The development of a rapid screening technique to measure antibiotic activity in effluents and surface water samples

Use of antibiotics in medicine, farming and aquaculture results in a continual supply of these pharmaceuticals and their breakdown products reaching the environment. A method has been developed to use for screening environmental samples to establish whether they contain antibiotics. The assay is based on a commercially available test kit and uses some modifications of the test procedure and SPE techniques to deliver a fast, semi-quantifiable result. Development of the assay and results from spiked and environmental samples are discussed.     

The effect of Prestige oil ingestion on the growth and chemical composition of turbot otoliths

Juvenile turbot (Scophthalmus maximus) were kept in captivity and were fed a prepared food contaminated with five different concentrations of seawater-accommodated fuel oil from 2.4+/-0.35 to 48.2+/-2.2 mg g(-1) food, with a control group receiving uncontaminated food. The growth and survival of individually tagged fish (N=202) were measured after a six-week treatment period. The otolith growth rate was measured and otolith composition was determined before and after the treatments using LA-ICPMS. Fish and otolith growth were negatively affected by the fuel oil treatment, and the response decreased with the level of contamination. Otolith growth and element incorporation peaked at mid level exposures and decreased at the highest level. The otolith elemental composition reflected the presence of some elements in the Prestige fuel that may have been incorporated through the diet into the otolith.     

A comparison of acute and chronic toxicity tests used to examine the temporal stability of a gradient in copper tolerance of Hediste diversicolor from the Fal estuary, Cornwall, UK

The aim of this study was to use two different toxicity tests to verify the existence of a gradient in tolerance along Rostronguet Creek. Hediste diversicolor was collected from five populations in the Fal estuary previously shown to vary in copper tolerance. Exposure to 4 mgL(-1) of copper in an acute assay demonstrated that Mylor Creek worms were sensitive (LT(50) 86 h) and the tolerance of Rostronguet Creek worms increased moving upstream from the mouth of the creek (LT(50)s 100-258 h). There was no significant difference in tolerance between Mylor worms and worms from the mouth of Rostronguet Creek. This is in agreement with a previous study [Grant, A., Hateley, J.G., Jones, N.V., 1989. Mapping the ecological impact of heavy metals on the estuarine polychaete Nereis diversicolor using inherited metal tolerance. Marine Pollution Bulletin 20, 235-238] and demonstrates temporal stability of the gradient. Copper tolerance was also measured using a chronic toxicity test run for 90 d using step-wise increases in challenge concentration. A significant difference in tolerance was shown between populations from Mylor Creek and those at the mouth of Rostronguet Creek, which has not been reported previously. Experimental protocol was therefore an important factor in detecting population variation in tolerance.     

Exploring suitability for tropical crop cultivation in Hainan Island by SOTAL methodology

1 Introduction Land degradation is a major threat to environmental and agricultural quality of land[1] and is directly related to food security issues. An instrument for decision-makers to plan or stimulate allocation of land to users that can be sustained and are economically viable and socially acceptable. Different kinds of land are unequally suited to various uses. Land evaluation is the assessment of the suitability of a tract of land for a specified kind of use and it provides objective…     

我国煤矿区生态环境现状及综合整治对策

本文总结了国煤矿开发对生态环境破坏的总体状，指出了我国煤矿区生态环境的特征，即华北地区和华东部分地区煤矿开发高强度与煤矿区人多地少的矛盾，西部地区煤储量丰富区与生态环境脆弱性的矛盾玫我国广大南方煤矿开发分散与煤质差、污染重的矛盾。     

The emergence of life from iron monosulphide bubbles at a submarine hydrothermal redox and pH front

Here we argue that life emerged on Earth from a redox and pH front at c. 4.2 Ga. This front occurred where hot (c. 150 degrees C), extremely reduced, alkaline, bisulphide-bearing, submarine seepage waters interfaced with the acid, warm (c. 90 degrees C), iron-hearing Hadean ocean. The low pH of the ocean was imparted by the ten bars of CO2 considered to dominate the Hadean atmosphere/hydrosphere. Disequilibrium between the two solutions was maintained by the spontaneous precipitation of a colloidal FeS membrane. Iron monosulphide bubbles comprising this membrane were inflated by the hydrothermal solution upon sulphide mounds at the seepage sites. Our hypothesis is that the FeS membrane, laced with nickel, acted as a semipermeable catalytic boundary between the two fluids, encouraging synthesis of organic anions by hydrogenation and carboxylation of hydrothermal organic primers. The ocean provided carbonate, phosphate, iron, nickel and protons; the hydrothermal solution was the source of ammonia, acetate, HS-, H2 and tungsten, as well as minor concentrations of organic sulphides and perhaps cyanide and acetaldehyde. The mean redox potential (delta Eh) across the membrane, with the energy to drive synthesis, would have approximated to 300 millivolts. The generation of organic anions would have led to an increase in osmotic pressure within the FeS bubbles. Thus osmotic pressure could take over from hydraulic pressure as the driving force for distension, budding and reproduction of the bubbles. Condensation of the organic molecules to polymers, particularly organic sulphides, was driven by pyrophosphate hydrolysis. Regeneration of pyrophosphate from the monophosphate in the membrane was facilitated by protons contributed from the Hadean ocean. This was the first use by a metabolizing system of protonmotive force (driven by natural delta pH) which also would have amounted to c. 300 millivolts. Protonmotive force is the universal energy transduction mechanism of life. Taken together with the redox potential across the membrane, the total electrochemical and chemical energy available for protometabolism amounted to a continuous supply at more than half a volt. The role of the iron sulphide membrane in keeping the two solutions separated was appropriated by the newly synthesized organic sulphide polymers. This organic take-over of the membrane material led to the miniaturization of the metabolizing system. Information systems to govern replication could have developed penecontemporaneously in this same milieu. But iron, sulphur and phosphate, inorganic components of earliest life, continued to be involved in metabolism.     

淡水湖泊沉积物中有机硫的固定作用及C／S比值的意义

本文通过取自阿尔卑斯山边湖泊（苏黎世湖和日内瓦湖）的两个沉积柱的对比分析，阐明了硫在淡水沉积物中的固着途径。尽管这两个湖泊中湖水硫酸盐浓度相差三位以上，但固定在沉积物中的硫含量是相等的。沉积硫的最大深度达１％（干重），与现代近海岸相沉积物的硫含量相似。也表现在Ｃ有机／Ｓ总比值上，该比值较低，为２．５－８。对硫储体的分析表明，有机硫化合物是沉积硫的主要成分，在苏黎世湖和日内瓦湖中分别占总数的８０％和