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91.
The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy. 相似文献
92.
JoseM.Carcione UmbertaTinivella 《海洋地质》2002,(2):23-40,75
我们将浓度和压力作为一个函数得到冰和含气体水合物的地震波速度。同以往以简单的慢度和平均模量及双相模型为基础的理论不同,我们使用了Biot类型的三相理论,这一理论考虑了二种固体(颗粒和冰或笼形物)和一种液体(水)的存在,以及一个含气和水的多孔隙基质。对于固体的Berea砂岩,低于0℃时的纵波理论估计值过低,在含有冰-颗粒相互作用和颗粒胶结时与实验数据产生了好的吻合。严格地讲,水的比例与温度紧密相关。假定已知孔隙的平均半径和标准偏差,那么在一给定温度下的波速拟合就能对整个温度范围的速度进行预测。通过一个粘弹性的单相结构模型来计算反射系数。本文的分析是针对游离气带的顶部(位于含气体水合物的沉积层下)和底部(上履于饱含水的沉积层)进行的。如果似海底反射仅仅是由于胶结气体水合物与含游离气沉积层的分界面造成,我们可以得出结论:(1)在一定的气体饱和度下,很难估算低浓度情况下气体水合物数量。然而,水合物的高、低浓度还是可以区分的,因为它们分别呈现正和负的异常;(2)游离气和饱和度可以从反射振幅中而不是从异常类型中获取;(3)P波转化为S波的反射系数是指示高含量的气体水合物和游离气一个较好指示。另一方面,对于未胶结的沉积物,振幅随偏移距变化曲线总是为正。 相似文献
93.
94.
Fuzzy Optimum Model of Semi-Structural Decision for Lectotype Optimization of Offshore Platforms 总被引:8,自引:0,他引:8
In the process of concept design of offshore platforms, it is necessary to select the best from feasible alternatives through comparison and filter. The criterion set, used to evaluate and select the satisfying alternative, consists of many qualitative and quantitative factors. Therefore, the selection is a problem of multicriteria and semi- structural decision- making. Different from traditional methods in semi- structural decision- making, a new framework and methodology is presented in this paper for evaluation of offshore platform alternatives. First, the criterion set is established for the evaluation of alternatives. Next, the approach is studied to construct the relative membership degree matrix, in which both qualitative and quantitative factors are consistent with the uniform calculating standard. And then a new weight-assessing method is developed for calculation of the weights based on the relative membership degree matrix. Finally, a multi-hierarchy fuzzy optimum model is adopted to select t 相似文献
95.
The effects of different lateral confinement stress on the fatigue behavior of and cumulative damage to plain concrete are investigated experimentally. Eighty 100 mm x 100 mm x 100 mm specimens of ordinary strength concrete are tested under constant- or variable-amplitude fatigue loading and lateral confinement pressure in two orthogonal directions. A fatigue equation is developed by modifying the classical Aas-Jakobsen S-N equation for taking into account the effect of the confined stress on fatigue strength of plain concrete. The results of variable-amplitude fatigue tests indicate that the linear damage theory proposed by Palmgren and Miner is unreasonable in the biaxial stress state. A nonlinear cumulative damage model that could model the effects of the magnitude and sequence of variable-amplitude fatigue loading and lateral confinement pressure is proposed on the basis of the evolution laws of the residual strains in the longitudinal direction during fatigue tests. The residual fatigue life predic 相似文献
96.
两株琼胶酶高产细菌的筛选和鉴定 总被引:4,自引:1,他引:4
从海洋环境中筛选得到两株琼胶酶的高产菌株,通过形态观察和生理生化反应,确定它们属于弧菌属,通过BIOLOG细菌鉴定系统鉴定,并同弧菌属标准菌株分析比较,确定上方宝剑两株菌都是塔式弧菌(Vibrio tubiashii),这两株菌在碳源利用方面存在差别。 相似文献
97.
The effect of the cone spacing of a conieal structure on the ice force is studied by model experiments. The ice force reduction coefficient presented in this paper expresses the relationship between the ice force and the arrangement of cones. The experiments prove that the mode of the ice failure before the boundary of upward-downward bending cone (UDBC) is crushing. A conclusion can also be drawn from the experiments that the ice force on the boundary of UDBC is by far less than that on a vertical pile with the same diameter. Moreover, the ice force frequencies on upright and inve-rted cones are obtained under the field condition of the platform JZ20-2, respectively. The results show that the alterna-tion of the ice force on UDBC can hardly induce resonance of platform JZ20-2. 相似文献
98.
1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。 相似文献
99.
一、引 言 由于地形性激发的中尺度重力波的中断,在加拿大气候中心大气环流谱模式中有时候采用了波的曳力效应的参数化。这一参数化的细节以及它在大气环流模式气候模拟中影响的讨论,可见Boer等人(1985)的工作。 最近,人们利用激发重力波次尺度地形的更好的描绘,来修改这一参数化方案。还有,使用Lindzen(1981)的波饱和概念来描述曳力效应。这一关于地形重力波曳力参数化的作法已在加拿大气候中心的大气环流谱模式中广泛试验,在加拿大气象中心的业务的谱数值预报模式中也正在普遍进行试验。本文的目的所在于概述在这些模式中正在普遍使用的波的曳力方案,并且证明这一方案在消除中期预报和大气环流模拟的系统性误差方面的用处。 相似文献
100.