Mechanistic evaluation and reaction pathway of UV photo-assisted Fenton-like degradation of progesterone in water and wastewater

The mechanisms and reaction pathway of UV photo-assisted Fenton-like degradation of progesterone in water and wastewater were investigated. The reaction followed the pseudo-first-order kinetics for both the dark Fenton-like and UV photo-Fenton-like processes. The reaction kinetics of the UV photo-assisted process improved with the presence of humic acid (HA) in wastewater, due to the formation of Fe(III)-HA complexes which enhanced Fe(II) production by a ligand-to-metal charge transfer. The UV photo-assisted process reduced the amount of ferric required to completely degrade progesterone by 60 % and lowered the activation energy to 42 kJ/mol compared to 104 kJ/mol for the dark Fenton-like process. Complete degradation of progesterone was achieved through a multi-step process involving several intermediate compounds.     

Assessment of the selected trace metals in relation to long-term agricultural practices and landscape properties

Understanding of the landscape response to agricultural practices mainly in relation to soil trace metals requires particular attention. Consistent with this, the trend and possible pollution of total and DTPA fraction of Mn, Zn, Cu, and Cd in the agricultural soils developed on different landscape positions involving piedmont alluvial plain (PAP), river alluvial plain (RAP), plateau (PL), and lowland (LL) were investigated. The content of the metal in different soil profiles, grouped by landscape positions, varied in the following orders: total and DTPA-Mn as LL > PAP > RAP > PL, total Zn and Cu as PAP > RAP > LL > PL, total Cd as RAP > PAP > PL > LL, DTPA-Zn as RAP > PAP > PL > LL, and DTPA-Cu as RAP > LL > PL > PAP. A wide variation in the total fraction of Mn (89–985 mg kg^?1), Zn (24–152 mg kg^?1), Cu (8–27 mg kg^?1), and Cd (0.6–1.7 mg kg^?1) and in the DTPA fraction of Mn (1.2–11 mg kg^?1), Zn (0.3–4.4 mg kg^?1), Cu (0.3–3 mg kg^?1), Cd (0.03–0.09 mg kg^?1) observed as a result of the effects of agricultural practices and landscape properties. The values of both total and DTPA-extractable Mn, Zn, and Cu were enriched in the AP horizon probably due to anthropogenic activities particularly successive use of agrochemical compounds and manure during numerous years. Using soil pollution indices [single pollution (PI) and comprehensive pollution (PIN)], the study soils were categorized mainly as low to moderate pollution and Zn was identified as the major element affecting on the yield of these indices.     

Influence of varying nitrogen levels on lipid accumulation in <Emphasis Type="Italic">Chlorella</Emphasis> sp.

Microalgal lipids can be enhanced through varying nitrogen (N) content, and limited supply of nitrogen source seems to be valuable approach for increased lipid accumulation in microalgae. In this study, Chlorella sp. IM-02 was observed under fluorescence microscope for increased number of lipid bodies under nitrogen scarcity. Fourier transform infrared spectroscopy was used to determine spectral changes due to varying lipid content under nitrogen-starved (N₀, without sodium nitrate), nitrogen-limited (N_0.1, N_0.25, N_0.5 and N_1.0 representing 0.1, 0.25, 0.5 and 1.0 g/L of sodium nitrate, respectively) and nitrogen-sufficient (N_1.5, i.e., 1.5 g/L sodium nitrate) setting. Chlorophyll content was also monitored under these conditions as growth indicator. Various biochemical components viz. total carbohydrates, total proteins and total lipids were also estimated under varying nitrogen levels spectrophotometrically. On fourth day itself, maximum lipid productivity was observed in case of N_0.5, which is having one-third of nitrogen concentration present in original growth media, BG-11. This concludes N_0.5 as suitable nitrogen provision for better production of lipids in Chlorella sp. IM-02 without much compromising the biomass production as both growth and lipid quantity are key parameters affecting the lipid productivity of any microalgal strain.