Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

ASCA Observation of the Quiescent X-Ray Counterpart to SGR 1627-41

We present a 2-10 keV ASCA observation of the field around the soft gamma repeater SGR 1627-41. A quiescent X-ray source, whose position is consistent both with that of a recently discovered BeppoSAX X-ray source and with the Interplanetary Network localization for this soft gamma repeater, was detected in this observation. In 2-10 keV X-rays, the spectrum of the X-ray source may be fit equally well by a power-law, blackbody, or bremsstrahlung function, with unabsorbed flux approximately 5x10-12 ergs cm-2 s-1. We do not confirm a continuation of a fading trend in the flux, and we find no evidence for periodicity, both of which were noted in the earlier BeppoSAX observations.     

坚定的信心从何而来？——对当下中国迎战经济衰退的另类思考

当2009年元旦来临之际,国务院总理温家宝来到了青岛,看望并慰问广大的干部群众,鼓励大家面对当前的经济困境要坚定信心,迎难而上,创造新业绩。温总理抓住了解决当前中国应对经济衰退问题的核心,抓住了根本。对一个“后发的工业化大国”而言,战胜当下的经济困难,中国缺少的不是“金钱”,而是“人心”。问题的关键在于树立坚定的信心。——题记     

应用脉冲激发光源提高光谱分析准确度的原因

关于应用脉冲激发光源提高光谱分析的准确度的合理性方面,以前[1,2]已经指出过,并且在借助于活化同位素的情况下,获得了关于原子在这种光源的电极隙间空中分布的初步知识。现在的工作是这些研究的继续,其目的是更系统地研究脉冲电弧的最重要的物理参数,它们可以说明脉冲电弧对测定准确度的影响以及查明应用这种光源比应用普通的直流或交流电弧的准确度高的原因。光源温度测量的准确度已经作了改进,并进行了脉冲光源中电流强度的估计,以及取得了     

金刚石钻头性能的测试设备

测试新设计的现有的钻头、是发展钻进技术的基础。虽然现场测试是获得现场数据的唯一途径,但实验室测量可以在很宽范围的工作条件下最经济地获取实验数据。A。Ersoy和M.D Waller的文章,介绍了诺丁汉大学的一种在实验室测试岩石钻头的钻机。设计该钻机的目的是为了对各种不同岩石的众多钻头提供严格的测试平台。该钻机能够提供0－2000r/min的转速,最大4000kg的载荷和200Nm的扭矩。钻进可以在恒定进给或恒定载荷条件下进行。机器构架可以容纳高1m的试件。采用专门设计的软件,所有关键数据都能自动录入计算机,并自动形成报告书。软件可连续显示有效特征参量,如钻进比能量。常用功能还包括对实验室进程中钻头磨损的监控。     

1959-1965水文地質工作的基本任务

正 为了完成工苏联共产党第廿一次代表大会给地质部门提出的任务,必须进行地质和水文地质测量,以便为有成效地进行各种矿物原料（其中包括象地下水这样的重要的矿产）的普查与勘探工作,提供必要的科学依据。但是苏联的领土在水文地质方面研究得不平衡和不充分,比地质方向要差得多。尽管苏联领土的水文地质研究程度是比较浅的,     

西北非陆缘的大规模块体坡移：被动陆缘块体坡移的一般推论

西北非海域的陆缘地貌因受到顺陆坡而下和平行于陆坡两个方向沉积物运移作用的相互影响而有很大改变。在几个航次的调查中,通过地球物理、沉积学和地球化学方法,对位于12°～26°N之间的塞内加尔和毛利塔尼亚海域的沉积物运移作用进行了调查。测深和高分辨率地震资料被用于研究再沉积的延伸范围以及内部结构。根据这些测量结果进行了沉积物取芯,并进行了沉积学和地球化学分析。     

可持续土地管理的宏观经济政策及组织措施

今年四月,联合国可持续发展委员会召开了第八次会议(CSD—8),中心议题是土地资源可持续管理及农业。21世纪议程第10章“土地资源的统筹规划和管理”、第11章“森林毁灭的防治”、第12章“脆弱生态环境的治理:防沙治旱”、     

Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea

Heat shock proteins 70 (hsp70) are known to be induced by a great variety of chemical stressors. The effects of different environmental contaminants, which were identified in sediments of the North Sea and the Baltic Sea as main contaminants or as contaminants of special toxicological interest, on the extent of expression of hsp70 in a permanent cell line Epithelioma papulosum cyprini (EPC) were investigated. Concentrations of the single contaminants leading to elevated hsp70 levels were compared to the respective concentrations of these contaminants encountered in the sediment extracts thus allowing an assessment of their contribution to the toxicity of these sediments. Analysis of hsp70 was performed using western blotting techniques with subsequent comparative quantification by densitometry. Eleven of thirteen contaminants have shown a dose-dependent increase in hsp70 levels. An expression of hsp70 was observed in most cases between 0.1 and 10 microg/mL test solution. Some of the investigated stressors led to extremely high hsp70 contents even at low concentrations. However, in most cases contaminant concentrations in sediments were too low to cause an effect. For this reason, effects of the tested sediments are attributed to other contaminants or rather to mixtures of stressors.