Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.     

RXTE observations of the transient sources IGR J17091-3624 and IGR J18539+0727

We present the results of our analysis of the RXTE observations for two transient sources, IGR J17091-3624 and IGR J18539+0727, in April 2003. The derived energy spectra of the sources and the power-density spectra of their light curves make it possible to classify them as low/hard-state X-ray binaries. The parameters of the power spectrum for IGR J18539+0727 lead us to tentatively conclude that the compact object in this binary is a black hole.     

A Solution of Einstein's Field Equations fora Tachyonic Gas: Possible Astrophysical Applications

In this paper we show that a change in the signs of some of the metric components of the solution of the field equations for the classical cosmic string results in a solution which we interpret as a time-dependent wall composed of tachyons. We show that the walls have the property of focusing the paths of particles which pass through them. As an illustration of this focusing, we demonstrate the results of a simple simulation of the interaction between one such tachyon wall and a rotating disk of point masses. This interaction leads to the temporary formation of spiral structures. These spiral structures exist for a time on the order of one galactic rotation. This revised version was published online in July 2006 with corrections to the Cover Date.     

APPLICATION OF GEOGRAPHICAL PARAMETER DATABASE TO ESTABLISHMENT OF UNIT POPULATION DATABASE

Now GIS is turning into a good tool in handling geographical, economical, and population data, so we can obtain more and more information from these data. On the other hand, in some cases, for a calamity, such as hurricane, earthquake, flood, drought etc., or a decision-making, such as setting up a broadcasting transmitter, building a chemical plant etc., we have to evaluate the total population in the region influenced by a calamity or a project. In this paper, a method is put forward to evaluate the population in such special region. Through exploring the correlation of geographical parameters and the distribution of people in the same region by means of quantitative analysis and qualitative analysis, unit population database (1km× 1km) is established. In this way, estimating the number of people in a special region is capable by adding up the population in every grid involved in this region boundary. The geographical parameters are obtained from topographic database and DEM database on the scale of     

EMERGY ANALYSIS OF GRAIN PRODUCTION SYSTEM IN JIANGSU AND SHAANXI PROVINCES

Efficiency and sustainability of grain production are now important in China. In this study, the grain production systems of Jiangsu and Shaanxi Provinces were compared, to analyze their efficiency and sustainability in terms of utilization of natural resources, inputs of purchased energy and materials, and outputs. Flows of energy and materials between environment and human society were identified, and the natural and human work involved in generating inputs as materials or energy were valued in terms of equivalent amount of solar energy required for their production using emergy method. The results showed environmental resources were continually playing a less important role in the systems, when inorganic subsidiary emergy inputs increased drastically while organic ones decreased or increased little. Deterioration of input emergy structure affected the systems‘ efficiency and sustainability, resulting in emergy investment ratios and environmental loading ratios increasing while yield ratios and sustainability indices decreasing. In general, efficiency and sustainability of grain production in Jiangsu are worse than those in Shaanxi. This analysis also suggested that inorganic subsidiary emergy should be introduced properly, and peasants in Jiangsu should utilize natural conditions wisely while those in Shaanxi pay enough attention to soil and surface water conservation.     

Results from several persistent train spectra intercompared

Some results following from two contemporary photographical programs (Slovakia and Japan) for persistent meteor train spectra are compared. It shows that even though both programs are not too different according to their lenses and films used, the spectral interval detected is very different. In this respect prism as a dispersion element is more favourable than the grating with blaze wavelength near 610 nm. However, some results previously assumed as typicai for presented persistent trains-e.g. the forbidden Herzberg O₂ emission, the NO₂ continuum and OH red emissions-seem to be substanciated especially due to higher and linear dispersion of those grating spectra.     

CORRESPONDENCE ANALYSIS ON IMAGES OF JIANGXI PROVINCE AS A TOURIST DESTINATION

1IN T R O D U C T IO NAn image isa setofbelief,s ideasand impressionsthatapersonholdsaboutan object(KOTLER,1991).Similar-lydefinitionistheimage of a destinationas thesum ofbelief,sideasand impressionsthatpeoplehave ofa placeor destination(CROMPTON,1979;KO…