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251.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
252.
刀额新对虾染色体核型及细胞核DNA含量 总被引:9,自引:0,他引:9
以刀额新对虾胚胎、无节幼体、卵巢、精巢等为材料 ,空气干燥法制备染色体 ,并初步进行核型分析。结果表明 ,刀额新对虾的染色体数目为 2n=78,n =39,核型为 2n=78=40M 1 0SM 1 4ST 1 4T。以刀额新对虾血淋巴、卵巢、肌肉、鳃为材料 ,以鸡血细胞为DNA标准 (2 50pg/ 2c) ,使用PartecCCAⅡ型流式细胞仪测定了刀额新对虾细胞的基因组DNA含量 ,其值为鸡血细胞的 1 75倍 ,绝对含量为 4 375pg/ 2c。 相似文献
253.
Xie Simei Hao Chunjiang Zou Bin Jing Qiyi Liao Xiaohong Sheng Xiaowen Zhang Canrong 《海洋学报(英文版)》2000,19(1):35-46
INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo… 相似文献
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长江口拦门沙地区的泥沙运动规律 总被引:12,自引:0,他引:12
依据1988年7月和12月水文观测资料,通过流,盐,沙的综合分析,认识到长江口拦门沙地区的泥沙运动有与河口其他地区不同的特点,泥沙输移在水平面上和垂直面上存在着多种循环,潮流和盐水截留大量泥沙积聚的拦门沙地区,河口在盐淡水交锋地带,除了涨,落急时段外,发现在转流时期泥沙也可能产生再悬浮,从而形成一个潮周期中出现3次或4次再悬浮的特殊规律。 相似文献
256.
本文介绍了厦门岛市区机动车流遥感监测的实施目的和方法,通过航空影像有关题信息解译与实例、专题图件编制以及统计资料的对比分析,认为:目前厦门岛市区交通主要存在着机动车增长过速、道路狭窄和会计室场少且分布不合理等三个方面的问题,文中提出了相应的建议。体现了航空遥感对于中、大比例尺城市动态监测快速有效的特点。 相似文献
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The sediments of the eastern Mediterranean basin contain a series of organic-rich sapropels intercalated with organic-poor nannofossil oozes. Until recently the timing of the onset of sapropel formation was not known accurately because of the low resolution achievable by conventional radiocarbon dating. Compilation of all available 14C-AMS dates show that the base of S-1 (the most recent sapropel) was initiated 8800 years B.P. (14C age corrected by 400 years for reservoir effect) under a 500 m water column and moved progressively into deeper water reaching depths of 3500 m at 8200 years B.P. The linear correlation between the age of S-1 onset and water depth suggests that formation of sapropels moved into deeper water at a rate of 1000 m/200 year. A model is suggested in which export production which sank below the well-mixed surface layers (500 m) was respired consuming dissolved oxygen in the Levantine deep water until a threshold value was reached when sapropels began to be preserved in the sediment. This resulted in a progressively deepening oxygen minimum zone with time until eventually the entire deep water in the basin was oxygen depleted. Assuming that the threshold value for sapropel formation was complete anoxia, it was calculated that primary productivity in the basin during the deposition of S-1 was a factor of 5 greater than that found at present. 相似文献