Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara

Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus, and the environment.     

Antioxidant system responses in two co-occurring green-tide algae under stress conditions

Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera(Müller) J. Agardh has been identified as the bloom-forming alga,co-occurring with U. intestinalis. We observed distinct strategies for both algal species during green tides. U. prolifera exhibited a high abundance initially and then decreased dramatically,while U. intestinalis persisted throughout. The antioxidant system responses of these two macroalgae were compared in the late phase of a green tide(in-situ) and after laboratory acclimation. Lipid peroxidation and antioxidant system responses differed significantly between the two. Malondialdehyde and hydrogen peroxide contents increased significantly in-situ in U. prolifera,but not in U. intestinalis. In U. prolifera,we observed a significant decrease in total antioxidant ability(T-AOC),antioxidant enzymes(SOD and Apx),and non-enzyme antioxidants(GSH and As A) in-situ. U. intestinalis showed the same pattern of T-AOC and SOD,but its Gpx,Apx,and GSH responses did not differ significantly. The results suggest that U. prolifera was more susceptible than U. intestinalis to the harsh environmental changes during the late phase of a Yellow Sea green tide. The boom and bust strategy exhibited by U. prolifera and the persistence of U. intestinalis can be explained by differences in enzyme activity and antioxidant systems.     

对干部教育培训针对性有效性问题的调查与思考

正党的十八大以来,按照中央和省委、省政府的部署,青海省国土资源系统先后开展了党的群众路线教育实践活动,"三严三实"专题教育、"两学一做"学习教育以及从严治党和党风廉政建设等系列教育活动。这些教育培训涉及面广,有党政领导干部、技术管理干部、基层党务干部、县处级后备干部等,也有基层业务骨干、地勘测绘一线职工等;在教育培训内容上,有政治理论学习、坚定理想信念教育、政策法律学习、专业     

栾川县国土资源局廉政文化建设成效显著

几年来,栾川县国土资源局廉政文化建设常抓不惚,成效显著。自2007年9月份该局被县纪委确定为“廉政文化进机关”工作试点单位后,局党组高度重视,采取全方位、多层次、高标准、深内涵的活动方式,抓好四项工作,实现机关作风五个转变。最近,又被洛阳市纪委、监察局授予市级“廉政文化进机关”示范单位。     

Genetic study of Kelp “901” strain

Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain, genetic study on variation was conducted to its parents and offsprings of F6, F7,Fs, and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes, 297 loci ranging from 200 to 3 000 bp were obtained in the average of 9,9 loci for each primer, This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.321 2-0.476 7, and the maximum was between F7 and Fs (0.476 7). Identity analysis showed that F6 generation was more close to the female parent (0.659 3), and F7 generation was more close to the male parent (0.5788). To the sporophytes study in 24 selected primers for RAPD amplification, 191 loci ranging from 230-2 800 bp were obtained, in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.223 9),female parent (0.107 2), F6 (0.216 4), F7 (0.228 6), F8 (0.229 6) and F9 (0.317 2). The nearest genetic distance was 0.083 5 (Fs, F9). Total heterozygosity (HT) ofF6, F7, F8 and F9 generations was 0.318 6, the average heterozygosity (Hs) for F6,F7, F8 and F9 generations was 0.248 0, and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selecting Laminaria.     

利用窄线宽激光测量锶原子束速率分布

利用多普勒频移效应,通过线宽2MHz窄线宽激光与锶原子束在大角度作用下的荧光信号分析,测量了锶原子束速率分布。比较和分析表明,理论结果与实验结果符合较好。     

关于HD94033的双星假设

在1981年2月至1984年4月间,我们对矮造父变星HD94033进行了4个观测期的观测。综合考察跨度为10年的观测资料,我们发现了它的O-C随周数的规律性变化。在研究了这种变化后,我们得到HD94033可能是一个双星系统的结论。     

不同谱线的太阳单色光观测

本文利用紫金山天文台光谱仪加配太阳像和底片同时移动的同步装置,在底片盒前加可以调节的狭缝,旋转光栅,扫描太阳单色像,得到氢 H_α(λ6563)、氢 H_σ(λ4202)、钠 D_2(λ5896)、氦 H_el(λ5876)(即D_线)、铁 FeⅠ(λ6302A)、钙 C_aⅡK(λ3934)等谱线以及连续光潜区的太阳单色像。从单色像照片上可以看出不同谱线单色像有明显的不同。     

小牛煤矿晚二叠世含煤地层沉积环境与聚煤规律

小牛煤矿位于贵州省内水城矿区的格目底向斜东段北翼,含煤地层为晚二叠世龙潭组,含煤性好。通过对小牛煤矿含煤地层及其含煤性的研究,确定了研究区的沉积环境是海陆过渡相中的三角洲沉积类型,并初步建立了沉积模式,龙潭早期和晚期均有较好的聚煤作用发生并叠加,是聚煤的最佳场所,含煤性最好。