in mussel hepatopancreas. GST activity is shown to be correlated with total PAH burden and also with the concentrations of certain individual PAHs. These field data show that high molecular weight PAHs are closely correlated to GST activity, whereas low molecular weight PAHs are not. This suggests that 5- and 6-ring PAHs have a more pronounced role than 2- to 4-ring compounds in inducing GST activity in mussels from Loch Leven. It is proposed that it may be more appropriate to link GST activity with 5- and 6-ring compounds only, rather than with the total PAH burden.     

Casualties in dry bulk shipping (1963–1996)

In recent years, bulk carriers have been identified with high risks of catastrophic structural failure and foundering, and with heavy loss of human life. This study, based upon Lloyd's of London casualty records, identified four risk factors that had significant, independent effect upon the likelihood of a bulk carrier foundering. The risk of foundering increased with the age of the ship, and was related to the ship's flag of registration. Most importantly, however, increased risks were found for heavy cargoes of iron ore and scrap steel or iron, and for trading routes to the Far East and from Europe to North America. Additional safety measures, in particular regarding ship design and high-risk trades, may well be necessary to reduce the high casualty rates.