Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

Benthic infaunal communities around two artificial reefs in Mamala Bay, Oahu, Hawaii

Non-fishery use of artificial reefs has been given attention in recent years. The primary concern associated with non-fishery artificial reefs is their effects to the surrounding ecosystems. This study examined the infaunal communities around two non-fishery artificial reefs (the sunken vessels YO257 and Sea Tiger) in Mamala Bay, Hawaii. Infaunal community structures at these artificial reefs were relatively similar to one at a nearly natural patch reef. A large amount of basalt gravel around YO257 associated with its deployment operation possibly had an effect on the surrounding community by increasing pore space. Polychaete assemblages were compared with existing data throughout the bay, and this revealed that the variation in sediment grain sizes and depths seemed to play some role in structuring the polychaete communities. Nevertheless, the infaunal communities around the artificial reefs were typical and within the range of natural variation in Mamala Bay, supporting their beneficial uses in ecotourism.     

印度西南海岸Kannur区红树林沉积物的水动力反应

对Ｋａｎｎｕｒ红树林的地表和岩心沉积物的粒度特征和统计参数做过研究。向陆地带、中间过渡带和浅水剖面的砂、泥含量几乎为常量。地表沉积物平均粒度变化范围在１．８３和５．５６之间,岩心沉积物的变化范围在２．３和５．５６之间。沉积物普遍分选差至极差、...     

全球石油资源估计及对21世纪石油产量的预测

将地球沉积盖层作为统一的系统,对全球常规可采石油资源进行概率估计,并对21世纪末之前的全球石油产量进行预测。结果表明全球石油产量最有可能在2020—2030年间达到高峰,每年42～47亿吨。届时主要的产区将在波斯湾、西西伯利亚和东西伯利亚,上游部门的注意力将转向北极陆架。至少到2040年前,年产量将维持在42—45亿吨水平。     

西北非陆缘的大规模块体坡移：被动陆缘块体坡移的一般推论

西北非海域的陆缘地貌因受到顺陆坡而下和平行于陆坡两个方向沉积物运移作用的相互影响而有很大改变。在几个航次的调查中,通过地球物理、沉积学和地球化学方法,对位于12&#176;～26&#176;N之间的塞内加尔和毛利塔尼亚海域的沉积物运移作用进行了调查。测深和高分辨率地震资料被用于研究再沉积的延伸范围以及内部结构。根据这些测量结果进行了沉积物取芯,并进行了沉积学和地球化学分析。     

南极Scotia海Dove山脉形成的火山作用及其岩石学、地球化学和构造环境

本研究对来自DOVE盆地（Scotia海南部）中部火山山脊的拖网样品进行了详细的岩石学、地球化学分析,辅以K—Ar年代学分析。研究区水深2000～3000m,地理坐标范围为59&#176;21～23′S和42&#176;27～31′w。本研究的目的在于,通过比较研究区火成岩和形成Scotia、Antartic—Phoenix洋中脊及南设得兰群岛的火山岩之间的地球化学特征和同位素特征,厘定研究区岩浆形成的地球动力学背景以及岩浆作用的绝对年龄。     

1nm锰矿相的金属阳离子交换能力的研究

人工合成的1nm锰矿相和铜、钴、镍等二价金属阳离子的交换实验表明,1nm锰矿相具有强烈的金属阳离子交换能力.正是这种金属阳离子的交换能力,使得大洋多金属结核大量地富集了铜、钴、镍等有价金属元素.1nm锰矿相对几种金属阳离子的交换的优先顺序为:Cu>Co>Zn≥Ni>Ca>Mg.多金属结核中镍的含量往往高于铜、钴、锌等元素,但在交换顺序中它却低于铜、钴、锌等,这种不一致性说明结核成矿后期的金属离子交换并非是有价金属结合进结核的唯一途径.多金属结核富含铜、钴、镍等有价金属可能是结核初始成矿和成矿后期金属离子交换等多种因素共同作用的结果.     

南极为什么这么寒冷？：科学家们继续研究南极冰盖的历史

南极为什么这么寒冷？科学家们继续研究南极冰盖的历史ＡａｒｏｎＷｏｏｄｓ南极冰盖深深地影响着世界气候。它使深海冷却并且控制循环,影响这些深海的化学平衡而且对世界范围的海平面和温度的变化有主要影响。大洋钻探计划（ＯＤＰ）正在对南极大陆的边缘海域进行为期两...