Application of trichloroacetic acid (TCA) to extraction of soft body for the determination of tissue Cd, Cu, Pb and Zn in the prosobranch Hydrobia ulvae (Pennant)

The application of trichloroacetic acid (TCA) as a shell extractant for preparation of soft body parts with reference to tissue metal concentrations (Cd, Cu, Pb, Zn) in shellfish has been evaluated on the example of the mud snail Hydrobia ulvae, a small marine prosobranch densely present in rocky and soft-bottom habitats of the eastern Atlantic. A solution of 0.1 M TCA was tested on individuals treated according to two different protocols: (1) thawed after freezing ("non-dried") and (2) thawed and air-dried to a constant weight ("dried"). Two points were investigated in detail to improve the method: individual soft tissue dry weight and tissue metal concentration following a standard digestion method. In both instances, the results were compared with those from manually dissected snails. Conditions for total shell decalcification of 60 individuals (3-4 mm long) were 5.5 h in 20 ml of 0.1 M TCA.No differences in individual soft tissue weight were observed between the treatments, indicating good efficiency of the TCA extraction with respect to weight of soft body parts. In contrast, tissue metal concentrations varied among treatments. The TCA extraction of the dried animals had a good recovery for Cd, most likely due to the lower solubility of Cd vital cellular components (proteins and mineral concretions) from the dried tissue. Satisfactory recoveries of the tissue concentrations of Cu and Pb were obtained for the non-dried individuals. This might be related to the specific distribution of metals in the organism (namely in the digestive glands and gonads) and their different chemical reactivity with TCA after the tissue was dried. Limited susceptibility of Zn-bearing protein bindings to complexing with TCA also accounts for significantly lower concentrations of Zn in the mud snail's soft tissue that was extracted. The 0.1 M TCA solution is therefore recommended for extraction of the shells of Hydrobia ulvae for tissue determination of Cd, Cu and Pb; however the treatment protocol does affect metal recovery and thus a consistent procedure should be followed.The extracted metals from the soft tissues and shells of the mud snails (on the basis of both metal concentrations and contents) were ranked in order of increasing contribution of soft body parts to the total (shell+tissue): Pb相似文献   

Use of incipient motion data for backward erosion piping models

Backward erosion piping involves the gradual removal of granular material under the action of water flow from the foundation of a dam or levee, whereby shallow pipes are formed that grow in the direction opposite to the flow. This pipe-forming process can ultimately lead to failure of a water-retaining structure and is considered one of the most important failure mechanisms for dikes and levees in the Netherlands and the United States. Modeling of this mechanism requires the assessment of hydraulic conditions in the pipe, which are controlled by the particle equilibrium at the pipe wall. Since the pipe's dimensions are controlled by the inflow to the pipe from the porous medium, the flow through the pipe is thought to be laminar for fine- to medium-grained sands. The literature provides data for incipient motion in laminar flow, which is reviewed here and complemented with data from backward erosion experiments. The experiments illustrate the applicability of the laminar incipient motion data to determine the erosion pipe dimensions and corresponding pipe hydraulics for fine- to medium-grained sands, for the purpose of backward erosion piping modeling.     

Origin of magnetic minerals and natural remanent magnetization of basaltic rocks from Lower Silesia,Poland

Summary Two groups of basaltic rocks with normal and reversed NRM were examined. It was found that in both groups the primary NRM, synchronous with the period of rock formation, is preserved. Relations of the primary NRM to the origin and the degree of oxidation of magnetic minerals were considered in detail.     

Middle Paleozoic paleomagnetism of east Kazakhstan: post-Middle Devonian rotations in a large-scale orocline in the central Ural–Mongol belt

In order to test different hypotheses concerning the Paleozoic evolution of the Ural–Mongol belt (UMB) and the amalgamation of Eurasia, we studied Middle Devonian basalts from two localities (11 sites) and Lower Silurian volcanics, redbeds, and intra-formational conglomerates from three localities (20 sites) in the Chingiz Range of East Kazakhstan. The Devonian rocks prove to be heavily overprinted in the late Paleozoic, and a high-temperature, presumably primary, southerly, and down component is isolated at only four sites from a homoclinal section. Most Silurian redbeds are found to be remagnetized in the late Paleozoic; in contrast, a bipolar near-horizontal remanence, isolated from Silurian volcanics, is most probably primary as indicated by positive tilt and conglomerate tests. Analysis of paleomagnetic data from the Chingiz Range shows that southward-pointing directions in Ordovician, Silurian, and Devonian rocks are of normal polarity and hence indicate large-scale rotations after the Middle Devonian. The Chingiz paleomagnetic directions can be compared with Paleozoic data from the North Tien Shan and with the horseshoe-shaped distribution of subduction-related volcanic complexes in Kazakhstan. Both paleomagnetic and geological data support the idea that today's strongly curved volcanic belts of Kazakhstan are an orocline, deformed mostly before mid-Permian time. Despite the determination of nearly a dozen new Paleozoic paleopoles in this study and other recent publications by our team, significant temporal and spatial gaps remain in our knowledge of the paleomagnetic directions during the middle and late Paleozoic. However, the paleomagnetic results from the Chingiz Range and the North Tien Shan indicate that these areas show generally coherent motions with Siberia and Baltica, respectively.     

Combined analysis of precipitation and water deficit for drought hazard assessment

Abstract

The combined analysis of precipitation and water scarcity was done with the use of the Standardized Precipitation Index (SPI) and the Standardized Runoff Index (SRI), developed as a monthly, two-variable SPI-SRI indicator to identify different classes of hydrometeorological conditions. Stochastic analysis of a long-term time series (1966–2005) of monthly SPI-SRI indicator values was performed using a first-order Markov chain model. This provided characteristics of regional features of drought formation, evolution and persistence, as well as tools for statistical long-term drought hazard prediction. The study was carried out on two subbasins of the Odra River (Poland) of different orography and land use: the mountainous Nysa K?odzka basin and the lowland, agricultural Prosna basin. Classification obtained with the SPI-SRI indicator was compared with the output from the NIZOWKA model that provided identification of hydrological drought events including drought duration and deficit volume. Severe and long-duration droughts corresponded to SPI-SRI Class 3 (dry meteorological and dry hydrological), while severe but short-term droughts (lasting less than 30 days) corresponded to SPI-SRI Class 4 (wet meteorological and dry hydrological). The results confirm that, in Poland, meteorologically dry conditions often shift to hydrologically dry conditions within the same month, droughts rarely last longer than 2 months and two separate drought events can be observed within the same year.     

RC J0311+0507: A candidate for superpowerful radio galaxies in the early universe at redshift z = 4.514

A strong emission line at 6703 Å has been detected in the optical spectrum for the host galaxy (R = 23.1) of the radio source RC J0311+0507 (4C+04.11). This radio galaxy, with a spectral index of 1.31 in the frequency range 365–4850 MHz, is one of the ultrasteep-spectrum objects from the deep survey of a sky strip conducted with RATAN-600 in 1980–1981. We present arguments in favor of the identification of this line with Lyα at redshift z = 4.514. In this case, the object belongs to the group of extremely distant radio galaxies of ultrahigh radio luminosity (P ₁₄₀₀ = 1.3 × 10²⁹ W Hz^?1). Such power can be provided only by a fairly massive black hole (～10⁹ M⊙) that formed in a time less than the age of the Universe at the observed z (1.3 Gyr) or had a primordial origin.     

Convective-conductive transitions and sensitivity of a convecting ice shell to perturbations in heat flux and tidal-heating rate: Implications for Europa

We investigate the response of conductive and convective ice shells on Europa to variations of heat flux and interior tidal-heating rate. We present numerical simulations of convection in Europa's ice shell with Newtonian, temperature-dependent viscosity and tidal heating. Modest variations in the heat flux supplied to the base of a convective ice shell, ΔF, can cause large variations of the ice-shell thickness Δδ. In contrast, for a conductive ice shell, large ΔF involves relatively small Δδ. We demonstrate that, for a fluid with temperature-dependent viscosity, the heat flux undergoes a finite-amplitude jump at the critical Rayleigh number Racr. This jump implies that, for a range of heat fluxes relevant to Europa, two equilibrium states—corresponding to a thin, conductive shell and a thick, convective shell—exist for a given heat flux. We show that, as a result, modest variations in heat flux near the critical Rayleigh number can force the ice shell to switch between the thin, conductive and thick, convective configurations over a ∼10⁷-year interval, with thickness changes of up to ∼10-30 km. Depending on the orbital and thermal history, such switches might occur repeatedly. However, existing evolution models based on parameterized-convection schemes have to date not allowed these transitions to occur. Rapid thickening of the ice shell would cause radial expansion of Europa, which could produce extensional tectonic features such as fractures or bands. Furthermore, based on interpretations for how features such as chaos and ridges are formed, several authors have suggested that Europa's ice shell has recently undergone changes in thickness. Our model provides a mechanism for such changes to occur.