Antioxidant efficacy of extracts from a variety of seaweeds in a cellular system

As a part of an ongoing search for antioxidants frodm marine sources, antioxidant activities of 24 kinds of seaweeds (4 green algae, 8 brown algae, and 12 red algae) were investigated. The seaweeds were extracted by acetone/ dichloromethane and methanol, respectively. The antioxidant properties of both extracts were evaluated using four different activity tests, including degree of occurrence of intracellular reactive oxygen species (ROS), NO, lipid peroxidation, and GSH (glutathione) in mouse macrophage Raw 264.7 cells. The levels of intracellular reactive oxygen species (ROS) and GSH were measured using 2’,7’-dichlorofluorescin diacetate (DCF-DA) and monobromobimane as fluorescence probe, respectively. Moreover, the generation of NO and lipid peroxidation products were determined by each method based on the Griess reaction and TBARS assay. Solvent extracts from seaweeds such asScytosiphon lomentaria, Prionitis cornea, Laruencia okamurae,Callophyllis japonica, Sargassum horneri, Dictyopteris divaricat a,Lomentaria catenata, Corallina confuse, Ishige okamurae, andAhnfeltiopsis flabelliformi exhibited high antioxidant activities in cellular oxidizing systems.     

Interactions between the pathogenic bacterium <Emphasis Type="Italic">Vibrio parahaemolyticus</Emphasis> and red-tide dinoflagellates

Vibrio parahaemolyticus is a common pathogenic bacterium in marine and estuarine waters. To investigate interactions between V. parahaemolyticus and co-occurring redtide dinoflagellates, we monitored the daily abundance of 5 common red tide dinoflagellates in laboratory culture; Amphidinium carterae, Cochlodinium ploykrikoides, Gymnodinium impudicum, Prorocentrum micans, and P. minimum. Additionally, we measured the ingestion rate of each dinoflagellate on V. parahaemolyticus as a function of prey concentration. Each of the dinoflagellates responded differently to the abundance of V. parahaemolyticus. The abundances of A. carterae and P. micans were not lowered by V. parahaemolyticus, whereas that of C. polykrikodes was lowered considerably. The harmful effect depended on bacterial concentration and incubation time. Most C. polykrikoides cells died after 1 hour incubation when the V. parahaemolyticus concentration was 1.4×10⁷ cells ml⁻¹, while cells died within 2 days of incubation when the bacterial concentration was 1.5×10⁶ cells ml⁻¹. With increasing V. parahaemolyticus concentration, ingestion rates of P. micans, P. minimum, and A. carterae on the prey increased, whereas that on C. polykrikoides decreased. The maximum or highest ingestion rates of P. micans, P. minimum, and A. carterae on V. parahaemolyticus were 55, 5, and 2 cells alga⁻¹ h⁻¹, respectively. The results of the present study suggest that V. parahaemolyticus can be both the killer and prey for some red tide dinoflagellates.     

Neogene exhumation of the Marrakech High Atlas (Morocco) recorded by apatite fission-track analysis

Apatite fission-track (AFT) data have been obtained along a traverse across the Marrakech High Atlas to constrain its tectono-thermal evolution. AFT ages vary between 212 ± 15 Ma and 20 ± 4 Ma. An Early Miocene AFT age accompanied by long mean track length from the central part of the chain has been interpreted as the timing of the main inversion of this region with the creation of relief because of the shortening induced by the interplay between the African and Eurasian plates. Thermal modelling of samples collected south of the South Atlas Fault Zone indicates a Middle-Late Miocene or even later cooling that has been attributed to the component of the uplift of the chain related to the thermal anomaly present beneath the Atlas Mountains.     

Effects of several salt marsh plants on mouse spleen and thymus cell proliferation using mtt assay

In the present study, we have tested the effects of 21 salt marsh plants on cell proliferation of mouse immune cells (spleen and thymus) using MTT assay in culture. The methanolic extracts of six salt marsh plants (Rosa rugosa, Ixeris tamagawaensis, Artemisia capillaris, Tetragonia tetragonoides, Erigeron annus, and Glehnia littoralis) showed very powerful suppressive effects of mouse immune cell death and significant activities of cell proliferationin vitro. Especially, the methanolic extract ofRosa rugosa was found to have fifteen times compared to the control treatment, demonstrating that Rosa rugosa may have a potent stimulation effect on immune cell proliferation. These results suggest that several salt marsh plants includingRosa rugosa could be useful for further study as an immunomodulating agent.     

A model study of influence of circulation on the pollutant transport in the Zhujiang River Estuary and adjacent coastal waters

A tracer model with random diffusion coupled to the hydrodynamic model for the Zhujiang River Estuary (Pearl River Estuary, PRE) is to examine the effect of circulations on the transport of completely conservative pollutants. It is focused on answering the following questions: (1) What role does the estuarine plume front in the winter play in affecting the pollutants transport and its distribution in the PRE ? (2) What effect do the coastal currents driven by the monsoon have on the pollutants transport? The tracer experiment results show that: (1) the pollutant transport paths strongly depend on the circulation structures and plume frontal dynamics of the PRE and coastal waters; (2) during the summer when a southwesterly monsoon prevails, the pollutants from the four easterly river inlets and those from the bottom layer of offshore stations will greatly influence the water quality in Hong Kong waters, however, the pollutants released from the four westerly river-inlets will seldom affect the water quality of Hong Kong waters due to their transport away from Hong Kong; (3) during the winter when a northeasterly monsoon prevails, all pollutants released from the eight river gates will be laterally transported seaward inside the estuary and transport westward in the coastal waters along the river plume frontal zone. However, pollutants released from the surface layer of offshore stations near or east of the Dangan Channel will be carried into the coastal waters of Hong Kong by the landward component of the westward coastal current driven by the winter northeasterly monsoon. But the pollutants from the bottom layer of the offshore stations will be carried away from the offshore by the bottom flow driven by the northeasterly monsoon. This implies that only surface-released matter from offshore stations will affect the water quality of the coastal waters around Hong Kong during the winter when a northeasterly monsoon prevails.     

The Neoproterozoic volcanic complex of the Boumalne inlier (Saghro massif,Eastern Anti-Atlas; Morocco): petrological and geochemical characteristics

Neoproterozoic volcanic series are exposed in the northern edge of the Saghro massif (Eastern Anti-Atlas, Morocco). Four volcanic rock types (basalt, andesite, dacite, and rhyolite) were distinguished in the Boumalne inlier within the so-called Saghro volcanic sequences based on petrographic and geochemical observations. Boumalne volcanic rocks contain high Al₂O₃, Fe₂O₃, Ba, Sr, Zr, Rb, and Nb contents, including calc-alkaline affinity in composition. Boumalne volcanic rocks are similar to other lower-Neoproterozoic volcanic rocks such Agouiniy formation in Sirwa inlier and in other parts of Bou-Azzer inlier. Indeed, they indicate an active subduction signature. The geochemical data show a LREE enrichment compared to HREE. The fractional crystallization has played a major role during the evolution of the magma. The less-siliceous dacitic rocks could have been formed after a low degree of partial melting of mafic parental magma source, whereas the rich-siliceous rhyolite may have been derived from dacitic magma source by a higher degree of fractional crystallization.     

The socioeconomic impact of climate-related hazards: flash flood impact assessment in Kuala Lumpur,Malaysia

Small-scale flash flood events are climate-related disasters which can put multiple aspects of the system at risk. The consequences of flash floods in densely populated cities are increasingly becoming problematic around the globe. However, they are largely ignored in disaster impact assessment studies, especially in assessing socioeconomic loss and damage, which can provide a significant insight for disaster risk reduction measures. Using a structured questionnaire survey, this study applied a statistical approach and developed a structural equation model (SEM) for assessing several socioeconomic dimensions including physical impacts, mobility disruption, lifeline facilities, health and income-related impacts. The study reveals that respondents have experienced a stronger impact on direct tangible elements such as household contents and buildings as well as direct intangible elements with β coefficients 0.703, 0.576 and 0.635, respectively, at p?<?0:001 level. The direct intangible impacts affect mobility disruption with β coefficients equal to 0.701 at p?<?0:001 level which then further cause adversity to income-generating activities with β 0.316 at significant p?<?0:001 as well. The overall model fit indices show highly acceptable scores of SRMR 0.068, RMSEA 0.055 and PClose 0.092. Thus, the SEM has successfully incorporated the socioeconomic dimensions of disaster impact and explained the impact phenomena reliably. This modeling approach will allow inclusion of various variables from different disciplines to assess hazard impact, vulnerability and resilience.

     

Molecular characterization and morphology of the photosynthetic dinoflagellate Bysmatrum caponii from two solar saltons in western Korea

Species belonging to the genus Bysmatrum are peridinoid, thecate, photosynthetic dinoflagellates. The plate formula of Bysmatrum spp., arranged in a Kofoidian series, is almost identical to that of Scrippsiella spp. Bysmatrum spp., which were originally classified as Scrippsiella spp., but were transferred to the genus Bysmatrum spp. because of separation of the intercalary plates 2a and 3a by plate 3??. Whether this transfer from Scrippsiella spp. to Bysmatrum spp. is reasonable should be genetically confirmed. Dinoflagellates were isolated from 2 solar saltons located in western Korea in 2009?C2010 and 3 clonal cultures from Sooseong solar saltons and 2 clonal cultures from Garolim solar saltons were successfully established. All of these dinoflagellates were identified as Bysmatrum caponii based on morphology analysis by light and electron microscopy. The plates of all Korean strains of B. caponii were arranged in a Kofoidian series of Po, X, 4??, 3a, 7??, 6c, 4s, 5?, 0 (p), and 24??. When properly aligned, the ribosomal DNA (rDNA) sequences of the 3 Sooseong strains of B. caponii were identical, as were those of the 2 Garolim strains. Furthermore, the sequences of the 3 Sooseong strains were 0.01% different from those of the Garolim strains. However, the sequences of SSU rDNA of these Korean B. caponii strains were 9% different from that of Bysmatrum subsalsum and > 10% from that of any other dinoflagellate thus far reported. In the phylogenetic trees generated using SSU and LSU rDNA sequences, these Korean B. caponii strains formed a clade with B. subsalsum which was clearly divergent from the Scrippsiella clade. However, this Bysmatrum clade was phylogenetically close to the Protoperidinium and/or Peridinium clades. The results of the present study suggest that Bysmatrum spp. are markedly different genetically from Scrippsiella spp..     

Evolution of sand encroachment using supervised classification of Landsat data during the period 1987–2011 in a part of Laâyoune-Tarfaya basin of Morocco

Abstract

The study anticipated to understand sand encroachment evolution through analysis of sand contribution across space and time using remote sensing in Laâyoune-Tarfaya basin, Morocco, over the period from 1987 to 2011. The assessment based on supervised classifications of Landsat imagery orthorectified data, using Maximum Likelihood (ML), Minimum Distance (MD) and Support Vector Machine (SVM) classifiers. In order to ameliorate the information, principal components analysis (PCA) and co-occurrence measurement algorithm were used for choosing bands and data transformation. Images differencing was applied on image pairs derived from classification to analyze sand encroachment evolution. All classifiers present enhanced performances, and revealed that area covered by sand was increased by 7%, 4.66% and 4.59% for ML, MD and SVM, respectively. Consequently, images differencing results confirmed that sand material increasing arise not only from coastal area contribution but also mostly from erosion of complicated sand dunes exist in the middle part of the studied area. Evaluating of the presented phenomenon dimensions and its consequences are extremely important to increase the local authorities awareness and mainly for avoiding or minimizing the consequences of the future sand dunes threats.     

Compactness,connectivity, and walking accessibility on the neighborhood level according to sustainability certifications: improvement or downgrade? A case study of Cairo,Egypt

Urban sustainability certifications (USCs) urge developers to exceed the local norms and regulatory requirements to attain sustainability. USCs are gaining international recognition as planning and policy support tools. This study aims to assess the relevance of four USCs (LEED for Neighborhood Development, BREAAM communities, CASBEE for Urban Development, and Pearl Community Rating System) in contexts outside their country of origin using Cairo Governorate as a case study. The study focuses on compactness, street connectivity, and walking accessibility as prominent components for sustainable mobility and urban form at the neighborhood level. The study examines 202 neighborhoods in Cairo in terms of compactness and then focuses on eight urban areas in different locations and with different characteristics to assess their connectivity and walking accessibility. Different analyses were performed with ArcGIS software using data about neighborhoods’ population, residential units, street networks, established buildings, buildings’ outlines and heights, and detailed uses. Results show that USCs’ indicators and thresholds are generally lenient and insensitive to the context of formal areas in Cairo Governorate, which are significantly more compact, mixed (horizontally and vertically), and connected. This study adds to the currently limited empirical evidence refuting the use of some USCs as global tools and questioning their utilization in different contexts either as they are or even through an adaptation process.