Microbiological and physicochemical analysis of the coastal waters of southern Brazil

The aim of this study was to assess the impact of sewage discharge on coastal waters by evaluating the influence of physicochemical parameters on the presence of enteric microorganisms in seawater samples collected from 11 beaches in Florianopolis, Santa Catarina, Brazil, over a one-year period (August 2009 to July 2010). Samples were assessed for the presence of human adenoviruses (HAdV), polyomavirus (JCPyV), hepatitis A virus (HAV), and noroviruses (HuNoV GI and GII). Escherichia coli and physicochemical parameters (salinity, temperature, pH and dissolved oxygen) were also evaluated. From the 132 samples analyzed, 55% were positive for HAdV, 51.5% for HAV, 7.5% for HuNoV GI, 4.5% for HuNoV GII, and 3% for JCPyV. E. coli levels ranged from 8 to 1325 CFU/100mL at all sites. The overall results highlight the problem of sewage discharge into coastal waters and confirm that there is no correlation between viral presence and bacterial contamination.     

青藏高原近地层热量湍流系数的实验分析

本文着重研究青藏高原近地层的湍流结构。使用梯度观测和热量平衡方法,确定了感热湍流系数(Kh)表达式中的无量纲系数1/ψmψh,并表明它与W.O.Pruitt等人的结果相当一致。本文还计算了睛天和多云条件下1米高度上感热湍流系数,最后比较了高原和低海拔平原上感热湍流系数与动量湍流系数的比值αT。     

澳大利亚吉普斯兰盆地第三纪褐煤煤岩类型的孢粉学研究

通过对澳大利亚东南部吉普斯兰盆地第三纪褐煤的5种煤岩类型的孢粉学研究,揭示出不同的煤岩类型有不同的形成环境,由浅色至深色的煤岩类型基本上对应于开阔水—沼泽—隆起泥沼的环境。     

Understanding ship-grounding impacts on a coral reef: potential effects of anti-foulant paint contamination on coral recruitment

The 184 m cargo ship Bunga Teratai Satu collided with Sudbury Reef, part of the Great Barrier Reef and remained grounded for 12 days. The ship was re-floated only 3 days prior to the November 2000 mass coral spawning. No cargo or fuel was lost but the impact resulted in significant contamination of the reef with anti-foulant paint containing tributyltin (TBT), copper (Cu) and zinc (Zn). Larvae of the reef-building scleractinian coral Acropora microphthalma were exposed to various concentrations of sediment collected from the grounding site in replicated laboratory experiments. Two experiments were performed, both of which used varying ratios of contaminated and control site sediment in seawater as treatments. In the first experiment, the influence of contaminated sediment on larval competency was examined using metamorphosis bioassays. In the second, the effect of contaminated sediment upon larval recruitment on pre-conditioned terracotta tiles was assessed. In both experiments, sediment containing 8.0 mg kg(-1) TBT, 72 mg kg(-1) Cu and 92 mg kg(-1) Zn significantly inhibited larval settlement and metamorphosis. At this level of contamination larvae survived but contracted to a spherical shape and swimming and searching behaviour ceased. At higher contamination levels, 100% mortality was recorded. These results indicate that the contamination of sediment by anti-fouling paint at Sudbury Reef has the potential to significantly reduce coral recruitment in the immediate vicinity of the site and that this contamination may threaten the recovery of the resident coral community unless the paint is removed.     

Inorganic and methylmercury: do they transfer along a tropical coastal food web?

Methylmercury (MeHg) and inorganic mercury (Hg_inorg) were evaluated in the water of a Brazilian estuary, with two size classes of plankton and seven fish species of different feeding habits. Water partition coefficients (PCs) in microplankton were fourfold higher for MeHg than for Hg_inorg; and water PCs in mesoplankton were 26 times higher for MeHg than Hg_inorg. Difference between microplankton and mesoplankton MeHg bioaccumulation factor (BAF) was higher (0.60 log units) than Hg_inorg BAF (0.24 log units), indicating that trophic transfer of MeHg between planktonic organisms is more efficient than Hg_inorg transference. MeHg concentrations, proportion of mercury as MeHg and its biotransference factors (BTFs) in the microplankton, mesoplankton and fish increased with increasing trophic level while biotic concentrations of Hg_inorg and proportion of mercury as Hg_inorg decreased thus indicating that MeHg was indeed the biomagnified species of mercury. MeHg reflected the vertical trophic guilds distribution, due to the fact that the top predator fish presented the highest concentration (0.77 μg g⁻¹ d.w.), followed by the less voracious species (0.43 μg g⁻¹ d.w.); while planktivorous fish presented the lowest concentrations (0.044 μg g⁻¹ d.w.). Hg_inorg did not present the same behavior. Results suggest that feeding habits and trophic guild are important parameters, influencing biotransference and biomagnification processes.     

Comparative effects of herbicides on photosynthesis and growth of tropical estuarine microalgae

Pulse amplitude modulation (PAM) fluorometry is ideally suited to measure the sub-lethal impacts of photosystem II (PSII)-inhibiting herbicides on microalgae, but key relationships between effective quantum yield [Y(II)] and the traditional endpoints growth rate (mu) and biomass increase are unknown. The effects of three PSII-inhibiting herbicides; diuron, hexazinone and atrazine, were examined on two tropical benthic microalgae; Navicula sp. (Heterokontophyta) and Nephroselmis pyriformis (Chlorophyta). The relationships between Y(II), mu and biomass increase were consistent (r(2)0.90) and linear (1:1), validating the utility of PAM fluorometry as a rapid and reliable technique to measure sub-lethal toxicity thresholds of PSII-inhibiting herbicides in these microalgae. The order of toxicity (EC(50) range) was: diuron (16-33nM)>hexazinone (25-110nM)>atrazine (130-620nm) for both algal species. Growth rate and photosynthesis were affected at diuron concentrations that have been detected in coastal areas of the Great Barrier Reef.